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- W1974077194 abstract "A new bifunctional affinity label, 5′-p-(fluorosulfonyl)benzoyl-8-azidoadenosine (5′FSBAzA), has been synthesized by condensation of p-(fluorosulfonyl)benzoyl chloride with 8-azidoadenosine. 5′-FSBAzA has been characterized by elemental analysis, thinlayer chromatography, and ultraviolet and 1H NMR spectroscopy. The affinity label contains both an electrophilic fluorosulfonyl moiety and a photoactivatable azido group which are capable of reacting with several classes of amino acids found in enzymes. 5′FSBAzA reacts with bovine liver glutamate dehydrogenase in a two-step process: a dark reaction yielding about 0.5 mol of the sulfonylbenzoyl-8-azidoadenosine (SBAzA) group bound/mol enzyme subunit by reaction of the enzyme at the fluorosulfonyl group, followed by photolysis in which 25% of the covalently bound SBAzA becomes crosslinked to the enzyme. 5′-FSBAzA-modified glutamate dehydrogenase, both before and after photolysis, retains full catalytic activity but is less sensitive to allosteric inhibition by GTP, to activation by ADP, and to inhibition by 1 mm NADH. These results suggest the modification in the dark reaction of a regulatory nucleotide binding site. Photoactivation of the covalently bound reagent may have general applicability in relating modified amino acids which are close to each other in the region of the purine nucleotide binding sites of glutamate dehydrogenase and other proteins." @default.
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- W1974077194 date "1989-11-01" @default.
- W1974077194 modified "2023-09-27" @default.
- W1974077194 title "5′-p-(Fluorosulfonyl)benzoyl-8-azidoadenosine: A new bifunctional affinity label for nucleotide binding sites in proteins" @default.
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- W1974077194 doi "https://doi.org/10.1016/0003-9861(89)90377-9" @default.
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