Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973737269> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1973737269 endingPage "7677" @default.
- W1973737269 startingPage "7670" @default.
- W1973737269 abstract "Beta-Arg-182 in Escherichia coli F1-ATPase (beta-Arg-189 in bovine mitochondrial F1) is a residue which lies close to catalytic site bound nucleotide (Abrahams et al. (1994) Nature 370, 621-628). Here we investigated the role of this residue by characterizing two mutants, betaR182Q and betaR182K. Oxidative phosphorylation and steady-state ATPase activity of purified F1 were severely impaired by both mutations. Catalytic site nucleotide-binding parameters were measured using the fluorescence quench of beta-Trp-331 that occurred upon nucleotide binding to purified F1 from betaR182Q/betaY331W and betaR182K/betaY331W double mutants. It was found that (a) beta-Arg-182 interacts with the gamma-phosphate of MgATP, particularly at catalytic sites 1 and 2, (b) beta-Arg-182 has no functional interaction with the beta-phosphate of MgADP or with the magnesium of the magnesium-nucleotide complex in the catalytic sites, and (c) beta-Arg-182 is directly involved in the stabilization of the catalytic transition state. In these features the role of beta-Arg-182 resembles that of another positively charged residue in the catalytic site, the conserved lysine of the Walker A motif, beta-Lys-155. A further role of beta-Arg-182 is suggested, namely involvement in conformational change at the catalytic site beta-alpha subunit interface that is required for multisite catalysis." @default.
- W1973737269 created "2016-06-24" @default.
- W1973737269 creator A5058095118 @default.
- W1973737269 creator A5060832624 @default.
- W1973737269 creator A5073833369 @default.
- W1973737269 date "1999-05-26" @default.
- W1973737269 modified "2023-09-27" @default.
- W1973737269 title "The Role of β-Arg-182, an Essential Catalytic Site Residue in <i>Escherichia coli</i> F<sub>1</sub>-ATPase" @default.
- W1973737269 cites W1523855184 @default.
- W1973737269 cites W1584144567 @default.
- W1973737269 cites W1607491058 @default.
- W1973737269 cites W1795789692 @default.
- W1973737269 cites W1984555522 @default.
- W1973737269 cites W1994450639 @default.
- W1973737269 cites W2033202752 @default.
- W1973737269 cites W2038063003 @default.
- W1973737269 cites W2042805323 @default.
- W1973737269 cites W2090862274 @default.
- W1973737269 cites W2156435290 @default.
- W1973737269 doi "https://doi.org/10.1021/bi990663x" @default.
- W1973737269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10387006" @default.
- W1973737269 hasPublicationYear "1999" @default.
- W1973737269 type Work @default.
- W1973737269 sameAs 1973737269 @default.
- W1973737269 citedByCount "34" @default.
- W1973737269 countsByYear W19737372692012 @default.
- W1973737269 countsByYear W19737372692013 @default.
- W1973737269 crossrefType "journal-article" @default.
- W1973737269 hasAuthorship W1973737269A5058095118 @default.
- W1973737269 hasAuthorship W1973737269A5060832624 @default.
- W1973737269 hasAuthorship W1973737269A5073833369 @default.
- W1973737269 hasConcept C104292427 @default.
- W1973737269 hasConcept C104317684 @default.
- W1973737269 hasConcept C107824862 @default.
- W1973737269 hasConcept C143065580 @default.
- W1973737269 hasConcept C161790260 @default.
- W1973737269 hasConcept C181199279 @default.
- W1973737269 hasConcept C185592680 @default.
- W1973737269 hasConcept C23265538 @default.
- W1973737269 hasConcept C2777339483 @default.
- W1973737269 hasConcept C2781338088 @default.
- W1973737269 hasConcept C41183919 @default.
- W1973737269 hasConcept C512185932 @default.
- W1973737269 hasConcept C547475151 @default.
- W1973737269 hasConcept C55493867 @default.
- W1973737269 hasConcept C71240020 @default.
- W1973737269 hasConceptScore W1973737269C104292427 @default.
- W1973737269 hasConceptScore W1973737269C104317684 @default.
- W1973737269 hasConceptScore W1973737269C107824862 @default.
- W1973737269 hasConceptScore W1973737269C143065580 @default.
- W1973737269 hasConceptScore W1973737269C161790260 @default.
- W1973737269 hasConceptScore W1973737269C181199279 @default.
- W1973737269 hasConceptScore W1973737269C185592680 @default.
- W1973737269 hasConceptScore W1973737269C23265538 @default.
- W1973737269 hasConceptScore W1973737269C2777339483 @default.
- W1973737269 hasConceptScore W1973737269C2781338088 @default.
- W1973737269 hasConceptScore W1973737269C41183919 @default.
- W1973737269 hasConceptScore W1973737269C512185932 @default.
- W1973737269 hasConceptScore W1973737269C547475151 @default.
- W1973737269 hasConceptScore W1973737269C55493867 @default.
- W1973737269 hasConceptScore W1973737269C71240020 @default.
- W1973737269 hasIssue "24" @default.
- W1973737269 hasLocation W19737372691 @default.
- W1973737269 hasLocation W19737372692 @default.
- W1973737269 hasOpenAccess W1973737269 @default.
- W1973737269 hasPrimaryLocation W19737372691 @default.
- W1973737269 hasRelatedWork W1516725542 @default.
- W1973737269 hasRelatedWork W1518303236 @default.
- W1973737269 hasRelatedWork W1595435363 @default.
- W1973737269 hasRelatedWork W1826029693 @default.
- W1973737269 hasRelatedWork W1972665833 @default.
- W1973737269 hasRelatedWork W2007545289 @default.
- W1973737269 hasRelatedWork W2065679107 @default.
- W1973737269 hasRelatedWork W2067448645 @default.
- W1973737269 hasRelatedWork W2139244796 @default.
- W1973737269 hasRelatedWork W258580054 @default.
- W1973737269 hasVolume "38" @default.
- W1973737269 isParatext "false" @default.
- W1973737269 isRetracted "false" @default.
- W1973737269 magId "1973737269" @default.
- W1973737269 workType "article" @default.