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- W1974203103 abstract "Lysogenic cells of Escherichia coli K12 (λ+) and Escherichia coli M3 (λ59) were held in atmospheres having relative humidities (R.H.) from 30% to 80% and irradiated with 2537 Å ultraviolet light (uv.). The colony-forming ability of both types of cell was destroyed more rapidly at 55% R.H. than at any other level of R.H. With E. coli K12 (λ+) the percentage of cells in which prophage induction occurred increased as the dose of ultraviolet light increased and the maximum number of inductions occurred at 55% R.H. Inositol prevented (λ+) prophage induction but was less effective in doing so at 55% R.H. Desiccation alone induced the (λ59) prophage and subsequent uv. irradiation resulted in the destruction of the prophage. The maximum rate of destruction was found at 55% R.H. Inositol prevented the uv. inactivation of the (λ59) prophage, resulting in an increase in inductions with uv. dose at 30% R.H. The free phages were found to have the same sensitivity to ultraviolet light as the induced prophages but were less protected by inositol.It is proposed that water molecules hold the prophage to or in the host DNA and that ultraviolet light induces the prophage and destroys its integrity by reorientating these water molecules." @default.
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- W1974203103 date "1967-03-01" @default.
- W1974203103 modified "2023-09-24" @default.
- W1974203103 title "BOUND WATER, INOSITOL, AND THE INDUCTION OF LAMBDA PROPHAGES BY ULTRAVIOLET LIGHT" @default.
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- W1974203103 doi "https://doi.org/10.1139/m67-039" @default.
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