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- W1973408030 abstract "Photolithography was used to pattern hydrophobic design on common quantitative filter paper, and then the paper-based micro-zone plates were successfully fabricated. In order to improve its protein-adsorbing capacity for further signal magnification, modified silicon dioxide (SiO2) micro-beads were deposited onto the surface of paper. Indirect-ELISA for goat anti-rabbit IgG was realized on the 36-zone plates. Taking enzyme-linked goat anti-rabbit IgG as an example, loading SiO2 beads could remarkably increase the quantity of the adsorbed protein by 20%–700%. When this method was used for goat anti-rabbit IgG detection, signals were magnified by 20%–150%, and the linear range of detection was from 3 × 10−12 mmol to 3 × 10−8 mmol per zone. In this manner, bio-reagent consumption was reduced to 3–5 μL per zone, test time dropped to 25 min per plate and the cost of fabrication was reduced to $1.1 per plate. This work suggests a new way for development of new diagnosis devices." @default.
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- W1973408030 date "2013-01-01" @default.
- W1973408030 modified "2023-09-23" @default.
- W1973408030 title "Study on Enzyme Linked Immunosorbent Assay Using Paper-based Micro-zone Plates" @default.
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- W1973408030 doi "https://doi.org/10.1016/s1872-2040(13)60620-9" @default.
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