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- W1973600151 abstract "A densitometric method that permits the determination of relative amounts and synthesis rates of proteins separated by two-dimensional gel electrophoresis is presented. The method is applicable to proteins that have been stained by Coomassie blue or proteins that have been radiolabeled. The analysis is achieved by scanning selected spots with an ordinary densitometer in the horizontal and vertical direction and relating the response to the volume of an ellipsoid. A linear dependence is observed between the amount of protein or incorporated radioactivity and the measured optical density. The advantage of this method is that specialized scanning instruments and computer analysis are not required. The method is most useful for the analysis of a few specific proteins which change in their relative amount or specific activity due to experimental manipulations. Difficulties in the analysis of protein spots derived from the twodimensional gel electrophoresis technique are discussed and compared to an analysis of bands from the one-dimensional electrophoresis technique." @default.
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- W1973600151 date "1982-08-01" @default.
- W1973600151 modified "2023-09-27" @default.
- W1973600151 title "A noncomputerized scanning method for determining relative protein quantities and synthesis rates on two-dimensional electrophoretic gels" @default.
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- W1973600151 doi "https://doi.org/10.1016/0003-2697(82)90033-1" @default.
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