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- W1973928813 abstract "Somatic embryogenesis of Calamus manan, a single-stemmed rattan species, in tissue culture was scientifically demonstrated for the first time. Root tips of in vitro plantlets produced friable callus when the explants were cultivated for several mo. on a Murashige and Skoog induction medium containing 7.5 mg Picloram per l (31.1 µM). Histological analyses established the presence of proembryos within the callus which differentiated subsequently into somatic embryos using the same culture medium. Histological examination revealed that these somatic embryos completely lacked starch and protein reserves, which did not prevent them, however, from germinating, and showing bipolar development. These somatic embryos further developed into young plants, similarly to zygotic embryos." @default.
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- W1973928813 date "1999-09-01" @default.
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- W1973928813 title "Evidence of somatic embryogenesis from root tip explants of the rattan Calamus manan" @default.
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- W1973928813 doi "https://doi.org/10.1007/s11627-999-0060-7" @default.
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