FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973508997> ?p ?o ?g. }

• W1973508997 endingPage "535" @default.
• W1973508997 startingPage "524" @default.
• W1973508997 abstract "The recovery of the enzyme poly(ADP-ribose) polymerase (pADPRp) in the nuclease- and 1.6 M NaCl-resistant nuclear subfraction prepared from a number of different sources was assessed by Western blotting. When rat liver nuclei were treated with DNase I and RNase A followed by 1.6 M NaCl, approximately 10% of the nuclear pADPRp was recovered in the sedimentable fraction. The proportion of pADPRp recovered with the residual fraction decreased to less than 5% of the total nuclear polymerase when nuclei were prepared in the presence of the sulfhydryl blocking reagent iodoacetamide and increased to approximately 50% of the total nuclear pADPRp when nuclei were treated with the sulfhydryl cross-linking reagent sodium tetrathionate (NaTT) prior to fractionation. To determine whether this effect of disulfide bond formation was unique to rat liver nuclei, nuclear matrix/cytoskeleton structures were prepared in situ by sequentially treating monolayers of tissue culture cells with Nonidet-P40, DNase I and RNase A, and 1.6 M NaCl (S.H. Kaufmann and J.H. Shaper (1991) Exp. Cell Res. 192, 511-523). When nuclear monolayers were prepared from HTC rat hepatoma cells, CaLu-1 human lung carcinoma cells, and CHO hamster ovary cells in the absence of NaTT, pADPRp was undetectable in the nuclease- and 1.6 M NaCl-resistant fraction. In contrast, when nuclear monolayers were isolated in the presence of NaTT, from 5% (CaLu-1) to 26% (HTC cells) of the total nuclear pADPRp was recovered with the nuclease- and salt-resistant fraction. Examination of these residual structures by SDS-polyacrylamide gel electrophoresis under nonreducing conditions suggested that pADPRp was present as a component of disulfide cross-linked complexes. Further analysis by immunofluorescence revealed that the pADPRp was diffusely distributed throughout the CaLu-1 or CHO nuclear matrix. In addition, when matrices were prepared in the absence of RNase A, pADPRp was also observed in the residual nucleoli. These observations reveal that the recovery of pADPRp with a nuclease- and salt-resistant nuclear subfraction is dependent on the source of the nuclei and on the conditions used to fractionate those nuclei. In addition, these observations raise the possibility that there might be different functional classes of pADPRp molecules within the nucleus." @default.
• W1973508997 created "2016-06-24" @default.
• W1973508997 creator A5014174818 @default.
• W1973508997 creator A5019945861 @default.
• W1973508997 creator A5035695904 @default.
• W1973508997 creator A5054370710 @default.
• W1973508997 creator A5063766878 @default.
• W1973508997 creator A5065856436 @default.
• W1973508997 creator A5069507613 @default.
• W1973508997 date "1991-02-01" @default.
• W1973508997 modified "2023-10-18" @default.
• W1973508997 title "Association of poly(ADP-ribose) polymerase with the nuclear matrix: The role intermolecular disulfide bond formation, RNA retention, and cell type" @default.
• W1973508997 cites W1479854695 @default.
• W1973508997 cites W1496754597 @default.
• W1973508997 cites W1498308778 @default.
• W1973508997 cites W1510596204 @default.
• W1973508997 cites W1512197606 @default.
• W1973508997 cites W1514826684 @default.
• W1973508997 cites W1515462367 @default.
• W1973508997 cites W1528696948 @default.
• W1973508997 cites W1558998530 @default.
• W1973508997 cites W1587229711 @default.
• W1973508997 cites W1589365860 @default.
• W1973508997 cites W1593421490 @default.
• W1973508997 cites W1625856192 @default.
• W1973508997 cites W1839165938 @default.
• W1973508997 cites W1848424641 @default.
• W1973508997 cites W1849335414 @default.
• W1973508997 cites W1964634994 @default.
• W1973508997 cites W1968650322 @default.
• W1973508997 cites W1977276762 @default.
• W1973508997 cites W1979820735 @default.
• W1973508997 cites W1980362791 @default.
• W1973508997 cites W1992503056 @default.
• W1973508997 cites W1993341657 @default.
• W1973508997 cites W2003290749 @default.
• W1973508997 cites W2006942905 @default.
• W1973508997 cites W2019789451 @default.
• W1973508997 cites W2027637631 @default.
• W1973508997 cites W2030366638 @default.
• W1973508997 cites W2030905726 @default.
• W1973508997 cites W2031725750 @default.
• W1973508997 cites W2043734426 @default.
• W1973508997 cites W2055551468 @default.
• W1973508997 cites W2057940505 @default.
• W1973508997 cites W2071796546 @default.
• W1973508997 cites W2076045005 @default.
• W1973508997 cites W2089897298 @default.
• W1973508997 cites W2091506114 @default.
• W1973508997 cites W2092837591 @default.
• W1973508997 cites W2094424604 @default.
• W1973508997 cites W2114427556 @default.
• W1973508997 cites W2123883912 @default.
• W1973508997 cites W2129059884 @default.
• W1973508997 cites W2144573522 @default.
• W1973508997 cites W3180905028 @default.
• W1973508997 cites W943460054 @default.
• W1973508997 doi "https://doi.org/10.1016/0014-4827(91)90072-3" @default.
• W1973508997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1703086" @default.
• W1973508997 hasPublicationYear "1991" @default.
• W1973508997 type Work @default.
• W1973508997 sameAs 1973508997 @default.
• W1973508997 citedByCount "54" @default.
• W1973508997 countsByYear W19735089972012 @default.
• W1973508997 countsByYear W19735089972013 @default.
• W1973508997 countsByYear W19735089972014 @default.
• W1973508997 countsByYear W19735089972016 @default.
• W1973508997 countsByYear W19735089972020 @default.
• W1973508997 countsByYear W19735089972022 @default.
• W1973508997 crossrefType "journal-article" @default.
• W1973508997 hasAuthorship W1973508997A5014174818 @default.
• W1973508997 hasAuthorship W1973508997A5019945861 @default.
• W1973508997 hasAuthorship W1973508997A5035695904 @default.
• W1973508997 hasAuthorship W1973508997A5054370710 @default.
• W1973508997 hasAuthorship W1973508997A5063766878 @default.
• W1973508997 hasAuthorship W1973508997A5065856436 @default.
• W1973508997 hasAuthorship W1973508997A5069507613 @default.
• W1973508997 hasConcept C104317684 @default.
• W1973508997 hasConcept C105686024 @default.
• W1973508997 hasConcept C10879258 @default.
• W1973508997 hasConcept C153911025 @default.
• W1973508997 hasConcept C181199279 @default.
• W1973508997 hasConcept C182979987 @default.
• W1973508997 hasConcept C2776515853 @default.
• W1973508997 hasConcept C2777271071 @default.
• W1973508997 hasConcept C2779201268 @default.
• W1973508997 hasConcept C2780114586 @default.
• W1973508997 hasConcept C552990157 @default.
• W1973508997 hasConcept C55493867 @default.
• W1973508997 hasConcept C67705224 @default.
• W1973508997 hasConcept C82381507 @default.
• W1973508997 hasConcept C83640560 @default.
• W1973508997 hasConcept C86803240 @default.
• W1973508997 hasConceptScore W1973508997C104317684 @default.
• W1973508997 hasConceptScore W1973508997C105686024 @default.
• W1973508997 hasConceptScore W1973508997C10879258 @default.
• W1973508997 hasConceptScore W1973508997C153911025 @default.
• W1973508997 hasConceptScore W1973508997C181199279 @default.

• next