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- W1973577462 abstract "A simple and sensitive fluorometric high-performance liquid chromatographic method was developed for the determination of midodrine in human plasma. After liquid-liquid extraction from plasma, the drug and 2-phenylglycinol (internal standard) were convened into the corresponding fluorescent derivatives by reaction with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives were separated within 30 min on a reversed-phase column using isocratic elution with acetonitrile-methanol-water (10:30:60, v/v) and were detected spectrofluorometrically at 485 nm with excitation at 400 nm. The detection limit for midodrine was 0.3 pmol (76 pg) per mL plasma at a signal-to-noise ratio of 3." @default.
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- W1973577462 date "2003-02-01" @default.
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- W1973577462 title "Determination of Midodrine in Human Plasma by High-Performance Liquid Chromatography with Fluorescence Detection" @default.
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- W1973577462 doi "https://doi.org/10.2116/analsci.19.317" @default.
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