Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973656856> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1973656856 endingPage "3034" @default.
- W1973656856 startingPage "3028" @default.
- W1973656856 abstract "The fate of the thiol proton coming from the ionization of the sulfhydryl group of GSH in the active site of glutathione transferase P1-1 has been studied. pH changes caused by the binding of GSH to the enzyme in the absence of any inorganic buffer indicate that the thiol proton leaves the active site when the binary complex is formed. The amount of protons released is stoichiometric to the amount of GSH thiolate formed in the G-site. The apparent pKa value for the bound GSH, calculated with this potentiometric approach, is 6.18 ± 0.09; very similar values are found by spectrophotometric (6.20 ± 0.12) and by kinetic (6.00 ± 0.08) experiments. Binding of S-hexylglutathione does not cause any proton release. Stopped-flow data obtained by means of an acid−base indicator show that the proton extrusion process (apparent t1/2 = 1.1 ± 0.1 ms at 15 °C) is not rate limiting in turnover (apparent t1/2 = 34 ± 4 ms at 15 °C). By comparing the kinetic behavior of three distinct events occurring during the binding of GSH to the enzyme, i.e., proton release, ionization of bound GSH and quenching of intrinsic fluorescence, it appears that the binding process follows a multistep mechanism possibly involving the conformational transition of a weak precomplex into the final Michaelis complex. This step is modulated by helix 2 motions and may be rate limiting at physiological GSH concentrations. These findings, coming from kinetic studies, are consistent with NMR data [Nicotra, M., Paci, M., Sette, M., Oakley, A. J., Parker, M. W., Lo Bello, M., Caccuri, A. M., Federici, G., and Ricci, G. (1998) Biochemistry 37, 3020−3027] and time-resolved fluorescence experiments [Stella, L., Caccuri, A. M., Rosato, N., Nicotra, M., Lo Bello, M., De Matteis, F., Mazzetti, A. P., Federici, G., and Ricci, G., manuscript in preparation]." @default.
- W1973656856 created "2016-06-24" @default.
- W1973656856 creator A5000877030 @default.
- W1973656856 creator A5009225717 @default.
- W1973656856 creator A5026410325 @default.
- W1973656856 creator A5037457228 @default.
- W1973656856 creator A5048917582 @default.
- W1973656856 creator A5049435911 @default.
- W1973656856 creator A5075406326 @default.
- W1973656856 creator A5087504067 @default.
- W1973656856 date "1998-02-12" @default.
- W1973656856 modified "2023-10-16" @default.
- W1973656856 title "Proton Release upon Glutathione Binding to Glutathione Transferase P1-1: Kinetic Analysis of a Multistep Glutathione Binding Process" @default.
- W1973656856 cites W1505146479 @default.
- W1973656856 cites W2010060878 @default.
- W1973656856 cites W2020947886 @default.
- W1973656856 cites W2107387967 @default.
- W1973656856 cites W2111860128 @default.
- W1973656856 cites W23248100 @default.
- W1973656856 doi "https://doi.org/10.1021/bi971903g" @default.
- W1973656856 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9485455" @default.
- W1973656856 hasPublicationYear "1998" @default.
- W1973656856 type Work @default.
- W1973656856 sameAs 1973656856 @default.
- W1973656856 citedByCount "48" @default.
- W1973656856 countsByYear W19736568562012 @default.
- W1973656856 countsByYear W19736568562013 @default.
- W1973656856 countsByYear W19736568562014 @default.
- W1973656856 countsByYear W19736568562015 @default.
- W1973656856 countsByYear W19736568562017 @default.
- W1973656856 countsByYear W19736568562019 @default.
- W1973656856 crossrefType "journal-article" @default.
- W1973656856 hasAuthorship W1973656856A5000877030 @default.
- W1973656856 hasAuthorship W1973656856A5009225717 @default.
- W1973656856 hasAuthorship W1973656856A5026410325 @default.
- W1973656856 hasAuthorship W1973656856A5037457228 @default.
- W1973656856 hasAuthorship W1973656856A5048917582 @default.
- W1973656856 hasAuthorship W1973656856A5049435911 @default.
- W1973656856 hasAuthorship W1973656856A5075406326 @default.
- W1973656856 hasAuthorship W1973656856A5087504067 @default.
- W1973656856 hasConcept C107824862 @default.
- W1973656856 hasConcept C121332964 @default.
- W1973656856 hasConcept C121745418 @default.
- W1973656856 hasConcept C181199279 @default.
- W1973656856 hasConcept C185592680 @default.
- W1973656856 hasConcept C2779179908 @default.
- W1973656856 hasConcept C538909803 @default.
- W1973656856 hasConcept C55493867 @default.
- W1973656856 hasConcept C62520636 @default.
- W1973656856 hasConcept C71240020 @default.
- W1973656856 hasConcept C91881484 @default.
- W1973656856 hasConceptScore W1973656856C107824862 @default.
- W1973656856 hasConceptScore W1973656856C121332964 @default.
- W1973656856 hasConceptScore W1973656856C121745418 @default.
- W1973656856 hasConceptScore W1973656856C181199279 @default.
- W1973656856 hasConceptScore W1973656856C185592680 @default.
- W1973656856 hasConceptScore W1973656856C2779179908 @default.
- W1973656856 hasConceptScore W1973656856C538909803 @default.
- W1973656856 hasConceptScore W1973656856C55493867 @default.
- W1973656856 hasConceptScore W1973656856C62520636 @default.
- W1973656856 hasConceptScore W1973656856C71240020 @default.
- W1973656856 hasConceptScore W1973656856C91881484 @default.
- W1973656856 hasIssue "9" @default.
- W1973656856 hasLocation W19736568561 @default.
- W1973656856 hasLocation W19736568562 @default.
- W1973656856 hasOpenAccess W1973656856 @default.
- W1973656856 hasPrimaryLocation W19736568561 @default.
- W1973656856 hasRelatedWork W1965319078 @default.
- W1973656856 hasRelatedWork W2007353191 @default.
- W1973656856 hasRelatedWork W2011051574 @default.
- W1973656856 hasRelatedWork W2044556412 @default.
- W1973656856 hasRelatedWork W2057521823 @default.
- W1973656856 hasRelatedWork W2096363308 @default.
- W1973656856 hasRelatedWork W2380161418 @default.
- W1973656856 hasRelatedWork W2994794337 @default.
- W1973656856 hasRelatedWork W7511043 @default.
- W1973656856 hasRelatedWork W2062015454 @default.
- W1973656856 hasVolume "37" @default.
- W1973656856 isParatext "false" @default.
- W1973656856 isRetracted "false" @default.
- W1973656856 magId "1973656856" @default.
- W1973656856 workType "article" @default.