Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973946986> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1973946986 endingPage "385a" @default.
- W1973946986 startingPage "385a" @default.
- W1973946986 abstract "Gene expression is inherently stochastic, yet must be tightly regulated to properly carry out essential cellular processes. However, many of the transcription factors responsible for this precise regulation are expressed at extremely low copy numbers in cells. To understand how noise in gene expression is controlled to overcome this challenge, it is important to directly monitor gene regulation in real time at the single-molecule level. In this work, we use the genetic switch of lambda phage as a model system to demonstrate continuous monitoring of autoregulation of lambda repressor CI in living E. coli cells. CI exhibits both positive and negative feedback on its own expression at low and high intracellular concentrations, respectively. We generated a single-molecule, gene-expression reporter that enables counting of the exact number of CI molecules expressed in real time without compromising CI's regulatory activity. Over several cell generations, we observed highly clustered expression of CI separated by relatively long periods of low expression, which we attribute to the alternating positive and negative autoregulation of CI. Our results suggest that the noise in CI expression is controlled by coupled positive and negative feedback, a mechanism also implicated in regulation of the eukaryotic cell cycle and circadian rhythm. This method should be applicable to similar studies probing gene regulation in other systems." @default.
- W1973946986 created "2016-06-24" @default.
- W1973946986 creator A5003238937 @default.
- W1973946986 creator A5045471152 @default.
- W1973946986 creator A5083396495 @default.
- W1973946986 date "2009-02-01" @default.
- W1973946986 modified "2023-09-27" @default.
- W1973946986 title "A Single-Molecule Study of Gene Regulation in Real Time" @default.
- W1973946986 doi "https://doi.org/10.1016/j.bpj.2008.12.2883" @default.
- W1973946986 hasPublicationYear "2009" @default.
- W1973946986 type Work @default.
- W1973946986 sameAs 1973946986 @default.
- W1973946986 citedByCount "0" @default.
- W1973946986 crossrefType "journal-article" @default.
- W1973946986 hasAuthorship W1973946986A5003238937 @default.
- W1973946986 hasAuthorship W1973946986A5045471152 @default.
- W1973946986 hasAuthorship W1973946986A5083396495 @default.
- W1973946986 hasBestOaLocation W19739469861 @default.
- W1973946986 hasConcept C104317684 @default.
- W1973946986 hasConcept C111774379 @default.
- W1973946986 hasConcept C119599485 @default.
- W1973946986 hasConcept C121332964 @default.
- W1973946986 hasConcept C127413603 @default.
- W1973946986 hasConcept C134018914 @default.
- W1973946986 hasConcept C138885662 @default.
- W1973946986 hasConcept C150194340 @default.
- W1973946986 hasConcept C158448853 @default.
- W1973946986 hasConcept C165801399 @default.
- W1973946986 hasConcept C165864922 @default.
- W1973946986 hasConcept C179926584 @default.
- W1973946986 hasConcept C27153228 @default.
- W1973946986 hasConcept C41895202 @default.
- W1973946986 hasConcept C48207687 @default.
- W1973946986 hasConcept C54355233 @default.
- W1973946986 hasConcept C62520636 @default.
- W1973946986 hasConcept C70721500 @default.
- W1973946986 hasConcept C84393581 @default.
- W1973946986 hasConcept C86339819 @default.
- W1973946986 hasConcept C86803240 @default.
- W1973946986 hasConcept C93586867 @default.
- W1973946986 hasConcept C95444343 @default.
- W1973946986 hasConceptScore W1973946986C104317684 @default.
- W1973946986 hasConceptScore W1973946986C111774379 @default.
- W1973946986 hasConceptScore W1973946986C119599485 @default.
- W1973946986 hasConceptScore W1973946986C121332964 @default.
- W1973946986 hasConceptScore W1973946986C127413603 @default.
- W1973946986 hasConceptScore W1973946986C134018914 @default.
- W1973946986 hasConceptScore W1973946986C138885662 @default.
- W1973946986 hasConceptScore W1973946986C150194340 @default.
- W1973946986 hasConceptScore W1973946986C158448853 @default.
- W1973946986 hasConceptScore W1973946986C165801399 @default.
- W1973946986 hasConceptScore W1973946986C165864922 @default.
- W1973946986 hasConceptScore W1973946986C179926584 @default.
- W1973946986 hasConceptScore W1973946986C27153228 @default.
- W1973946986 hasConceptScore W1973946986C41895202 @default.
- W1973946986 hasConceptScore W1973946986C48207687 @default.
- W1973946986 hasConceptScore W1973946986C54355233 @default.
- W1973946986 hasConceptScore W1973946986C62520636 @default.
- W1973946986 hasConceptScore W1973946986C70721500 @default.
- W1973946986 hasConceptScore W1973946986C84393581 @default.
- W1973946986 hasConceptScore W1973946986C86339819 @default.
- W1973946986 hasConceptScore W1973946986C86803240 @default.
- W1973946986 hasConceptScore W1973946986C93586867 @default.
- W1973946986 hasConceptScore W1973946986C95444343 @default.
- W1973946986 hasIssue "3" @default.
- W1973946986 hasLocation W19739469861 @default.
- W1973946986 hasOpenAccess W1973946986 @default.
- W1973946986 hasPrimaryLocation W19739469861 @default.
- W1973946986 hasRelatedWork W1967194930 @default.
- W1973946986 hasRelatedWork W1973946986 @default.
- W1973946986 hasRelatedWork W2085510328 @default.
- W1973946986 hasRelatedWork W2086278297 @default.
- W1973946986 hasRelatedWork W2106294913 @default.
- W1973946986 hasRelatedWork W2147022658 @default.
- W1973946986 hasRelatedWork W2156781574 @default.
- W1973946986 hasRelatedWork W2172226517 @default.
- W1973946986 hasRelatedWork W2523193918 @default.
- W1973946986 hasRelatedWork W2610903821 @default.
- W1973946986 hasVolume "96" @default.
- W1973946986 isParatext "false" @default.
- W1973946986 isRetracted "false" @default.
- W1973946986 magId "1973946986" @default.
- W1973946986 workType "article" @default.