FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973876528> ?p ?o ?g. }

• W1973876528 endingPage "129" @default.
• W1973876528 startingPage "116" @default.
• W1973876528 abstract "1. Upon incubation for 1 h at 37°C, proenzymic C1r was activated by a proteolytic cleavage comparable to that observed in vivo; after reduction and alkylation, two fragments of apparent molecular weights 57 000 and 35 000 were evident on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The activation kinetics were slightly sigmoidal and nearly independent of C1r concentration. They were characterized by a marked thermal dependence (activation energy = 45 kcal/mol). The reaction was inhibited by calcium and p-nitrophenyl-p′-guanidinobenzoate, but poorly sensitive to di-isopropyl phosphorofluoridate. The dependence of the activation rate on pH was unusual; it decreased progressively in the acid range (pH 4.5–6.5) which coincides with the dissociation of the C1r-C1r dimer. Above pH 6.5, the rate increased slightly and showed no clear maximum. These results are consistent with an intramolecular autocatalytic activation mechanism involving the prosite of each subunit of the C1r-C1r dimer. 2. During a 5 h incubation period at 37°C, C1r underwent two proteolytic cleavages which led to the successive removal of two fragments, α (35 000) and β (7 000–11 000) from each subunit, leaving a dimeric molecule of reduced size (Mr = 110 000; s20,w = 6.1 S). The proteolytic process was nearly independent of C1r concentration and characterized by a pH optimum at 8.5–9.0, and a high activation energy (36.8 kcal/mol). Calcium and p-nitrophenyl-p′-guanidinobenzoate, and also di-isopropyl phosphorofluoridate and benzamidine were inhibitors of this reaction. The product, C1r II, retained the original antigenic properties of C1r and a functional active site, but lost the capacity to bind C1r. These results are consistent with an autocatalytic intramolecular proteolysis mediated by the active site of each subunit of the C1r-C1r dimer." @default.
• W1973876528 created "2016-06-24" @default.
• W1973876528 creator A5018858147 @default.
• W1973876528 creator A5039426717 @default.
• W1973876528 creator A5040271205 @default.
• W1973876528 creator A5058438688 @default.
• W1973876528 date "1980-11-01" @default.
• W1973876528 modified "2023-10-15" @default.
• W1973876528 title "Purified proenzyme C1r. Some characteristics of its activation and subsequent proteolytic cleavage" @default.
• W1973876528 cites W134038162 @default.
• W1973876528 cites W1460551420 @default.
• W1973876528 cites W1485267531 @default.
• W1973876528 cites W1543676951 @default.
• W1973876528 cites W1584597846 @default.
• W1973876528 cites W1586723771 @default.
• W1973876528 cites W1625660792 @default.
• W1973876528 cites W1977909365 @default.
• W1973876528 cites W1983623773 @default.
• W1973876528 cites W1989903975 @default.
• W1973876528 cites W2005102218 @default.
• W1973876528 cites W2024480784 @default.
• W1973876528 cites W2045044378 @default.
• W1973876528 cites W2057462192 @default.
• W1973876528 cites W2063415795 @default.
• W1973876528 cites W2066889294 @default.
• W1973876528 cites W2081251979 @default.
• W1973876528 cites W2128390802 @default.
• W1973876528 cites W2143954959 @default.
• W1973876528 cites W2273370733 @default.
• W1973876528 cites W2403416872 @default.
• W1973876528 cites W2465892129 @default.
• W1973876528 cites W99373296 @default.
• W1973876528 doi "https://doi.org/10.1016/0005-2744(80)90269-7" @default.
• W1973876528 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6254570" @default.
• W1973876528 hasPublicationYear "1980" @default.
• W1973876528 type Work @default.
• W1973876528 sameAs 1973876528 @default.
• W1973876528 citedByCount "84" @default.
• W1973876528 countsByYear W19738765282012 @default.
• W1973876528 countsByYear W19738765282013 @default.
• W1973876528 countsByYear W19738765282016 @default.
• W1973876528 countsByYear W19738765282017 @default.
• W1973876528 countsByYear W19738765282019 @default.
• W1973876528 crossrefType "journal-article" @default.
• W1973876528 hasAuthorship W1973876528A5018858147 @default.
• W1973876528 hasAuthorship W1973876528A5039426717 @default.
• W1973876528 hasAuthorship W1973876528A5040271205 @default.
• W1973876528 hasAuthorship W1973876528A5058438688 @default.
• W1973876528 hasConcept C121332964 @default.
• W1973876528 hasConcept C13965031 @default.
• W1973876528 hasConcept C148898269 @default.
• W1973876528 hasConcept C151730666 @default.
• W1973876528 hasConcept C155647269 @default.
• W1973876528 hasConcept C161790260 @default.
• W1973876528 hasConcept C175156509 @default.
• W1973876528 hasConcept C178790620 @default.
• W1973876528 hasConcept C181199279 @default.
• W1973876528 hasConcept C185592680 @default.
• W1973876528 hasConcept C2779546866 @default.
• W1973876528 hasConcept C3019223276 @default.
• W1973876528 hasConcept C37263863 @default.
• W1973876528 hasConcept C43369102 @default.
• W1973876528 hasConcept C55493867 @default.
• W1973876528 hasConcept C62520636 @default.
• W1973876528 hasConcept C71240020 @default.
• W1973876528 hasConcept C86803240 @default.
• W1973876528 hasConcept C95121573 @default.
• W1973876528 hasConceptScore W1973876528C121332964 @default.
• W1973876528 hasConceptScore W1973876528C13965031 @default.
• W1973876528 hasConceptScore W1973876528C148898269 @default.
• W1973876528 hasConceptScore W1973876528C151730666 @default.
• W1973876528 hasConceptScore W1973876528C155647269 @default.
• W1973876528 hasConceptScore W1973876528C161790260 @default.
• W1973876528 hasConceptScore W1973876528C175156509 @default.
• W1973876528 hasConceptScore W1973876528C178790620 @default.
• W1973876528 hasConceptScore W1973876528C181199279 @default.
• W1973876528 hasConceptScore W1973876528C185592680 @default.
• W1973876528 hasConceptScore W1973876528C2779546866 @default.
• W1973876528 hasConceptScore W1973876528C3019223276 @default.
• W1973876528 hasConceptScore W1973876528C37263863 @default.
• W1973876528 hasConceptScore W1973876528C43369102 @default.
• W1973876528 hasConceptScore W1973876528C55493867 @default.
• W1973876528 hasConceptScore W1973876528C62520636 @default.
• W1973876528 hasConceptScore W1973876528C71240020 @default.
• W1973876528 hasConceptScore W1973876528C86803240 @default.
• W1973876528 hasConceptScore W1973876528C95121573 @default.
• W1973876528 hasIssue "1" @default.
• W1973876528 hasLocation W19738765281 @default.
• W1973876528 hasLocation W19738765282 @default.
• W1973876528 hasOpenAccess W1973876528 @default.
• W1973876528 hasPrimaryLocation W19738765281 @default.
• W1973876528 hasRelatedWork W2033712307 @default.
• W1973876528 hasRelatedWork W2036886809 @default.
• W1973876528 hasRelatedWork W2123143003 @default.
• W1973876528 hasRelatedWork W2150938652 @default.
• W1973876528 hasRelatedWork W2357322967 @default.
• W1973876528 hasRelatedWork W2612513854 @default.
• W1973876528 hasRelatedWork W2900854427 @default.

• next