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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973855660> ?p ?o ?g. }

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• W1973855660 abstract "Condensation of dimeric 3,4,6-tri-O-acetyl-2-deoxy-2-nitroso-α-d-glucopyranosyl chloride with 4-methylumbelliferone gave crystalline 4-methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-oximino-α-d-arabino-hexopyranoside. Acetylation of this adduct, reduction of the resulting crude O-acetyloxime with borane in oxolane, and acetylation gave the 3,4,6-tri-O-acetyl derivative of 4-methylumbelliferyl 2-acetamido-2-deoxy-α-d-glucopyranoside (1). A new sensitive assay of N-acetyl-α-d-glucos-aminidase (EC 3.2.1.50) is made possible by fluorometric measurement of 4-methyl-umbelliferone liberated by enzymic hydrolysis of glycoside 1. Such assays are illustrated by results obtained with enzyme preparations from pig liver and human-blood serum." @default.
• W1973855660 created "2016-06-24" @default.
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• W1973855660 date "1981-10-01" @default.
• W1973855660 modified "2023-10-16" @default.
• W1973855660 title "4-Methylumbelliferyl 2-acetamido-2-deoxy-α-d-glucopyranoside, a fluorogenic substrate for N-acetyl-α-D-glucos-aminidase" @default.
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• W1973855660 doi "https://doi.org/10.1016/s0008-6215(00)84698-4" @default.
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