Matches in SemOpenAlex for { <https://semopenalex.org/work/W1973998462> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W1973998462 abstract "Free radicals are compounds with an unpaired electron capable of independent existence. These highly reactive species have been implicated in many disease states and can react with cell membranes, lipids, proteins, and DNA. When an oxygen radical reacts with DNA, base damage, cross-linking (e.g., DNA-DNA or DNA-protein), or DNA backbone damage (e.g., single- or double-strand breaks) can occur and often result in cell death. The field of oxidative metabolism as it relates to DNA damage has grown tremendously, with more DNA adducts being identified as biomarkers. These biomarkers are indicative of DNA damage. Measurement of these biomarkers has proved to be a challenge because of their relatively low occurrence (1 per 10(5)-10(6) bases). Methodologies for the measurement of DNA damage include thin-layer chromatography, enzyme-linked immunosorbent assay, gas chromatography-mass spectrometry, DNA sequencing, high-performance liquid chromatography (HPLC)-ultraviolet, and HPLC-ECD. HPLC-ECD (electrochemical detection) is a powerful technique that is both sensitive and selective. However, HPLC-ECD is generally not amenable to gradient analyses, so its utility is restricted. In addition, many of the bases and nucleosides are not electrochemically active. Gradient HPLC separation coupled to both a coulometric electrochemical array detector and an ultraviolet detector overcomes these limitations. Presented here is a gradient HPLC method that measures a wide variety of nucleosides, bases, and hydroxylated adducts using the inherent stability, sensitivity, and wide dynamic range of a coulometric electrochemical array detector and the universal detection qualities of an ultraviolet detector. Linear ranges, limits of detection, and detailed methods development are presented." @default.
- W1973998462 created "2016-06-24" @default.
- W1973998462 creator A5029139687 @default.
- W1973998462 creator A5048735514 @default.
- W1973998462 creator A5056190178 @default.
- W1973998462 date "1999-01-01" @default.
- W1973998462 modified "2023-09-27" @default.
- W1973998462 title "Method for the Detection of Nucleosides, Bases, and Hydroxylated Adducts Using Gradient HPLC with Coulometric Array and Ultraviolet Detection" @default.
- W1973998462 cites W2338707059 @default.
- W1973998462 doi "https://doi.org/10.1089/jmf.1999.2.209" @default.
- W1973998462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19281383" @default.
- W1973998462 hasPublicationYear "1999" @default.
- W1973998462 type Work @default.
- W1973998462 sameAs 1973998462 @default.
- W1973998462 citedByCount "3" @default.
- W1973998462 crossrefType "journal-article" @default.
- W1973998462 hasAuthorship W1973998462A5029139687 @default.
- W1973998462 hasAuthorship W1973998462A5048735514 @default.
- W1973998462 hasAuthorship W1973998462A5056190178 @default.
- W1973998462 hasConcept C108204754 @default.
- W1973998462 hasConcept C109379892 @default.
- W1973998462 hasConcept C143425029 @default.
- W1973998462 hasConcept C147789679 @default.
- W1973998462 hasConcept C17525397 @default.
- W1973998462 hasConcept C178790620 @default.
- W1973998462 hasConcept C179998833 @default.
- W1973998462 hasConcept C185592680 @default.
- W1973998462 hasConcept C43617362 @default.
- W1973998462 hasConcept C52859227 @default.
- W1973998462 hasConcept C552990157 @default.
- W1973998462 hasConcept C55493867 @default.
- W1973998462 hasConceptScore W1973998462C108204754 @default.
- W1973998462 hasConceptScore W1973998462C109379892 @default.
- W1973998462 hasConceptScore W1973998462C143425029 @default.
- W1973998462 hasConceptScore W1973998462C147789679 @default.
- W1973998462 hasConceptScore W1973998462C17525397 @default.
- W1973998462 hasConceptScore W1973998462C178790620 @default.
- W1973998462 hasConceptScore W1973998462C179998833 @default.
- W1973998462 hasConceptScore W1973998462C185592680 @default.
- W1973998462 hasConceptScore W1973998462C43617362 @default.
- W1973998462 hasConceptScore W1973998462C52859227 @default.
- W1973998462 hasConceptScore W1973998462C552990157 @default.
- W1973998462 hasConceptScore W1973998462C55493867 @default.
- W1973998462 hasLocation W19739984621 @default.
- W1973998462 hasLocation W19739984622 @default.
- W1973998462 hasOpenAccess W1973998462 @default.
- W1973998462 hasPrimaryLocation W19739984621 @default.
- W1973998462 hasRelatedWork W1982238842 @default.
- W1973998462 hasRelatedWork W1990300489 @default.
- W1973998462 hasRelatedWork W1999800631 @default.
- W1973998462 hasRelatedWork W2029274121 @default.
- W1973998462 hasRelatedWork W2029965425 @default.
- W1973998462 hasRelatedWork W2043299563 @default.
- W1973998462 hasRelatedWork W2055010973 @default.
- W1973998462 hasRelatedWork W2064834230 @default.
- W1973998462 hasRelatedWork W2073424598 @default.
- W1973998462 hasRelatedWork W2079842517 @default.
- W1973998462 hasRelatedWork W2123485357 @default.
- W1973998462 hasRelatedWork W2168742724 @default.
- W1973998462 hasRelatedWork W2196316295 @default.
- W1973998462 hasRelatedWork W2303029339 @default.
- W1973998462 hasRelatedWork W2325520713 @default.
- W1973998462 hasRelatedWork W2415780215 @default.
- W1973998462 hasRelatedWork W2885447454 @default.
- W1973998462 hasRelatedWork W364955705 @default.
- W1973998462 hasRelatedWork W49475602 @default.
- W1973998462 hasRelatedWork W133164353 @default.
- W1973998462 isParatext "false" @default.
- W1973998462 isRetracted "false" @default.
- W1973998462 magId "1973998462" @default.
- W1973998462 workType "article" @default.