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- W1973414324 abstract "The mitosomes of Giardia intestinalis are thought to be mitochondria highly-reduced in response to the oxygen-poor niche. We performed a quantitative proteomic assessment of Giardia mitosomes to increase understanding of the function and evolutionary origin of these enigmatic organelles. Mitosome-enriched fractions were obtained from cell homogenate using Optiprep gradient centrifugation. To distinguish mitosomal proteins from contamination, we used a quantitative shot-gun strategy based on isobaric tagging of peptides with iTRAQ and tandem mass spectrometry. Altogether, 638 proteins were identified in mitosome-enriched fractions. Of these, 139 proteins had iTRAQ ratio similar to that of the six known mitosomal markers. Proteins were selected for expression in Giardia to verify their cellular localizations and the mitosomal localization of 20 proteins was confirmed. These proteins include nine components of the FeS cluster assembly machinery, a novel diflavo-protein with NADPH reductase activity, a novel VAMP-associated protein, and a key component of the outer membrane protein translocase. None of the novel mitosomal proteins was predicted by previous genome analyses. The small proteome of the Giardia mitosome reflects the reduction in mitochondrial metabolism, which is limited to the FeS cluster assembly pathway, and a simplicity in the protein import pathway required for organelle biogenesis." @default.
- W1973414324 created "2016-06-24" @default.
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- W1973414324 date "2011-02-24" @default.
- W1973414324 modified "2023-10-16" @default.
- W1973414324 title "The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis" @default.
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- W1973414324 doi "https://doi.org/10.1371/journal.pone.0017285" @default.
- W1973414324 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3044749" @default.
- W1973414324 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21390322" @default.
- W1973414324 hasPublicationYear "2011" @default.
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