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• W1973919030 abstract "For many years anabolic-androgenic steroids (AAS) are by far the most frequently detected pharmacological substances in doping control. In order to improve their performances, professional sportsmen are often tempted to take dietary supplements. However, due to the frequent and widespread occurrence of contaminated supplements, the use of such products is not without risk for the athletes involved. In order to minimize the chances of an unattended positive doping test or serious health problems, fast and reliable screening methods for the detection of anabolic steroids in dietary supplements are needed. A general screening procedure requires the fast and unambiguous detection of a large range of steroids. Gas chromatography–mass spectrometry (GC–MS) has been used intensively in the detection of doping substances for the past 40 years. Over time, many laboratories have delivered spectra to be included in standard reference databases, one of which is maintained by the National Institute of Standards and Technology (NIST) (Gaithersburg, MD, USA). In recent years, however, liquid chromatography coupled to mass spectrometry (LC–MS) has gained popularity. Unfortunately, existing GC–MS libraries are not applicable to LC–MS analysis. In the present study, a new mass spectral library of 88 steroids was developed, along with a fast UPLC–MS method. For the construction of this mass spectral library, three different mass spectra were measured for each steroid, with a sample cone voltage of 30, 60 and 100 V, respectively. This method was then successfully tested on contaminated dietary supplements which had previously been tested by means of a targeted LC–MS/MS method. Overall, the library search was shown to identify the same compounds as the MRM method. Copyright © 2011 John Wiley & Sons, Ltd." @default.
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• W1973919030 date "2011-03-01" @default.
• W1973919030 modified "2023-09-27" @default.
• W1973919030 title "An LC-MS screening method with library identification for the detection of steroidsin dietary supplements" @default.
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• W1973919030 doi "https://doi.org/10.1002/jms.1899" @default.
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