FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974026314> ?p ?o ?g. }

• W1974026314 endingPage "1528" @default.
• W1974026314 startingPage "1522" @default.
• W1974026314 abstract "1. Cyclo-oxygenase (COX) and nitric oxide synthase (NOS) are two enzymes which have distinct cytokine-inducible isoforms (COX-2 and iNOS). Many cytokine receptors have an intracellular tyrosine kinase domain. Here we have used the tyrosine kinase inhibitors, erbstatin and genistein, to investigate the potential role of tyrosine kinase activation in the induction on COX-2 and iNOS caused by endotoxin (lipopolysaccharide; LPS) in bovine aortic endothelial cells (BAEC) and J774.2 macrophages. 2. The main COX metabolites, 6-oxo-prostaglandin F1 alpha (6-oxo-PGF1 alpha) (for BAEC) and PGF2 alpha (for 774.2 macrophages) were measured by radioimmunossay: (i) accumulation of COX metabolites from endogenous arachidonic acid was measured at 24 h after addition of LPS (1 microgram ml-1); (ii) in experiments designed to measure 'COX activity', COX metabolites generated by BAEC or J774.2 macrophages activated with LPS were assayed (at 12 h after LPS administration) after incubation of the washed cells with exogenous arachidonic acid (30 microM for 15 min). Western blot analysis with a specific antibody to COX-2 was used to determine the expression of COX-2 protein caused by LPS in cell extracts. Accumulation of nitrite (measured by the Griess reaction) was used as an indicator of NO formation and, hence, iNOS activity. 3. Erbstatin (0.05 to 5 micrograms ml-1) or genistein (0.5 to 50 micrograms ml-1) caused a dose-dependent inhibition of the accumulation of COX metabolites in the supernatant of BAEC or J774.2 macrophages activated with LPS. Erbstatin or genistein also caused a dose-dependent inhibition of 'COX activity' in both cell types. Western blot analysis showed that erbstatin (5 ig ml1') or genistein (50gg ml-') inhibited the expression of COX-2 protein in BAEC and J774.2 macrophages activated with LPS (lLgml-' for 24 h).4. Erbstatin or genistein also caused a dose-dependent inhibition of nitrite accumulation in J774.2 macrophages activated with LPS (1 sg ml-' for 24 h). In contrast to J774.2 macrophages, BAECstimulated with LPS (1 pg ml-' for 24 h) did not produce detectable amounts (<1PiM) of nitrite.5. These results suggest that tyrosine phosphorylation is part of the signal transduction mechanism that mediates (i) the induction of COX-2 and iNOS elicited by LPS in J774.2 macrophages, and (ii) the induction of COX-2 by LPS in BAEC." @default.
• W1974026314 created "2016-06-24" @default.
• W1974026314 creator A5005920096 @default.
• W1974026314 creator A5027019379 @default.
• W1974026314 creator A5044468329 @default.
• W1974026314 creator A5045479052 @default.
• W1974026314 creator A5059382959 @default.
• W1974026314 date "1994-12-01" @default.
• W1974026314 modified "2023-10-13" @default.
• W1974026314 title "Involvement of tyrosine kinase in the induction of cyclo-oxygenase and nitric oxide synthase by endotoxin in cultured cells" @default.
• W1974026314 cites W1517205234 @default.
• W1974026314 cites W1523695818 @default.
• W1974026314 cites W1539675729 @default.
• W1974026314 cites W1542743692 @default.
• W1974026314 cites W1563962170 @default.
• W1974026314 cites W1586109329 @default.
• W1974026314 cites W1587123510 @default.
• W1974026314 cites W163125452 @default.
• W1974026314 cites W1643013712 @default.
• W1974026314 cites W1656644300 @default.
• W1974026314 cites W1662512830 @default.
• W1974026314 cites W1669371681 @default.
• W1974026314 cites W1675755394 @default.
