Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974085059> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W1974085059 endingPage "234" @default.
- W1974085059 startingPage "227" @default.
- W1974085059 abstract "Abstract The retention behavior of a series of negatively charged β-galactosidase fusion proteins carrying an additional 1 (designated BGCD1), 5 (BGCD5), 11 (BGCD11), and 16 (BGCD16) aspartate residues was studied. The added tails promoted protein retention in order of tail length and were most effective closer to the isoelectric point. The two parameters Z and I , obtained from the stoichiometric displacement model, were used to characterize the extent of binding between the protein and the ion-exchange surface. At pH 5.7, the Z number increased with tail length (charge) and was 11.5, 8.5, 6.9 and 5.3 for BGCD11, BGCD5, BGCD1 and wild type β-galactosidase (BGWT), respectively. At these conditions, the fusions had very similar I values which were five times smaller than that of BGWT. However, the increase in Z numbers outweighed the decrease in I values and retention was enhanced. When BGWT was brought to the same net charge (by increasing the mobile phase pH) as each of the fusions, the Z number was similar to that of the corresponding fusion. However, the I value decreased with increasing pH (net charge) and was lower than that of the corresponding fusion by factors of 25–500. Consequently, despite the similar Z numbers, the fusions still had higher retention." @default.
- W1974085059 created "2016-06-24" @default.
- W1974085059 creator A5020590353 @default.
- W1974085059 creator A5039991156 @default.
- W1974085059 date "1995-01-01" @default.
- W1974085059 modified "2023-10-14" @default.
- W1974085059 title "Charged fusions for β-galactosidase retention in anion-exchange chromatography" @default.
- W1974085059 cites W1966625641 @default.
- W1974085059 cites W1972620015 @default.
- W1974085059 cites W1980149555 @default.
- W1974085059 cites W1981925074 @default.
- W1974085059 cites W2000541359 @default.
- W1974085059 cites W2018444542 @default.
- W1974085059 cites W2019964301 @default.
- W1974085059 cites W2020533692 @default.
- W1974085059 cites W2025564651 @default.
- W1974085059 cites W2035758756 @default.
- W1974085059 cites W2037396947 @default.
- W1974085059 cites W2063820532 @default.
- W1974085059 cites W2072745156 @default.
- W1974085059 cites W2074046988 @default.
- W1974085059 cites W2075615946 @default.
- W1974085059 cites W2078269273 @default.
- W1974085059 cites W2120761767 @default.
- W1974085059 cites W2234332767 @default.
- W1974085059 doi "https://doi.org/10.1016/0021-9673(94)00970-k" @default.
- W1974085059 hasPublicationYear "1995" @default.
- W1974085059 type Work @default.
- W1974085059 sameAs 1974085059 @default.
- W1974085059 citedByCount "4" @default.
- W1974085059 countsByYear W19740850592018 @default.
- W1974085059 crossrefType "journal-article" @default.
- W1974085059 hasAuthorship W1974085059A5020590353 @default.
- W1974085059 hasAuthorship W1974085059A5039991156 @default.
- W1974085059 hasConcept C116817701 @default.
- W1974085059 hasConcept C117760992 @default.
- W1974085059 hasConcept C145148216 @default.
- W1974085059 hasConcept C178790620 @default.
- W1974085059 hasConcept C179998833 @default.
- W1974085059 hasConcept C185592680 @default.
- W1974085059 hasConcept C206967267 @default.
- W1974085059 hasConcept C43617362 @default.
- W1974085059 hasConceptScore W1974085059C116817701 @default.
- W1974085059 hasConceptScore W1974085059C117760992 @default.
- W1974085059 hasConceptScore W1974085059C145148216 @default.
- W1974085059 hasConceptScore W1974085059C178790620 @default.
- W1974085059 hasConceptScore W1974085059C179998833 @default.
- W1974085059 hasConceptScore W1974085059C185592680 @default.
- W1974085059 hasConceptScore W1974085059C206967267 @default.
- W1974085059 hasConceptScore W1974085059C43617362 @default.
- W1974085059 hasIssue "2" @default.
- W1974085059 hasLocation W19740850591 @default.
- W1974085059 hasOpenAccess W1974085059 @default.
- W1974085059 hasPrimaryLocation W19740850591 @default.
- W1974085059 hasRelatedWork W1964747279 @default.
- W1974085059 hasRelatedWork W1989436565 @default.
- W1974085059 hasRelatedWork W2024912340 @default.
- W1974085059 hasRelatedWork W2056490804 @default.
- W1974085059 hasRelatedWork W2067546308 @default.
- W1974085059 hasRelatedWork W2072817592 @default.
- W1974085059 hasRelatedWork W2142640154 @default.
- W1974085059 hasRelatedWork W2382739960 @default.
- W1974085059 hasRelatedWork W2405995553 @default.
- W1974085059 hasRelatedWork W827985110 @default.
- W1974085059 hasVolume "689" @default.
- W1974085059 isParatext "false" @default.
- W1974085059 isRetracted "false" @default.
- W1974085059 magId "1974085059" @default.
- W1974085059 workType "article" @default.