FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973688819> ?p ?o ?g. }

• W1973688819 endingPage "113" @default.
• W1973688819 startingPage "107" @default.
• W1973688819 abstract "During its metabolism, vanadium is known to become associated with the iron storage protein, ferritin. To elucidate probable vanadium binding sites on the protein, VO2+ binding to mammalian ferritins was studied using site-directed mutagenesis and EPR spectroscopy. VO2+-apoferritin EPR spectra of human H-chain (100% H), L-chain (100% L), horse spleen (84% L, 16% H) and sheep spleen (45% L, 55% H) ferritins revealed the presence of alpha and beta VO2+ species in all the proteins, implying that the ligands for these species are conserved between the H- and L-chains. The alpha species is less stable than the beta species and decreases with increasing pH, demonstrating that the two species are not pH-related, a result contrary to earlier proposals. EPR spectra of site-directed HuHF variants of several residues conserved in H- and L-chain ferritins (Asp-131, Glu-134, His-118 and His-128) suggest that His-118 near the outer opening of the three-fold channel is probably a ligand for VO2+ and is responsible for the beta signals in the EPR spectrum. The data indicate that VO2+ does not bind to the Asp-131 and Glu-134 residues within the three-fold channels nor does it bind at the ferroxidase site residues Glu-62 or His-65 or at the putative nucleation site residues Glu-61,64,67. While the ferroxidase site is not a site for VO2+ binding, mutation of residues Glu-62 and His-65 of this site to Ala affects VO2+ binding at His-118, located some 17 A away. Thus, VO2+ spin probe studies provide a window on structural changes in ferritin not seen in most previous work and indicate that long-range effects caused by point mutations must be carefully considered when drawing conclusions from mutagenesis studies of the protein." @default.
• W1973688819 created "2016-06-24" @default.
• W1973688819 creator A5001560379 @default.
• W1973688819 creator A5006229927 @default.
• W1973688819 creator A5038410313 @default.
• W1973688819 creator A5056671277 @default.
• W1973688819 creator A5091821876 @default.
• W1973688819 date "2000-05-01" @default.
• W1973688819 modified "2023-10-16" @default.
• W1973688819 title "Vanadyl(IV) binding to mammalian ferritins. An EPR study aided by site-directed mutagenesis" @default.
• W1973688819 cites W1520572643 @default.
• W1973688819 cites W1545086647 @default.
• W1973688819 cites W1556929642 @default.
• W1973688819 cites W1580121000 @default.
• W1973688819 cites W1667065506 @default.
• W1973688819 cites W1966041739 @default.
• W1973688819 cites W1971084208 @default.
• W1973688819 cites W1980964234 @default.
• W1973688819 cites W1987119816 @default.
• W1973688819 cites W2002642272 @default.
• W1973688819 cites W2002843987 @default.
• W1973688819 cites W2012982874 @default.
• W1973688819 cites W2013382319 @default.
• W1973688819 cites W2025375159 @default.
• W1973688819 cites W2037279387 @default.
• W1973688819 cites W2040096402 @default.
• W1973688819 cites W2054071128 @default.
• W1973688819 cites W2059219903 @default.
• W1973688819 cites W2061653137 @default.
• W1973688819 cites W2062154198 @default.
• W1973688819 cites W2069044991 @default.
• W1973688819 cites W2073208856 @default.
• W1973688819 cites W2078503156 @default.
• W1973688819 cites W2083030007 @default.
• W1973688819 cites W2086567353 @default.
• W1973688819 cites W2092091200 @default.
• W1973688819 cites W2092818123 @default.
• W1973688819 cites W2093705050 @default.
• W1973688819 cites W2108816504 @default.
• W1973688819 cites W2109745203 @default.
• W1973688819 cites W2156605690 @default.
• W1973688819 cites W2203571191 @default.
• W1973688819 cites W2247704355 @default.
• W1973688819 cites W2248193020 @default.
• W1973688819 cites W23835451 @default.
• W1973688819 cites W4293247451 @default.
• W1973688819 cites W976052722 @default.
• W1973688819 doi "https://doi.org/10.1016/s0162-0134(00)00046-5" @default.
• W1973688819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10885470" @default.
• W1973688819 hasPublicationYear "2000" @default.
• W1973688819 type Work @default.
• W1973688819 sameAs 1973688819 @default.
• W1973688819 citedByCount "34" @default.
• W1973688819 countsByYear W19736888192012 @default.
• W1973688819 countsByYear W19736888192013 @default.
• W1973688819 countsByYear W19736888192015 @default.
• W1973688819 countsByYear W19736888192016 @default.
• W1973688819 countsByYear W19736888192018 @default.
• W1973688819 countsByYear W19736888192020 @default.
• W1973688819 countsByYear W19736888192022 @default.
• W1973688819 crossrefType "journal-article" @default.
• W1973688819 hasAuthorship W1973688819A5001560379 @default.
• W1973688819 hasAuthorship W1973688819A5006229927 @default.
• W1973688819 hasAuthorship W1973688819A5038410313 @default.
• W1973688819 hasAuthorship W1973688819A5056671277 @default.
• W1973688819 hasAuthorship W1973688819A5091821876 @default.
• W1973688819 hasConcept C103408237 @default.
• W1973688819 hasConcept C104317684 @default.
• W1973688819 hasConcept C107824862 @default.
• W1973688819 hasConcept C116569031 @default.
• W1973688819 hasConcept C121332964 @default.
• W1973688819 hasConcept C143065580 @default.
• W1973688819 hasConcept C16318435 @default.
• W1973688819 hasConcept C170493617 @default.
• W1973688819 hasConcept C17165728 @default.
• W1973688819 hasConcept C179104552 @default.
• W1973688819 hasConcept C181199279 @default.
• W1973688819 hasConcept C185592680 @default.
• W1973688819 hasConcept C187961010 @default.
• W1973688819 hasConcept C28452662 @default.
• W1973688819 hasConcept C46141821 @default.
• W1973688819 hasConcept C501734568 @default.
• W1973688819 hasConcept C504678139 @default.
• W1973688819 hasConcept C55493867 @default.
• W1973688819 hasConcept C71240020 @default.
• W1973688819 hasConceptScore W1973688819C103408237 @default.
• W1973688819 hasConceptScore W1973688819C104317684 @default.
• W1973688819 hasConceptScore W1973688819C107824862 @default.
• W1973688819 hasConceptScore W1973688819C116569031 @default.
• W1973688819 hasConceptScore W1973688819C121332964 @default.
• W1973688819 hasConceptScore W1973688819C143065580 @default.
• W1973688819 hasConceptScore W1973688819C16318435 @default.
• W1973688819 hasConceptScore W1973688819C170493617 @default.
• W1973688819 hasConceptScore W1973688819C17165728 @default.
• W1973688819 hasConceptScore W1973688819C179104552 @default.
• W1973688819 hasConceptScore W1973688819C181199279 @default.
• W1973688819 hasConceptScore W1973688819C185592680 @default.
• W1973688819 hasConceptScore W1973688819C187961010 @default.

• next