• W1974026314 cites W1705999641 @default.
• W1974026314 cites W1816763520 @default.
• W1974026314 cites W1870523313 @default.
• W1974026314 cites W1929009802 @default.
• W1974026314 cites W1965077361 @default.
• W1974026314 cites W1968585354 @default.
• W1974026314 cites W1981028890 @default.
• W1974026314 cites W1983759759 @default.
• W1974026314 cites W1988086603 @default.
• W1974026314 cites W1998347276 @default.
• W1974026314 cites W2003392338 @default.
• W1974026314 cites W2003897562 @default.
• W1974026314 cites W2010893307 @default.
• W1974026314 cites W2012810767 @default.
• W1974026314 cites W2015850083 @default.
• W1974026314 cites W2018732312 @default.
• W1974026314 cites W2019843852 @default.
• W1974026314 cites W2021117769 @default.
• W1974026314 cites W2021823569 @default.
• W1974026314 cites W2027152576 @default.
• W1974026314 cites W2029157767 @default.
• W1974026314 cites W2029753827 @default.
• W1974026314 cites W2032359283 @default.
• W1974026314 cites W2032809973 @default.
• W1974026314 cites W2035167648 @default.
• W1974026314 cites W2037768305 @default.
• W1974026314 cites W2042782418 @default.
• W1974026314 cites W2055860776 @default.
• W1974026314 cites W2059379142 @default.
• W1974026314 cites W2060152996 @default.
• W1974026314 cites W2079273569 @default.
• W1974026314 cites W2085680573 @default.
• W1974026314 cites W2089747387 @default.
• W1974026314 cites W2091504157 @default.
• W1974026314 cites W2093547993 @default.
• W1974026314 cites W2094309129 @default.
• W1974026314 cites W2113745276 @default.
• W1974026314 cites W2122048408 @default.
• W1974026314 cites W2137838799 @default.
• W1974026314 cites W2144663935 @default.
• W1974026314 cites W2158686638 @default.
• W1974026314 cites W2169993657 @default.
• W1974026314 cites W2182637261 @default.
• W1974026314 cites W2236419626 @default.
• W1974026314 cites W2254363857 @default.
• W1974026314 cites W2307355608 @default.
• W1974026314 cites W2317057958 @default.
• W1974026314 doi "https://doi.org/10.1111/j.1476-5381.1994.tb17169.x" @default.
• W1974026314 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1510522" @default.
• W1974026314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7534189" @default.
• W1974026314 hasPublicationYear "1994" @default.
• W1974026314 type Work @default.
• W1974026314 sameAs 1974026314 @default.
• W1974026314 citedByCount "91" @default.
• W1974026314 countsByYear W19740263142013 @default.
• W1974026314 countsByYear W19740263142015 @default.
• W1974026314 countsByYear W19740263142018 @default.
• W1974026314 countsByYear W19740263142019 @default.
• W1974026314 countsByYear W19740263142022 @default.
• W1974026314 crossrefType "journal-article" @default.
• W1974026314 hasAuthorship W1974026314A5005920096 @default.
• W1974026314 hasAuthorship W1974026314A5027019379 @default.
• W1974026314 hasAuthorship W1974026314A5044468329 @default.
• W1974026314 hasAuthorship W1974026314A5045479052 @default.
• W1974026314 hasAuthorship W1974026314A5059382959 @default.
• W1974026314 hasBestOaLocation W19740263141 @default.
• W1974026314 hasConcept C134018914 @default.
• W1974026314 hasConcept C153911025 @default.
• W1974026314 hasConcept C170493617 @default.
• W1974026314 hasConcept C181199279 @default.
• W1974026314 hasConcept C185592680 @default.
• W1974026314 hasConcept C2777622882 @default.
• W1974026314 hasConcept C2778078955 @default.
• W1974026314 hasConcept C2778298627 @default.
• W1974026314 hasConcept C2778754761 @default.

• next