Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973732701> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1973732701 endingPage "110" @default.
- W1973732701 startingPage "103" @default.
- W1973732701 abstract "Bacillus subtilis synthesizes the lanthionine containing 32-amino-acid peptide antibiotic (lanti-biotic) subtilin from a ribosomally generated 56-amino-acid precursor pre-propeptide by extensive posttranslational modifications. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used to monitor the production of matured subtilin within crude samples taken from B. subtilis culture media without prior fractionation. The processing reaction of subtilin was blocked with the serine protease inhibitor phenylmethylsulfonyl fluoride and different subtilin precursor peptides in the molecular mass range up to 6220 were observed. Two of these species were isolated by reversed-phase high-performance liquid chromatography (HPLC) and structurally analyzed by post-source decay MALDI-TOFMS. We provide evidence that the precursor species comprise the posttranslational modified C-terminal part of subtilin to which leader peptide moieties with different chain lengths are attached. These antimicrobial-inactive species could be processed to antibiotic-active subtilin by incubation with culture media of different subtilin-nonproducing B. subtilis strains as indicated by a combination of antimicrobial growth assays and MALDI-TOFMS analyses. These achievements are strong evidence for the sensitivity of MALDI-TOFMS methodology that allows straightforward investigations of analytes even in complex mixtures without time-consuming sample preparations." @default.
- W1973732701 created "2016-06-24" @default.
- W1973732701 creator A5017832179 @default.
- W1973732701 creator A5068197565 @default.
- W1973732701 date "2001-12-13" @default.
- W1973732701 modified "2023-10-09" @default.
- W1973732701 title "Maturation of the lantibiotic subtilin: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to monitor precursors and their proteolytic processing in crude bacterial cultures" @default.
- W1973732701 cites W1480208536 @default.
- W1973732701 cites W1517536140 @default.
- W1973732701 cites W1570137458 @default.
- W1973732701 cites W1853858368 @default.
- W1973732701 cites W1916193181 @default.
- W1973732701 cites W1961587862 @default.
- W1973732701 cites W1963680299 @default.
- W1973732701 cites W1967100339 @default.
- W1973732701 cites W1984220817 @default.
- W1973732701 cites W1984233997 @default.
- W1973732701 cites W1997325165 @default.
- W1973732701 cites W2005589295 @default.
- W1973732701 cites W2006225651 @default.
- W1973732701 cites W2008912752 @default.
- W1973732701 cites W2011781149 @default.
- W1973732701 cites W2051628700 @default.
- W1973732701 cites W2093962701 @default.
- W1973732701 cites W2095830437 @default.
- W1973732701 cites W2100882223 @default.
- W1973732701 cites W2119569975 @default.
- W1973732701 cites W2147954340 @default.
- W1973732701 cites W2156133722 @default.
- W1973732701 cites W2048628617 @default.
- W1973732701 doi "https://doi.org/10.1002/rcm.552" @default.
- W1973732701 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11754255" @default.
- W1973732701 hasPublicationYear "2001" @default.
- W1973732701 type Work @default.
- W1973732701 sameAs 1973732701 @default.
- W1973732701 citedByCount "32" @default.
- W1973732701 countsByYear W19737327012013 @default.
- W1973732701 countsByYear W19737327012014 @default.
- W1973732701 countsByYear W19737327012016 @default.
- W1973732701 countsByYear W19737327012017 @default.
- W1973732701 countsByYear W19737327012019 @default.
- W1973732701 countsByYear W19737327012020 @default.
- W1973732701 countsByYear W19737327012022 @default.
- W1973732701 crossrefType "journal-article" @default.
- W1973732701 hasAuthorship W1973732701A5017832179 @default.
- W1973732701 hasAuthorship W1973732701A5068197565 @default.
- W1973732701 hasConcept C132141352 @default.
- W1973732701 hasConcept C150394285 @default.
- W1973732701 hasConcept C162356407 @default.
- W1973732701 hasConcept C162711632 @default.
- W1973732701 hasConcept C178790620 @default.
- W1973732701 hasConcept C185592680 @default.
- W1973732701 hasConcept C2777272437 @default.
- W1973732701 hasConcept C2778457984 @default.
- W1973732701 hasConcept C2779281246 @default.
- W1973732701 hasConcept C43617362 @default.
- W1973732701 hasConcept C4937899 @default.
- W1973732701 hasConcept C523546767 @default.
- W1973732701 hasConcept C54355233 @default.
- W1973732701 hasConcept C55493867 @default.
- W1973732701 hasConcept C75280812 @default.
- W1973732701 hasConcept C86803240 @default.
- W1973732701 hasConceptScore W1973732701C132141352 @default.
- W1973732701 hasConceptScore W1973732701C150394285 @default.
- W1973732701 hasConceptScore W1973732701C162356407 @default.
- W1973732701 hasConceptScore W1973732701C162711632 @default.
- W1973732701 hasConceptScore W1973732701C178790620 @default.
- W1973732701 hasConceptScore W1973732701C185592680 @default.
- W1973732701 hasConceptScore W1973732701C2777272437 @default.
- W1973732701 hasConceptScore W1973732701C2778457984 @default.
- W1973732701 hasConceptScore W1973732701C2779281246 @default.
- W1973732701 hasConceptScore W1973732701C43617362 @default.
- W1973732701 hasConceptScore W1973732701C4937899 @default.
- W1973732701 hasConceptScore W1973732701C523546767 @default.
- W1973732701 hasConceptScore W1973732701C54355233 @default.
- W1973732701 hasConceptScore W1973732701C55493867 @default.
- W1973732701 hasConceptScore W1973732701C75280812 @default.
- W1973732701 hasConceptScore W1973732701C86803240 @default.
- W1973732701 hasIssue "2" @default.
- W1973732701 hasLocation W19737327011 @default.
- W1973732701 hasLocation W19737327012 @default.
- W1973732701 hasOpenAccess W1973732701 @default.
- W1973732701 hasPrimaryLocation W19737327011 @default.
- W1973732701 hasRelatedWork W1828402102 @default.
- W1973732701 hasRelatedWork W1971203269 @default.
- W1973732701 hasRelatedWork W2066458926 @default.
- W1973732701 hasRelatedWork W2090464384 @default.
- W1973732701 hasRelatedWork W2163834922 @default.
- W1973732701 hasRelatedWork W2327863492 @default.
- W1973732701 hasRelatedWork W2885667616 @default.
- W1973732701 hasRelatedWork W2904410259 @default.
- W1973732701 hasRelatedWork W2912934522 @default.
- W1973732701 hasRelatedWork W2952412205 @default.
- W1973732701 hasVolume "16" @default.
- W1973732701 isParatext "false" @default.
- W1973732701 isRetracted "false" @default.
- W1973732701 magId "1973732701" @default.
- W1973732701 workType "article" @default.