FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973804925> ?p ?o ?g. }

• W1973804925 endingPage "041210" @default.
• W1973804925 startingPage "041210" @default.
• W1973804925 abstract "In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation. Understanding this relationship is essential for detection and quantification of signal. We have studied four codon optimized luciferases with different emission spectra, including enzymes from firefly (FLuc), click beetle (CBGr68, CBRed) and Renilla reniformins (hRLuc). At 25 degrees C, the in vitro lambda(max) of these reporters are 578, 543, 615, and 480 nm, respectively; at body temperature, 37 degrees C, the brightness increases and the firefly enzyme demonstrates a 34-nm spectral red shift. Spectral shifts and attenuation due to tissue effects were evaluated using a series of 20-nm bandpass filters and a cooled charge-coupled device (CCD) camera. Attenuation increased and the spectra of emitted light was red shifted for signals originating from deeper within the body relative to superficial origins. The tissue attenuation of signals from CBGr68 and hRLuc was greater than from those of Fluc and CBRed. To further probe tissue effects, broad spectral emitters were created through gene fusions between CBGr68 and CBRed. These resulted in enzymes with broader emission spectra, featuring two peaks whose intensities are differentially affected by temperature and tissue depth. These spectral measurement data allow for improved understanding of how these reporters can be used in vivo and what they can reveal about biological processes in living subjects." @default.
• W1973804925 created "2016-06-24" @default.
• W1973804925 creator A5008116183 @default.
• W1973804925 creator A5019700261 @default.
• W1973804925 creator A5022780080 @default.
• W1973804925 creator A5024646275 @default.
• W1973804925 creator A5079277904 @default.
• W1973804925 creator A5081671050 @default.
• W1973804925 date "2005-01-01" @default.
• W1973804925 modified "2023-10-02" @default.
• W1973804925 title "Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo" @default.
• W1973804925 cites W1607922172 @default.
• W1973804925 cites W1620954807 @default.
• W1973804925 cites W1968603674 @default.
• W1973804925 cites W1985316610 @default.
• W1973804925 cites W1996666817 @default.
• W1973804925 cites W2011261569 @default.
• W1973804925 cites W2020202068 @default.
• W1973804925 cites W2035074260 @default.
• W1973804925 cites W2055398497 @default.
• W1973804925 cites W2056817309 @default.
• W1973804925 cites W2063119860 @default.
• W1973804925 cites W2068408712 @default.
• W1973804925 cites W2117419072 @default.
• W1973804925 cites W2118712112 @default.
• W1973804925 cites W2144751606 @default.
• W1973804925 cites W2152439945 @default.
• W1973804925 cites W2170405846 @default.
• W1973804925 cites W2172643117 @default.
• W1973804925 cites W2320147764 @default.
• W1973804925 cites W4233878159 @default.
• W1973804925 doi "https://doi.org/10.1117/1.2032388" @default.
• W1973804925 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16178634" @default.
• W1973804925 hasPublicationYear "2005" @default.
• W1973804925 type Work @default.
• W1973804925 sameAs 1973804925 @default.
• W1973804925 citedByCount "288" @default.
• W1973804925 countsByYear W19738049252012 @default.
• W1973804925 countsByYear W19738049252013 @default.
• W1973804925 countsByYear W19738049252014 @default.
• W1973804925 countsByYear W19738049252015 @default.
• W1973804925 countsByYear W19738049252016 @default.
• W1973804925 countsByYear W19738049252017 @default.
• W1973804925 countsByYear W19738049252018 @default.
• W1973804925 countsByYear W19738049252019 @default.
• W1973804925 countsByYear W19738049252020 @default.
• W1973804925 countsByYear W19738049252021 @default.
• W1973804925 countsByYear W19738049252022 @default.
• W1973804925 countsByYear W19738049252023 @default.
• W1973804925 crossrefType "journal-article" @default.
• W1973804925 hasAuthorship W1973804925A5008116183 @default.
• W1973804925 hasAuthorship W1973804925A5019700261 @default.
• W1973804925 hasAuthorship W1973804925A5022780080 @default.
• W1973804925 hasAuthorship W1973804925A5024646275 @default.
• W1973804925 hasAuthorship W1973804925A5079277904 @default.
• W1973804925 hasAuthorship W1973804925A5081671050 @default.
• W1973804925 hasBestOaLocation W19738049251 @default.
• W1973804925 hasConcept C104317684 @default.
• W1973804925 hasConcept C111335760 @default.
• W1973804925 hasConcept C120665830 @default.
• W1973804925 hasConcept C121332964 @default.
• W1973804925 hasConcept C12554922 @default.
• W1973804925 hasConcept C1276947 @default.
• W1973804925 hasConcept C184652730 @default.
• W1973804925 hasConcept C185592680 @default.
• W1973804925 hasConcept C192562407 @default.
• W1973804925 hasConcept C207001950 @default.
• W1973804925 hasConcept C2909536089 @default.
• W1973804925 hasConcept C46141821 @default.
• W1973804925 hasConcept C4839761 @default.
• W1973804925 hasConcept C5176359 @default.
• W1973804925 hasConcept C54009773 @default.
• W1973804925 hasConcept C54355233 @default.
• W1973804925 hasConcept C55493867 @default.
• W1973804925 hasConcept C86803240 @default.
• W1973804925 hasConcept C96141758 @default.
• W1973804925 hasConceptScore W1973804925C104317684 @default.
• W1973804925 hasConceptScore W1973804925C111335760 @default.
• W1973804925 hasConceptScore W1973804925C120665830 @default.
• W1973804925 hasConceptScore W1973804925C121332964 @default.
• W1973804925 hasConceptScore W1973804925C12554922 @default.
• W1973804925 hasConceptScore W1973804925C1276947 @default.
• W1973804925 hasConceptScore W1973804925C184652730 @default.
• W1973804925 hasConceptScore W1973804925C185592680 @default.
• W1973804925 hasConceptScore W1973804925C192562407 @default.
• W1973804925 hasConceptScore W1973804925C207001950 @default.
• W1973804925 hasConceptScore W1973804925C2909536089 @default.
• W1973804925 hasConceptScore W1973804925C46141821 @default.
• W1973804925 hasConceptScore W1973804925C4839761 @default.
• W1973804925 hasConceptScore W1973804925C5176359 @default.
• W1973804925 hasConceptScore W1973804925C54009773 @default.
• W1973804925 hasConceptScore W1973804925C54355233 @default.
• W1973804925 hasConceptScore W1973804925C55493867 @default.
• W1973804925 hasConceptScore W1973804925C86803240 @default.
• W1973804925 hasConceptScore W1973804925C96141758 @default.
• W1973804925 hasIssue "4" @default.
• W1973804925 hasLocation W19738049251 @default.
• W1973804925 hasLocation W19738049252 @default.

• next