FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973605000> ?p ?o ?g. }

• W1973605000 endingPage "166" @default.
• W1973605000 startingPage "160" @default.
• W1973605000 abstract "Summary ADAMTS-13 cleaves large and ultra-large vonWillebrand factor multimers normally secreted by endothelial cells. Severe deficiency of this enzyme leads to thrombotic thrombocytopenic purpura (TTP). We applied the Impact-R system [Cone and plate(let) Analyzer, CPA] to determine optimal conditions for ADAMTS-13 function, to assess it’s activity in TTP patients and to distinguish inheritedTTP (inTTP) from acquiredTTP (acTTP). The role of ADAMTS-13 in platelet adhesion under different conditions was investigated applying recombinant forms of VWF and ADAMTS-13. rVWF was first treated by rADAMTS-13 either in solution or when immobilized on the surface of the well, under static or flow conditions, in the presence or absence of BaCl2. The resulting cleavedVWF fragments were then immobilized and served to assess type 3 von Willebrand disease whole blood platelet adhesion under flow. Maximal effect of the rADAMTS-13 (decrease of platelet adhesion in the absence compared to the presence of BaCl2), was observed when the rVWF was pre-immobilized and the cleavage step took place under flow (85%). Mixing plasma ofTTP patients with normal blood (1:3) yielded a 1.6 to 2-fold increase of platelet adhesion under flow compared to mixing normal plasma with normal blood, at shear rate range of 1,800–2,500s−1. Maximal increase of platelet adhesion was observed under 2,050s−1. Under these conditions, the extent of adhesion was similarly higher in patients with inTTP and acTTP versus control [surface coverage (SC) 14.5 ± 2.8% and 14.6 ± 2.5% vs. 7.4 ± 1.7%, respectively]. ADAMTS-13 activity measured by collagen-binding test was similarly low (4.2 ± 3.8% and 3.5 ± 2.4% vs. 72.2 ± 8.0%, respectively). An inverse correlation between SC and ADAMTS-13 activity was observed in a patient with inTTP assayed eight times during plasma infusion treatment. Addition of BaCl2 to the mixture of TTP plasma and normal whole blood yieldeda decrease in platelet adhesion in inTTP (by 51%) but not in acTTP. The lack of reduction of platelet adhesion in acTTP could presumably be due to the presence of ADAMTS-13 inhibitor in these patients. These results suggest that VWF immobilization and high shear flow yielded optimal conditions for ADAMTS-13 activity, and that introduction of BaCl2 in the Impact-R (CPA) test may be useful for differentiation between inherited and acquired TTP." @default.
• W1973605000 created "2016-06-24" @default.
• W1973605000 creator A5011208686 @default.
• W1973605000 creator A5021847528 @default.
• W1973605000 creator A5025226122 @default.
• W1973605000 creator A5026803588 @default.
• W1973605000 creator A5071353833 @default.
• W1973605000 creator A5077139130 @default.
• W1973605000 creator A5083103240 @default.
• W1973605000 date "2006-01-01" @default.
• W1973605000 modified "2023-09-26" @default.
• W1973605000 title "ADAMTS-13 regulates platelet adhesion under flow" @default.
• W1973605000 cites W122479260 @default.
• W1973605000 cites W164651796 @default.
• W1973605000 cites W1831354387 @default.
• W1973605000 cites W1964151860 @default.
• W1973605000 cites W1970454636 @default.
• W1973605000 cites W1991523524 @default.
• W1973605000 cites W2020077683 @default.
• W1973605000 cites W2030923262 @default.
• W1973605000 cites W2034225516 @default.
• W1973605000 cites W2038881117 @default.
• W1973605000 cites W2041065426 @default.
• W1973605000 cites W2042649971 @default.
• W1973605000 cites W2044505929 @default.
• W1973605000 cites W2056238936 @default.
• W1973605000 cites W2068421824 @default.
• W1973605000 cites W2076190124 @default.
• W1973605000 cites W2077403724 @default.
• W1973605000 cites W2078406257 @default.
• W1973605000 cites W2095482915 @default.
