FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973704454> ?p ?o ?g. }

• W1973704454 abstract "Chronic coactivation of alpha(2B)- and beta(2)-adrenoceptors (AR) was recently reported to down-regulate the alpha(2B)-AR at a lower threshold epinephrine (EPI) concentration compared with the activation of alpha(2B)-AR alone. This is the result of a modest beta(2)-AR-dependent up-regulation of G protein-coupled receptor kinase 3 (GRK3). In the present study, we determined that increasing GRK2 or GRK3 levels, independent of beta(2)-AR activation, decreases the EC(50) concentration for agonist-induced down-regulation of the alpha(2B)-AR using NG108 cells with or without overexpression (2- to 10-fold) of GRK2 or GRK3. In parental NG108 cells, the EC(50) concentration of EPI required for down-regulation of the alpha(2B)-AR is 30 microM. A 2- to 3-fold overexpression of GRK3 in NG108 cells, however, reduces the EC(50) to 0.2 microM (a 150-fold decrease), whereas a comparable overexpression of GRK2 reduces it to 1 microM (a 30-fold decrease). However, when GRK3 or GRK2 in NG108 cells are overexpressed 8- to 10-fold, the EC(50) concentration (0.02 microM EPI) for alpha(2B)-AR down-regulation is reduced 1000-fold. These data clearly suggest that a modest (2- to 3-fold) up-regulation of GRK3 is more effective at enhancing the sensitivity of alpha(2B)-AR to down-regulation after exposure to EPI than a modest up-regulation of GRK2, but that both GRK2 and GRK3 are equally effective at inducing alpha(2B)-AR down-regulation when up-regulated 8- to 10-fold. To our knowledge, this is the first report to systematically demonstrate that GRKs, particularly GRK3, play a pivotal role in modulating the agonist EC(50) concentration that down-regulates the alpha(2B)-AR and thus adds a new dimension to an already intricate signaling network." @default.
• W1973704454 created "2016-06-24" @default.
• W1973704454 creator A5015040305 @default.
• W1973704454 creator A5028316446 @default.
• W1973704454 creator A5058247048 @default.
• W1973704454 date "2004-09-29" @default.
• W1973704454 modified "2023-09-26" @default.
• W1973704454 title "Cellular G Protein-Coupled Receptor Kinase Levels Regulate Sensitivity of the α2B-Adrenergic Receptor to Undergo Agonist-Induced Down-Regulation" @default.
• W1973704454 cites W1413516056 @default.
• W1973704454 cites W1775749144 @default.
• W1973704454 cites W1966525626 @default.
• W1973704454 cites W1968674267 @default.
• W1973704454 cites W1970085880 @default.
• W1973704454 cites W1974505575 @default.
• W1973704454 cites W1977730768 @default.
• W1973704454 cites W1994847069 @default.
• W1973704454 cites W2001668793 @default.
• W1973704454 cites W2006910808 @default.
• W1973704454 cites W2020254877 @default.
• W1973704454 cites W2021635705 @default.
• W1973704454 cites W2025030241 @default.
• W1973704454 cites W2025472785 @default.
• W1973704454 cites W2028022490 @default.
• W1973704454 cites W2035577010 @default.
• W1973704454 cites W2047896617 @default.
• W1973704454 cites W2055375040 @default.
• W1973704454 cites W2067011931 @default.
• W1973704454 cites W2071445127 @default.
• W1973704454 cites W2073044318 @default.
• W1973704454 cites W2073404673 @default.
• W1973704454 cites W2074107470 @default.
• W1973704454 cites W2077746022 @default.
• W1973704454 cites W2132604666 @default.
• W1973704454 cites W2133354537 @default.
• W1973704454 cites W2133919483 @default.
• W1973704454 cites W2147710306 @default.
• W1973704454 cites W2179645574 @default.
• W1973704454 cites W2322465130 @default.
• W1973704454 cites W2419059488 @default.
• W1973704454 cites W4242669982 @default.
• W1973704454 doi "https://doi.org/10.1124/jpet.104.076042" @default.
• W1973704454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15456839" @default.
• W1973704454 hasPublicationYear "2004" @default.
• W1973704454 type Work @default.
• W1973704454 sameAs 1973704454 @default.
• W1973704454 citedByCount "10" @default.
• W1973704454 countsByYear W19737044542012 @default.
• W1973704454 countsByYear W19737044542016 @default.
• W1973704454 crossrefType "journal-article" @default.
• W1973704454 hasAuthorship W1973704454A5015040305 @default.
• W1973704454 hasAuthorship W1973704454A5028316446 @default.
• W1973704454 hasAuthorship W1973704454A5058247048 @default.
• W1973704454 hasConcept C104317684 @default.
• W1973704454 hasConcept C126322002 @default.
• W1973704454 hasConcept C127561419 @default.
• W1973704454 hasConcept C134018914 @default.
• W1973704454 hasConcept C135285700 @default.
• W1973704454 hasConcept C153911025 @default.
• W1973704454 hasConcept C170493617 @default.
• W1973704454 hasConcept C184235292 @default.
• W1973704454 hasConcept C185592680 @default.
• W1973704454 hasConcept C202751555 @default.
• W1973704454 hasConcept C2778938600 @default.
• W1973704454 hasConcept C33235085 @default.
• W1973704454 hasConcept C55493867 @default.
• W1973704454 hasConcept C57074206 @default.
• W1973704454 hasConcept C71924100 @default.
• W1973704454 hasConcept C78976303 @default.
• W1973704454 hasConcept C86803240 @default.
• W1973704454 hasConcept C95444343 @default.
• W1973704454 hasConcept C96529984 @default.
• W1973704454 hasConceptScore W1973704454C104317684 @default.
• W1973704454 hasConceptScore W1973704454C126322002 @default.
• W1973704454 hasConceptScore W1973704454C127561419 @default.
• W1973704454 hasConceptScore W1973704454C134018914 @default.
• W1973704454 hasConceptScore W1973704454C135285700 @default.
• W1973704454 hasConceptScore W1973704454C153911025 @default.
• W1973704454 hasConceptScore W1973704454C170493617 @default.
• W1973704454 hasConceptScore W1973704454C184235292 @default.
• W1973704454 hasConceptScore W1973704454C185592680 @default.
• W1973704454 hasConceptScore W1973704454C202751555 @default.
• W1973704454 hasConceptScore W1973704454C2778938600 @default.
• W1973704454 hasConceptScore W1973704454C33235085 @default.
• W1973704454 hasConceptScore W1973704454C55493867 @default.
• W1973704454 hasConceptScore W1973704454C57074206 @default.
• W1973704454 hasConceptScore W1973704454C71924100 @default.
• W1973704454 hasConceptScore W1973704454C78976303 @default.
• W1973704454 hasConceptScore W1973704454C86803240 @default.
• W1973704454 hasConceptScore W1973704454C95444343 @default.
• W1973704454 hasConceptScore W1973704454C96529984 @default.
• W1973704454 hasLocation W19737044541 @default.
• W1973704454 hasLocation W19737044542 @default.
• W1973704454 hasOpenAccess W1973704454 @default.
• W1973704454 hasPrimaryLocation W19737044541 @default.
• W1973704454 hasRelatedWork W1970287328 @default.
• W1973704454 hasRelatedWork W2027913950 @default.
• W1973704454 hasRelatedWork W2034583712 @default.
• W1973704454 hasRelatedWork W2050423574 @default.
• W1973704454 hasRelatedWork W2058205365 @default.
• W1973704454 hasRelatedWork W2074821979 @default.

• next