Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973403708> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1973403708 endingPage "26" @default.
- W1973403708 startingPage "18" @default.
- W1973403708 abstract "A new method for 3H-labeling of native collagen and a specific microassay for collagenase activity are presented. Acid-soluble type I collagen derived from rat tail tendons was reacted with pyridoxal phosphate and then reduced with NaB3H4 to yield [3H]collagen with a specific activity of more than 10 μCi/mg. With respect to rate of hydrolysis, trypsin susceptibility, and gelling properties this collagen compares favorably with biosynthetically labeled preparations. It was shown that chemical labeling procedures such as this, or N-acetylation with acetic anhydride, do not adversely affect properties of collagen which are important for its use as substrate in specific assays. The microassay employs 50-μl [3H]collagen gels (1 mg/ml) dispensed in microtest plates. At 36°C this assay combines rapid rate of hydrolysis with low trypsin susceptibility. As little as 1 ng of clostridial collagenase activity can be measured reproducibly. The high specific activity of the [3H]collagen allowed us to explore microassay conditions employing minute quantities of substrate in solution. These studies indicated that native type I collagen whether labeled or not, is cleaved in the helical region by trypsin at subdenaturation temperatures. It was concluded that, in order to remain specific, collagenase assays with collagen in solution as with collagen in fibrils must be performed at 10–12°C below the denaturation temperature, i.e., at 35–37°C with collagen gels and 27–29°C with collagen in solution." @default.
- W1973403708 created "2016-06-24" @default.
- W1973403708 creator A5011022273 @default.
- W1973403708 creator A5087918975 @default.
- W1973403708 date "1981-07-01" @default.
- W1973403708 modified "2023-10-14" @default.
- W1973403708 title "A sensitive collagenase assay using [3H]collagen labeled by reaction with pyridoxal phosphate and [3H]borohydride" @default.
- W1973403708 cites W1483228873 @default.
- W1973403708 cites W1578729690 @default.
- W1973403708 cites W1581445717 @default.
- W1973403708 cites W1968741611 @default.
- W1973403708 cites W1991479088 @default.
- W1973403708 cites W2008054230 @default.
- W1973403708 cites W2020471728 @default.
- W1973403708 cites W2056174236 @default.
- W1973403708 cites W2067647381 @default.
- W1973403708 cites W2068767837 @default.
- W1973403708 cites W2072427904 @default.
- W1973403708 cites W2072545366 @default.
- W1973403708 cites W2119964134 @default.
- W1973403708 cites W2579546292 @default.
- W1973403708 doi "https://doi.org/10.1016/0003-2697(81)90515-7" @default.
- W1973403708 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6272601" @default.
- W1973403708 hasPublicationYear "1981" @default.
- W1973403708 type Work @default.
- W1973403708 sameAs 1973403708 @default.
- W1973403708 citedByCount "43" @default.
- W1973403708 countsByYear W19734037082012 @default.
- W1973403708 crossrefType "journal-article" @default.
- W1973403708 hasAuthorship W1973403708A5011022273 @default.
- W1973403708 hasAuthorship W1973403708A5087918975 @default.
- W1973403708 hasConcept C134018914 @default.
- W1973403708 hasConcept C13965031 @default.
- W1973403708 hasConcept C161790260 @default.
- W1973403708 hasConcept C181199279 @default.
- W1973403708 hasConcept C185592680 @default.
- W1973403708 hasConcept C18903297 @default.
- W1973403708 hasConcept C197957613 @default.
- W1973403708 hasConcept C2776834122 @default.
- W1973403708 hasConcept C2776923230 @default.
- W1973403708 hasConcept C2777289219 @default.
- W1973403708 hasConcept C2778394429 @default.
- W1973403708 hasConcept C2778931688 @default.
- W1973403708 hasConcept C2779851118 @default.
- W1973403708 hasConcept C2779854550 @default.
- W1973403708 hasConcept C2780340462 @default.
- W1973403708 hasConcept C2780644872 @default.
- W1973403708 hasConcept C2780859938 @default.
- W1973403708 hasConcept C43617362 @default.
- W1973403708 hasConcept C55493867 @default.
- W1973403708 hasConcept C86803240 @default.
- W1973403708 hasConcept C94412978 @default.
- W1973403708 hasConceptScore W1973403708C134018914 @default.
- W1973403708 hasConceptScore W1973403708C13965031 @default.
- W1973403708 hasConceptScore W1973403708C161790260 @default.
- W1973403708 hasConceptScore W1973403708C181199279 @default.
- W1973403708 hasConceptScore W1973403708C185592680 @default.
- W1973403708 hasConceptScore W1973403708C18903297 @default.
- W1973403708 hasConceptScore W1973403708C197957613 @default.
- W1973403708 hasConceptScore W1973403708C2776834122 @default.
- W1973403708 hasConceptScore W1973403708C2776923230 @default.
- W1973403708 hasConceptScore W1973403708C2777289219 @default.
- W1973403708 hasConceptScore W1973403708C2778394429 @default.
- W1973403708 hasConceptScore W1973403708C2778931688 @default.
- W1973403708 hasConceptScore W1973403708C2779851118 @default.
- W1973403708 hasConceptScore W1973403708C2779854550 @default.
- W1973403708 hasConceptScore W1973403708C2780340462 @default.
- W1973403708 hasConceptScore W1973403708C2780644872 @default.
- W1973403708 hasConceptScore W1973403708C2780859938 @default.
- W1973403708 hasConceptScore W1973403708C43617362 @default.
- W1973403708 hasConceptScore W1973403708C55493867 @default.
- W1973403708 hasConceptScore W1973403708C86803240 @default.
- W1973403708 hasConceptScore W1973403708C94412978 @default.
- W1973403708 hasIssue "1" @default.
- W1973403708 hasLocation W19734037081 @default.
- W1973403708 hasLocation W19734037082 @default.
- W1973403708 hasOpenAccess W1973403708 @default.
- W1973403708 hasPrimaryLocation W19734037081 @default.
- W1973403708 hasRelatedWork W157820598 @default.
- W1973403708 hasRelatedWork W1966567387 @default.
- W1973403708 hasRelatedWork W1973403708 @default.
- W1973403708 hasRelatedWork W1995493762 @default.
- W1973403708 hasRelatedWork W2002403511 @default.
- W1973403708 hasRelatedWork W2040321109 @default.
- W1973403708 hasRelatedWork W2091472909 @default.
- W1973403708 hasRelatedWork W2122058980 @default.
- W1973403708 hasRelatedWork W2811032335 @default.
- W1973403708 hasRelatedWork W3151743302 @default.
- W1973403708 hasVolume "115" @default.
- W1973403708 isParatext "false" @default.
- W1973403708 isRetracted "false" @default.
- W1973403708 magId "1973403708" @default.
- W1973403708 workType "article" @default.