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• W1973462692 abstract "Aim To investigate the cytotoxicity of BioAggregate and iRoot BP Plus root canal sealer (iRoot BP Plus) to human dental pulp cells (hDPCs) and their effect on proliferation and mineralization of hDPCs and to compare their performance with that of mineral trioxide aggregate (MTA). Methodology Human dental pulp cells were seeded onto the prepared BioAggregate, iRoot BP Plus and MTA, respectively. Cell proliferation was assessed by CCK-8 cell proliferation kit on days 1, 3, 5 and 7. ALP activity was measured to evaluate the cell differentiation potential on days 1, 3, 5 and 7. The expression of odontoblastic differentiation-related genes (dentine phosphoprotein, dentine matrix protein-1 and osteocalcin) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Statistical tests used were one-way anova and post hoc Tukey's test. Results The proliferation of hDPCs in the MTA group was suppressed throughout the culture period, whereas the BioAggregate group and the iRoot BP Plus group first significantly increased cell numbers on day 1 (P < 0.01) and then decreased on day 3 to day 7. ALP activity was enhanced in all the three groups from day 3, whilst iRoot BP Plus showed significantly higher ALP activity than MTA (P < 0.01). qRT-PCR indicated that both BioAggregate and iRoot BP Plus groups were associated with a higher upregulation of mineralization and odontoblastic differentiation-associated gene expressions as compared to MTA group (P < 0.05). Conclusions BioAggregate and iRoot BP Plus were nontoxic and able to induce mineralization and odontoblastic differentiation-associated gene expression in hDPCs." @default.
• W1973462692 created "2016-06-24" @default.
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• W1973462692 date "2013-03-11" @default.
• W1973462692 modified "2023-10-12" @default.
• W1973462692 title "BioAggregate and iRoot BP Plus optimize the proliferation and mineralization ability of human dental pulp cells" @default.
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• W1973462692 doi "https://doi.org/10.1111/iej.12082" @default.
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