Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973460794> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1973460794 abstract "The role of the aminophospholipid, phosphatidylethanolamine (PE), has been well established to act as a non-protein molecular chaperone in the folding and assembly of polytopic membrane proteins. However, such studies with soluble proteins have not been done so far and in particular with the heme proteins. We have used the heme enzyme, horseradish peroxidase (HRP), as the model heme protein and studied the effect of different phospholipids on its refolding from denatured state. Dimyristoylphosphatidylethanolamine (DMPE), a bilayer-forming PE, was able to increase the reactivation yield of denatured HRP upon 30min refolding at 25 degrees C. However, dioleoylphosphatidylethanolamine (DOPE), containing one double bond in the fatty acid chains, which does not favour bilayer organization, did not support proper refolding. The phospholipids with N-methylated head groups, phosphatidylcholines, e.g., DMPC and DOPC showed differential effects when DMPC remained mostly non-supportive while DOPC on the contrary led to inhibition of the refolding of the denatured heme enzyme. Fluorescence spectroscopic studies also indicated changes in the microenvironments of the heme moiety and the single tryptophan residue of HRP in presence of the aminophospholipid." @default.
- W1973460794 created "2016-06-24" @default.
- W1973460794 creator A5068924926 @default.
- W1973460794 creator A5072894280 @default.
- W1973460794 creator A5091522212 @default.
- W1973460794 date "2003-02-01" @default.
- W1973460794 modified "2023-09-27" @default.
- W1973460794 title "Phospholipid assisted folding of a denatured heme protein: effect of phosphatidylethanolamine" @default.
- W1973460794 cites W1546416744 @default.
- W1973460794 cites W1582395028 @default.
- W1973460794 cites W1601666612 @default.
- W1973460794 cites W1965918112 @default.
- W1973460794 cites W1967185063 @default.
- W1973460794 cites W1985260080 @default.
- W1973460794 cites W1995739053 @default.
- W1973460794 cites W2013130041 @default.
- W1973460794 cites W2022849714 @default.
- W1973460794 cites W2024581308 @default.
- W1973460794 cites W2029926648 @default.
- W1973460794 cites W2034653493 @default.
- W1973460794 cites W2035172570 @default.
- W1973460794 cites W2035247586 @default.
- W1973460794 cites W2053514414 @default.
- W1973460794 cites W2068050620 @default.
- W1973460794 cites W2072578069 @default.
- W1973460794 cites W2081675330 @default.
- W1973460794 cites W2082072901 @default.
- W1973460794 cites W2086382728 @default.
- W1973460794 cites W2112374350 @default.
- W1973460794 cites W2113301046 @default.
- W1973460794 cites W2165898505 @default.
- W1973460794 cites W2253374107 @default.
- W1973460794 cites W268238884 @default.
- W1973460794 doi "https://doi.org/10.1016/s0006-291x(03)00066-4" @default.
- W1973460794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12589809" @default.
- W1973460794 hasPublicationYear "2003" @default.
- W1973460794 type Work @default.
- W1973460794 sameAs 1973460794 @default.
- W1973460794 citedByCount "9" @default.
- W1973460794 countsByYear W19734607942012 @default.
- W1973460794 countsByYear W19734607942016 @default.
- W1973460794 countsByYear W19734607942020 @default.
- W1973460794 crossrefType "journal-article" @default.
- W1973460794 hasAuthorship W1973460794A5068924926 @default.
- W1973460794 hasAuthorship W1973460794A5072894280 @default.
- W1973460794 hasAuthorship W1973460794A5091522212 @default.
- W1973460794 hasConcept C12554922 @default.
- W1973460794 hasConcept C181199279 @default.
- W1973460794 hasConcept C185592680 @default.
- W1973460794 hasConcept C192157962 @default.
- W1973460794 hasConcept C204328495 @default.
- W1973460794 hasConcept C2776217839 @default.
- W1973460794 hasConcept C2776330855 @default.
- W1973460794 hasConcept C2776568683 @default.
- W1973460794 hasConcept C2777676563 @default.
- W1973460794 hasConcept C2778918659 @default.
- W1973460794 hasConcept C2781170229 @default.
- W1973460794 hasConcept C39944091 @default.
- W1973460794 hasConcept C41625074 @default.
- W1973460794 hasConcept C55493867 @default.
- W1973460794 hasConcept C71240020 @default.
- W1973460794 hasConcept C86803240 @default.
- W1973460794 hasConceptScore W1973460794C12554922 @default.
- W1973460794 hasConceptScore W1973460794C181199279 @default.
- W1973460794 hasConceptScore W1973460794C185592680 @default.
- W1973460794 hasConceptScore W1973460794C192157962 @default.
- W1973460794 hasConceptScore W1973460794C204328495 @default.
- W1973460794 hasConceptScore W1973460794C2776217839 @default.
- W1973460794 hasConceptScore W1973460794C2776330855 @default.
- W1973460794 hasConceptScore W1973460794C2776568683 @default.
- W1973460794 hasConceptScore W1973460794C2777676563 @default.
- W1973460794 hasConceptScore W1973460794C2778918659 @default.
- W1973460794 hasConceptScore W1973460794C2781170229 @default.
- W1973460794 hasConceptScore W1973460794C39944091 @default.
- W1973460794 hasConceptScore W1973460794C41625074 @default.
- W1973460794 hasConceptScore W1973460794C55493867 @default.
- W1973460794 hasConceptScore W1973460794C71240020 @default.
- W1973460794 hasConceptScore W1973460794C86803240 @default.
- W1973460794 hasLocation W19734607941 @default.
- W1973460794 hasLocation W19734607942 @default.
- W1973460794 hasOpenAccess W1973460794 @default.
- W1973460794 hasPrimaryLocation W19734607941 @default.
- W1973460794 hasRelatedWork W1972451253 @default.
- W1973460794 hasRelatedWork W2004685675 @default.
- W1973460794 hasRelatedWork W2011584036 @default.
- W1973460794 hasRelatedWork W2055770621 @default.
- W1973460794 hasRelatedWork W2069021409 @default.
- W1973460794 hasRelatedWork W2107837795 @default.
- W1973460794 hasRelatedWork W2115355207 @default.
- W1973460794 hasRelatedWork W2161068666 @default.
- W1973460794 hasRelatedWork W2538099234 @default.
- W1973460794 hasRelatedWork W3209321176 @default.
- W1973460794 isParatext "false" @default.
- W1973460794 isRetracted "false" @default.
- W1973460794 magId "1973460794" @default.
- W1973460794 workType "article" @default.