• W1973605000 cites W2099584367 @default.
• W1973605000 cites W2114604545 @default.
• W1973605000 cites W2118277673 @default.
• W1973605000 cites W2129289095 @default.
• W1973605000 cites W2151503986 @default.
• W1973605000 cites W2186521452 @default.
• W1973605000 cites W2312673225 @default.
• W1973605000 cites W2315229770 @default.
• W1973605000 cites W2408474403 @default.
• W1973605000 cites W2410395775 @default.
• W1973605000 cites W2462736682 @default.
• W1973605000 cites W2052389108 @default.
• W1973605000 doi "https://doi.org/10.1160/th06-01-0019" @default.
• W1973605000 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16894459" @default.
• W1973605000 hasPublicationYear "2006" @default.
• W1973605000 type Work @default.
• W1973605000 sameAs 1973605000 @default.
• W1973605000 citedByCount "10" @default.
• W1973605000 countsByYear W19736050002012 @default.
• W1973605000 countsByYear W19736050002013 @default.
• W1973605000 countsByYear W19736050002021 @default.
• W1973605000 crossrefType "journal-article" @default.
• W1973605000 hasAuthorship W1973605000A5011208686 @default.
• W1973605000 hasAuthorship W1973605000A5021847528 @default.
• W1973605000 hasAuthorship W1973605000A5025226122 @default.
• W1973605000 hasAuthorship W1973605000A5026803588 @default.
• W1973605000 hasAuthorship W1973605000A5071353833 @default.
• W1973605000 hasAuthorship W1973605000A5077139130 @default.
• W1973605000 hasAuthorship W1973605000A5083103240 @default.
• W1973605000 hasConcept C109523444 @default.
• W1973605000 hasConcept C126322002 @default.
• W1973605000 hasConcept C134018914 @default.
• W1973605000 hasConcept C1621761 @default.
• W1973605000 hasConcept C167814343 @default.
• W1973605000 hasConcept C178790620 @default.
• W1973605000 hasConcept C185592680 @default.
• W1973605000 hasConcept C203014093 @default.
• W1973605000 hasConcept C2776224515 @default.
• W1973605000 hasConcept C2778585876 @default.
• W1973605000 hasConcept C2778780528 @default.
• W1973605000 hasConcept C2779394231 @default.
• W1973605000 hasConcept C2780009322 @default.
• W1973605000 hasConcept C2993272671 @default.
• W1973605000 hasConcept C2993802102 @default.
• W1973605000 hasConcept C55728118 @default.
• W1973605000 hasConcept C71924100 @default.
• W1973605000 hasConcept C84416704 @default.
• W1973605000 hasConcept C89560881 @default.
• W1973605000 hasConceptScore W1973605000C109523444 @default.
• W1973605000 hasConceptScore W1973605000C126322002 @default.
• W1973605000 hasConceptScore W1973605000C134018914 @default.
• W1973605000 hasConceptScore W1973605000C1621761 @default.
• W1973605000 hasConceptScore W1973605000C167814343 @default.
• W1973605000 hasConceptScore W1973605000C178790620 @default.
• W1973605000 hasConceptScore W1973605000C185592680 @default.
• W1973605000 hasConceptScore W1973605000C203014093 @default.
• W1973605000 hasConceptScore W1973605000C2776224515 @default.
• W1973605000 hasConceptScore W1973605000C2778585876 @default.
• W1973605000 hasConceptScore W1973605000C2778780528 @default.
• W1973605000 hasConceptScore W1973605000C2779394231 @default.
• W1973605000 hasConceptScore W1973605000C2780009322 @default.
• W1973605000 hasConceptScore W1973605000C2993272671 @default.
• W1973605000 hasConceptScore W1973605000C2993802102 @default.
• W1973605000 hasConceptScore W1973605000C55728118 @default.
• W1973605000 hasConceptScore W1973605000C71924100 @default.
• W1973605000 hasConceptScore W1973605000C84416704 @default.
• W1973605000 hasConceptScore W1973605000C89560881 @default.

• next