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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973604889> ?p ?o ?g. }

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• W1973604889 abstract "The cloning in Escherichia coli of the 3′ moieties of the cpl-1 gnes is described, for coding for the C-terminal regions of the lytic amidase of Streptococcus pneumoniae and the phage Cp-1 lysozyme, respectively. The truncated genes were overexpressed in E. coli and the purified polypeptides showed a great affinity for choline, although they were devoid of cell wall-degrading activity. Biochemical and circular dichroism analyses indicated that these are the domains responsible for the specific recognition of the choline-containing pneumococcal cell walls by the lytic enzymes. The data presented here suggested that these choline-binding domains can function independently of their catalytic domains." @default.
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• W1973604889 date "1990-04-01" @default.
• W1973604889 modified "2023-09-27" @default.
• W1973604889 title "Cloning and expression of gene fragments encoding the choline-binding domain of pneumococcal murein hydrolases" @default.
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• W1973604889 doi "https://doi.org/10.1016/0378-1119(90)90207-8" @default.
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