Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973669576> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W1973669576 endingPage "388a" @default.
- W1973669576 startingPage "388a" @default.
- W1973669576 abstract "Metastable protein conformations play an important role in the folding process because such partially disordered states can be gates for the misfolding pathway, leading sometimes to pathological structures, like fibrous aggregates. High pressure is a very useful tool in the study of metastable states, since application of the pressure is fully reversible, contrary to chemical agents. FTIR spectroscopy allows us to follow simultaneously the secondary structure, the packing (tertiary structure) and the aggregation of the protein using the amide I, amide II and the 1616 cm-1 bands respectively. We performed a systematic study on the temperature-pressure phase diagram of lysozyme and found a two-step unfolding profile both in the pressure and temperature directions. Hydrogen/deuterium exchange results show evidence for the molten globule formation at 57°C @130 MPa and 580 MPa @30°C, which are considerable lower values than those of the complete unfolding (e.g. 75°C@130MPa).Refolding of the protein after pressure unfolding is a slow process, with a time constant in the range of hours. Partially refolded structures present in this time range have different aggregation propensity. The kinetics of the aggregation has a biexponential character with a time constants of 1060 and 8600 s at 40C ambient pressure. Analyzing the time dependence of the amide I band shape we found that the strengthening of the intermolecular hydrogen bond network was accompanied by decrease of the folded secondary structure content.Moderate pressure of 300 MPa was found to be able to dissociate the aggregates, while the secondary structure is not yet unfolded. This fact together with the small temperature effect on the aggregation kinetics suggests that the rate of the aggregation of the metastable conformations is determined by the high activation volume rather than the high activation energy." @default.
- W1973669576 created "2016-06-24" @default.
- W1973669576 creator A5048474775 @default.
- W1973669576 date "2009-02-01" @default.
- W1973669576 modified "2023-10-16" @default.
- W1973669576 title "Evidence For Metastable States Of Lysozyme Revealed By High Pressure FTIR Spectroscopy" @default.
- W1973669576 doi "https://doi.org/10.1016/j.bpj.2008.12.2900" @default.
- W1973669576 hasPublicationYear "2009" @default.
- W1973669576 type Work @default.
- W1973669576 sameAs 1973669576 @default.
- W1973669576 citedByCount "1" @default.
- W1973669576 countsByYear W19736695762015 @default.
- W1973669576 crossrefType "journal-article" @default.
- W1973669576 hasAuthorship W1973669576A5048474775 @default.
- W1973669576 hasBestOaLocation W19736695761 @default.
- W1973669576 hasConcept C113196181 @default.
- W1973669576 hasConcept C120665830 @default.
- W1973669576 hasConcept C121332964 @default.
- W1973669576 hasConcept C160892712 @default.
- W1973669576 hasConcept C178790620 @default.
- W1973669576 hasConcept C185592680 @default.
- W1973669576 hasConcept C192562407 @default.
- W1973669576 hasConcept C2776966407 @default.
- W1973669576 hasConcept C32891209 @default.
- W1973669576 hasConcept C43617362 @default.
- W1973669576 hasConcept C55493867 @default.
- W1973669576 hasConcept C62520636 @default.
- W1973669576 hasConcept C89464430 @default.
- W1973669576 hasConceptScore W1973669576C113196181 @default.
- W1973669576 hasConceptScore W1973669576C120665830 @default.
- W1973669576 hasConceptScore W1973669576C121332964 @default.
- W1973669576 hasConceptScore W1973669576C160892712 @default.
- W1973669576 hasConceptScore W1973669576C178790620 @default.
- W1973669576 hasConceptScore W1973669576C185592680 @default.
- W1973669576 hasConceptScore W1973669576C192562407 @default.
- W1973669576 hasConceptScore W1973669576C2776966407 @default.
- W1973669576 hasConceptScore W1973669576C32891209 @default.
- W1973669576 hasConceptScore W1973669576C43617362 @default.
- W1973669576 hasConceptScore W1973669576C55493867 @default.
- W1973669576 hasConceptScore W1973669576C62520636 @default.
- W1973669576 hasConceptScore W1973669576C89464430 @default.
- W1973669576 hasIssue "3" @default.
- W1973669576 hasLocation W19736695761 @default.
- W1973669576 hasOpenAccess W1973669576 @default.
- W1973669576 hasPrimaryLocation W19736695761 @default.
- W1973669576 hasRelatedWork W1504397873 @default.
- W1973669576 hasRelatedWork W1964303156 @default.
- W1973669576 hasRelatedWork W1973669576 @default.
- W1973669576 hasRelatedWork W2019588946 @default.
- W1973669576 hasRelatedWork W2069625612 @default.
- W1973669576 hasRelatedWork W2313998586 @default.
- W1973669576 hasRelatedWork W2624179846 @default.
- W1973669576 hasRelatedWork W2899084033 @default.
- W1973669576 hasRelatedWork W2974168386 @default.
- W1973669576 hasRelatedWork W3203645043 @default.
- W1973669576 hasVolume "96" @default.
- W1973669576 isParatext "false" @default.
- W1973669576 isRetracted "false" @default.
- W1973669576 magId "1973669576" @default.
- W1973669576 workType "article" @default.