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- W1973818768 abstract "We have built a fluorescence correlation spectroscopy (FCS) microscope for ultraviolet excitation (280-300 nm) and emission. With UV excitation the fluorescence of 'natural fluorophores' such as the modified nucleotide 2-aminopurine can be analyzed. The sensitivity of a natural fluorophore toward conformational changes can reveal dynamics in biomolecules. UV-FCS is well suited for detection of intensity fluctuations related to such conformational dynamics. Here we show UV-FCS measured on p-Quarterphenyl and on 2-aminopurine (2-AP). The triplet state rate constants and the excitation cross section for 2-AP were estimated to k23 = 1 x 10(6) s(-1), k31 = 3 x 10(5) s(-1), and sigma(exc) = 2 x 10(-17) cm2." @default.
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- W1973818768 date "2001-01-21" @default.
- W1973818768 modified "2023-10-16" @default.
- W1973818768 title "UV-Fluorescence Correlation Spectroscopy of 2-Aminopurine" @default.
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- W1973818768 doi "https://doi.org/10.1515/bc.2001.048" @default.
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