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• W1974098241 abstract "We evaluated the photochemical and enzymatic synthesis of methanol from formaldehyde with alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae and NAD+ photoreduction by the visible-light sensitization of zinc tetraphenylporphyrin tetrasulfonate (ZnTPPS) in the presence of methylviologen (MV2+), diaphorase, and triethanolamine (TEOA). When the sample solution containing ZnTPPS, MV2+, NAD+, diaphorase, and TEOA in potassium phosphate buffer solution was irradiated, the NADH produced increased with the irradiation time. After irradiation for 180 min, the conversion yield of NAD+ to NADH was about 60% under 0.1 mM NAD+ condition. The methanol production also depended on the conversion yield of NAD+ to NADH. After irradiation for 180 min, 0.38 μM of methanol was produced from formaldehyde (16 μM). The conversion ratio of formaldehyde to methanol was about 2.3%. This result indicates that a system for the photochemical synthesis of methanol from formaldehyde was developed with ADH and the NADH produced by the photosensitization of ZnTPPS in water media." @default.
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• W1974098241 date "2007-01-01" @default.
• W1974098241 modified "2023-10-14" @default.
• W1974098241 title "Photochemical and enzymatic synthesis of methanol from formaldehyde with alcohol dehydrogenase from Saccharomyces cerevisiae and water-soluble zinc porphyrin" @default.
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• W1974098241 doi "https://doi.org/10.1016/j.molcatb.2006.08.001" @default.
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