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• W1973631331 abstract "In this study, we examined the impact of various environmental conditions on the expression of resistance–nodulation–division (RND) efflux pumps and outer membrane (OM) porins, two key determinants of Acinetobacter baumannii's intrinsic resistance, an organism known to cause various multidrug resistant infections in immunocompromised individuals. Quantitative RT-PCR was used to analyze the expression of adeB, adeG, and adeJ (genes encoding RND pumps) and 33 kDa, carO, and oprD (genes encoding OM porins) of A. baumannii ATCC19606T under different incubation temperatures (30, 37, and 42 °C) and in the presence of high osmolarity and salicylate. Downregulation of all three RND pumps was observed at 30 °C, while downregulation of all three porins tested was observed at increased osmolarity. Downregulation of RND efflux pumps, particularly AdeABC, was consistent with increased susceptibility to antibiotics that are substrates of this pump. Expression of the adeR response regulator gene of the AdeRS system, the activator of the AdeABC pump, was also analyzed. Our work shows that various environmental stress conditions can influence the expression of RND pumps and porins in A. baumannii ATCC19606T and thus may play a role in the modulation of its antibiotic resistance. Environmental stress and antibiotic resistance in Acinetobacter baumannii." @default.
• W1973631331 created "2016-06-24" @default.
• W1973631331 creator A5028360618 @default.
• W1973631331 creator A5085855518 @default.
• W1973631331 date "2014-07-01" @default.
• W1973631331 modified "2023-10-16" @default.
• W1973631331 title "Incubation temperature, osmolarity, and salicylate affect the expression of resistance-nodulation-division efflux pumps and outer membrane porins in<i>Acinetobacter baumannii</i>ATCC19606^T" @default.
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• W1973631331 doi "https://doi.org/10.1111/1574-6968.12530" @default.
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• W1973631331 hasPublicationYear "2014" @default.
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