Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974047460> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1974047460 endingPage "649" @default.
- W1974047460 startingPage "644" @default.
- W1974047460 abstract "Studies of the multidrug-resistance protein 1 (MRP1) have been hampered by the lack of a simple expression system allowing for rapid generation of mutants and yielding milligram amounts of protein. Here, we describe a Saccharomyces cerevisiae expression system that meets those conditions. MRP1 was expressed under the control of the constitutive PMA1 (yeast proton pump) promoter. The best conditions for expression were determined, including the use of the chemical chaperone glycerol, which increased MRP1 expression. N-terminal poly-histidine or FLAG affinity tags reduce MRP1 expression, whereas the same tags fused to the C-terminus had no effect. All the fusion proteins were functional. We conclude that because of its low cost and simplicity, the S. cerevisiae-based MRP1-expression system will be useful for studies where a large number of mutants or milligram amounts of purified MRP1 are needed." @default.
- W1974047460 created "2016-06-24" @default.
- W1974047460 creator A5017442544 @default.
- W1974047460 creator A5026954687 @default.
- W1974047460 date "2003-07-01" @default.
- W1974047460 modified "2023-09-23" @default.
- W1974047460 title "Expression of functional multidrug-resistance protein 1 in Saccharomyces cerevisiae: effects of N- and C-terminal affinity tags" @default.
- W1974047460 cites W1502426706 @default.
- W1974047460 cites W1517172122 @default.
- W1974047460 cites W1890023926 @default.
- W1974047460 cites W1992490042 @default.
- W1974047460 cites W1992531404 @default.
- W1974047460 cites W2010242646 @default.
- W1974047460 cites W2010735982 @default.
- W1974047460 cites W2011163178 @default.
- W1974047460 cites W2039354424 @default.
- W1974047460 cites W2042141509 @default.
- W1974047460 cites W2048540473 @default.
- W1974047460 cites W2061813158 @default.
- W1974047460 cites W2066412555 @default.
- W1974047460 cites W2076536269 @default.
- W1974047460 cites W2078509303 @default.
- W1974047460 cites W2079356894 @default.
- W1974047460 cites W2159854260 @default.
- W1974047460 cites W2336424366 @default.
- W1974047460 cites W2781444071 @default.
- W1974047460 cites W4252079440 @default.
- W1974047460 doi "https://doi.org/10.1016/s0006-291x(03)01029-5" @default.
- W1974047460 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12810067" @default.
- W1974047460 hasPublicationYear "2003" @default.
- W1974047460 type Work @default.
- W1974047460 sameAs 1974047460 @default.
- W1974047460 citedByCount "20" @default.
- W1974047460 countsByYear W19740474602013 @default.
- W1974047460 countsByYear W19740474602020 @default.
- W1974047460 countsByYear W19740474602022 @default.
- W1974047460 crossrefType "journal-article" @default.
- W1974047460 hasAuthorship W1974047460A5017442544 @default.
- W1974047460 hasAuthorship W1974047460A5026954687 @default.
- W1974047460 hasConcept C104317684 @default.
- W1974047460 hasConcept C123894998 @default.
- W1974047460 hasConcept C133936738 @default.
- W1974047460 hasConcept C143065580 @default.
- W1974047460 hasConcept C156904656 @default.
- W1974047460 hasConcept C22744801 @default.
- W1974047460 hasConcept C2777576037 @default.
- W1974047460 hasConcept C2778460671 @default.
- W1974047460 hasConcept C2779222958 @default.
- W1974047460 hasConcept C40767141 @default.
- W1974047460 hasConcept C501593827 @default.
- W1974047460 hasConcept C515207424 @default.
- W1974047460 hasConcept C55493867 @default.
- W1974047460 hasConcept C86803240 @default.
- W1974047460 hasConcept C95444343 @default.
- W1974047460 hasConceptScore W1974047460C104317684 @default.
- W1974047460 hasConceptScore W1974047460C123894998 @default.
- W1974047460 hasConceptScore W1974047460C133936738 @default.
- W1974047460 hasConceptScore W1974047460C143065580 @default.
- W1974047460 hasConceptScore W1974047460C156904656 @default.
- W1974047460 hasConceptScore W1974047460C22744801 @default.
- W1974047460 hasConceptScore W1974047460C2777576037 @default.
- W1974047460 hasConceptScore W1974047460C2778460671 @default.
- W1974047460 hasConceptScore W1974047460C2779222958 @default.
- W1974047460 hasConceptScore W1974047460C40767141 @default.
- W1974047460 hasConceptScore W1974047460C501593827 @default.
- W1974047460 hasConceptScore W1974047460C515207424 @default.
- W1974047460 hasConceptScore W1974047460C55493867 @default.
- W1974047460 hasConceptScore W1974047460C86803240 @default.
- W1974047460 hasConceptScore W1974047460C95444343 @default.
- W1974047460 hasIssue "3" @default.
- W1974047460 hasLocation W19740474601 @default.
- W1974047460 hasLocation W19740474602 @default.
- W1974047460 hasOpenAccess W1974047460 @default.
- W1974047460 hasPrimaryLocation W19740474601 @default.
- W1974047460 hasRelatedWork W1776753901 @default.
- W1974047460 hasRelatedWork W1965684540 @default.
- W1974047460 hasRelatedWork W1975929799 @default.
- W1974047460 hasRelatedWork W2062385823 @default.
- W1974047460 hasRelatedWork W2072731694 @default.
- W1974047460 hasRelatedWork W2087346710 @default.
- W1974047460 hasRelatedWork W2094041192 @default.
- W1974047460 hasRelatedWork W2096718270 @default.
- W1974047460 hasRelatedWork W2102055466 @default.
- W1974047460 hasRelatedWork W3120238434 @default.
- W1974047460 hasVolume "306" @default.
- W1974047460 isParatext "false" @default.
- W1974047460 isRetracted "false" @default.
- W1974047460 magId "1974047460" @default.
- W1974047460 workType "article" @default.