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- W1974225997 abstract "Homing endonucleases are highly specific DNA-cleaving enzymes that recognize long stretches of DNA. The engineering of these enzymes provides novel instruments for genome modification in a wide range of fields, including gene targeting, by inducing specific double-strand breaks. I-CvuI is a homing endonuclease from the green alga Chlorella vulgaris . This enzyme was purified after overexpression in Escherichia coli . Crystallization experiments of I-CvuI in complex with its DNA target in the presence of Mg 2+ yielded crystals suitable for X-ray diffraction analysis. The crystals belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit-cell parameters a = 62.83, b = 83.56, c = 94.40 Å. The self-rotation function and the Matthews coefficient suggested the presence of one protein–DNA complex per asymmetric unit. The crystals diffracted to a resolution limit of 1.9 Å using synchrotron radiation." @default.
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- W1974225997 date "2014-01-22" @default.
- W1974225997 modified "2023-10-16" @default.
- W1974225997 title "Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from<i>Chlorella vulgaris</i>in complex with its target DNA" @default.
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- W1974225997 doi "https://doi.org/10.1107/s2053230x1400065x" @default.
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