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- W1974181307 abstract "In mutation breeding programs for many cereal crops, pollen irradiation has not proved to be a practical method for mutation induction, although theoretically the method of choice. The more commonly used procedure, that of irradiating dry seeds containing fully differentiated, “mature” embryos, has as its principal drawback the small size of the isomutant-carrying sector which can be produced in the R-1 plant. As a consequence of the small R-2 isomutant populations thus available for testing, screening procedures must usually be delayed until the R-3 or later generations. In this study, irradiation of proembryo stages has been investigated with regard to the practicality of using this method for mutation induction in cereal crops such as barley. By using albino mutations as genetic sector markers, we find that irradiation of barley proembryos with a relatively low total dose will yield much larger isomutant-carrying sectors and consequently far greater number of isomutant R-2 plants, with a comparable mutation frequency and less sterility than can be obtained by dry seed irradiation. Of great importance to the plant breeder is the possibility that the screening for less drastic mutations and multiple testing can be carried out at least one generation earlier by virtue of the larger isomutant populations produced by proembryo irradiation. Despite the more cumbersome handling involved during radiation treatment, the advantages which proembryo irradiation affords over dry seed irradiation would seem to recommend this method for cereal crops in those situations wherein pollen irradiation is not practical." @default.
- W1974181307 created "2016-06-24" @default.
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- W1974181307 date "1961-01-01" @default.
- W1974181307 modified "2023-09-23" @default.
- W1974181307 title "Mutation induction by proembryo irradiation" @default.
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- W1974181307 doi "https://doi.org/10.1016/s0033-7560(61)80026-4" @default.
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