Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973488340> ?p ?o ?g. }
- W1973488340 endingPage "1489" @default.
- W1973488340 startingPage "1477" @default.
- W1973488340 abstract "ABSTRACT Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [QS]). The basic design of these Escherichia coli -based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ.1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ.1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum-quenching (QQ) activities using the established reporter systems AI1-QQ.1 and AI2-QQ.1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates ( Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus ). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes." @default.
- W1973488340 created "2016-06-24" @default.
- W1973488340 creator A5055322220 @default.
- W1973488340 creator A5086005113 @default.
- W1973488340 creator A5088021377 @default.
- W1973488340 date "2015-02-15" @default.
- W1973488340 modified "2023-10-12" @default.
- W1973488340 title "Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing" @default.
- W1973488340 cites W1242779636 @default.
- W1973488340 cites W1499349857 @default.
- W1973488340 cites W1511335270 @default.
- W1973488340 cites W1518158556 @default.
- W1973488340 cites W1703657909 @default.
- W1973488340 cites W1763857691 @default.
- W1973488340 cites W1826058912 @default.
- W1973488340 cites W1831103454 @default.
- W1973488340 cites W1837250543 @default.
- W1973488340 cites W1851892282 @default.
- W1973488340 cites W1861122134 @default.
- W1973488340 cites W1959548112 @default.
- W1973488340 cites W1967478782 @default.
- W1973488340 cites W1968303386 @default.
- W1973488340 cites W1972038449 @default.
- W1973488340 cites W1975993749 @default.
- W1973488340 cites W1983030906 @default.
- W1973488340 cites W1984247855 @default.
- W1973488340 cites W1985122242 @default.
- W1973488340 cites W1987535485 @default.
- W1973488340 cites W1991125394 @default.
- W1973488340 cites W1994402597 @default.
- W1973488340 cites W1996645913 @default.
- W1973488340 cites W1997118588 @default.
- W1973488340 cites W1999851893 @default.
- W1973488340 cites W2001760607 @default.
- W1973488340 cites W2004280279 @default.
- W1973488340 cites W2005255801 @default.
- W1973488340 cites W2008363547 @default.
- W1973488340 cites W2009438634 @default.
- W1973488340 cites W2009511198 @default.
- W1973488340 cites W2014004132 @default.
- W1973488340 cites W2016957700 @default.
- W1973488340 cites W2022358488 @default.
- W1973488340 cites W2022678082 @default.
- W1973488340 cites W2022959719 @default.
- W1973488340 cites W2026548787 @default.
- W1973488340 cites W2034719937 @default.
- W1973488340 cites W2035337053 @default.
- W1973488340 cites W2036543661 @default.
- W1973488340 cites W2039584791 @default.
- W1973488340 cites W2041854993 @default.
- W1973488340 cites W2043723029 @default.
- W1973488340 cites W2047850740 @default.
- W1973488340 cites W2052043623 @default.
- W1973488340 cites W2059979846 @default.
- W1973488340 cites W2064754363 @default.
- W1973488340 cites W2066784459 @default.
- W1973488340 cites W2071143880 @default.
- W1973488340 cites W2071144001 @default.
- W1973488340 cites W2072591481 @default.
- W1973488340 cites W2072936433 @default.
- W1973488340 cites W2075407586 @default.
- W1973488340 cites W2076356586 @default.
- W1973488340 cites W2077627110 @default.
- W1973488340 cites W2079698171 @default.
- W1973488340 cites W2091446851 @default.
- W1973488340 cites W2093247191 @default.
- W1973488340 cites W2098064749 @default.
- W1973488340 cites W2098894017 @default.
- W1973488340 cites W2100723520 @default.
- W1973488340 cites W2103167380 @default.
- W1973488340 cites W2104198286 @default.
- W1973488340 cites W2107804628 @default.
- W1973488340 cites W2108808146 @default.
- W1973488340 cites W2108808336 @default.
- W1973488340 cites W2111555302 @default.
- W1973488340 cites W2112001696 @default.
- W1973488340 cites W2113198717 @default.
- W1973488340 cites W2113490404 @default.
- W1973488340 cites W2114591302 @default.
- W1973488340 cites W2119410664 @default.
- W1973488340 cites W2119634475 @default.
- W1973488340 cites W2122608498 @default.
- W1973488340 cites W2124193678 @default.
- W1973488340 cites W2124517799 @default.
- W1973488340 cites W2125268958 @default.
- W1973488340 cites W2127347791 @default.
- W1973488340 cites W2127438500 @default.
- W1973488340 cites W2132417094 @default.
- W1973488340 cites W2134039538 @default.
- W1973488340 cites W2134153749 @default.
- W1973488340 cites W2135371345 @default.
- W1973488340 cites W2135876243 @default.
- W1973488340 cites W2136035133 @default.
- W1973488340 cites W2136294927 @default.
- W1973488340 cites W2137659876 @default.
- W1973488340 cites W2141368045 @default.
- W1973488340 cites W2141917119 @default.
- W1973488340 cites W2142963330 @default.