Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974371989> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1974371989 endingPage "1405" @default.
- W1974371989 startingPage "1401" @default.
- W1974371989 abstract "Human insulin-like growth factor 1 (hIGF-1) is one kind of growth factor with clinical significance in medicine. The expression of TrxA-hIGF-1 fusion protein was rationally compared in three different Escherichia coli hosts (BL21 (DE3), Rosetta (DE3) and Rosetta-gami (DE3)) with the transformation of plasmid pET32-hIGF-1. The highest productivity of soluble hIGF-1 fusion protein was achieved in E. coli Rosetta-gami (DE3). Moreover, the effects of different expression conditions in this E. coli Rosetta-gami (DE3)/pET32-hIGF-1 host were systematically investigated to improve the expression level of the fusion protein. Under the optimized conditions, a high percent of the target fusion protein (96%) was expressed as soluble form with the volumetric production of soluble fusion protein reaching up to 2.06 g/L. After cell disruption, the soluble fusion protein was separated effectively by affinity chromatography and cleaved by enterokinase, with the concentration of mature hIGF-1 reaching up to 0.42 g/L in the mixture. The present work should be useful for the enhanced production of soluble protein with multiple disulfide bonds in E. coli." @default.
- W1974371989 created "2016-06-24" @default.
- W1974371989 creator A5011617046 @default.
- W1974371989 creator A5019278466 @default.
- W1974371989 creator A5034113666 @default.
- W1974371989 creator A5049411779 @default.
- W1974371989 creator A5052493543 @default.
- W1974371989 creator A5060185955 @default.
- W1974371989 creator A5061254054 @default.
- W1974371989 date "2010-08-01" @default.
- W1974371989 modified "2023-10-02" @default.
- W1974371989 title "High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli" @default.
- W1974371989 cites W1795716959 @default.
- W1974371989 cites W1931534490 @default.
- W1974371989 cites W1979456422 @default.
- W1974371989 cites W1981470006 @default.
- W1974371989 cites W1991516961 @default.
- W1974371989 cites W1998815656 @default.
- W1974371989 cites W2004422662 @default.
- W1974371989 cites W2020497113 @default.
- W1974371989 cites W2029580699 @default.
- W1974371989 cites W2034611626 @default.
- W1974371989 cites W2041183688 @default.
- W1974371989 cites W2048224470 @default.
- W1974371989 cites W2060043772 @default.
- W1974371989 cites W2060094399 @default.
- W1974371989 cites W2075657732 @default.
- W1974371989 cites W2090645380 @default.
- W1974371989 cites W2119888673 @default.
- W1974371989 cites W2134857592 @default.
- W1974371989 cites W2150699248 @default.
- W1974371989 cites W2163866049 @default.
- W1974371989 cites W220296281 @default.
- W1974371989 cites W2782303467 @default.
- W1974371989 doi "https://doi.org/10.1016/j.procbio.2010.05.014" @default.
- W1974371989 hasPublicationYear "2010" @default.
- W1974371989 type Work @default.
- W1974371989 sameAs 1974371989 @default.
- W1974371989 citedByCount "13" @default.
- W1974371989 countsByYear W19743719892013 @default.
- W1974371989 countsByYear W19743719892015 @default.
- W1974371989 countsByYear W19743719892016 @default.
- W1974371989 countsByYear W19743719892017 @default.
- W1974371989 countsByYear W19743719892019 @default.
- W1974371989 countsByYear W19743719892020 @default.
- W1974371989 countsByYear W19743719892022 @default.
- W1974371989 countsByYear W19743719892023 @default.
- W1974371989 crossrefType "journal-article" @default.
- W1974371989 hasAuthorship W1974371989A5011617046 @default.
- W1974371989 hasAuthorship W1974371989A5019278466 @default.
- W1974371989 hasAuthorship W1974371989A5034113666 @default.
- W1974371989 hasAuthorship W1974371989A5049411779 @default.
- W1974371989 hasAuthorship W1974371989A5052493543 @default.
- W1974371989 hasAuthorship W1974371989A5060185955 @default.
- W1974371989 hasAuthorship W1974371989A5061254054 @default.
- W1974371989 hasConcept C104317684 @default.
- W1974371989 hasConcept C123894998 @default.
- W1974371989 hasConcept C153911025 @default.
- W1974371989 hasConcept C185592680 @default.
- W1974371989 hasConcept C2776985332 @default.
- W1974371989 hasConcept C40767141 @default.
- W1974371989 hasConcept C547475151 @default.
- W1974371989 hasConcept C55493867 @default.
- W1974371989 hasConcept C6856738 @default.
- W1974371989 hasConcept C86803240 @default.
- W1974371989 hasConcept C89423630 @default.
- W1974371989 hasConceptScore W1974371989C104317684 @default.
- W1974371989 hasConceptScore W1974371989C123894998 @default.
- W1974371989 hasConceptScore W1974371989C153911025 @default.
- W1974371989 hasConceptScore W1974371989C185592680 @default.
- W1974371989 hasConceptScore W1974371989C2776985332 @default.
- W1974371989 hasConceptScore W1974371989C40767141 @default.
- W1974371989 hasConceptScore W1974371989C547475151 @default.
- W1974371989 hasConceptScore W1974371989C55493867 @default.
- W1974371989 hasConceptScore W1974371989C6856738 @default.
- W1974371989 hasConceptScore W1974371989C86803240 @default.
- W1974371989 hasConceptScore W1974371989C89423630 @default.
- W1974371989 hasIssue "8" @default.
- W1974371989 hasLocation W19743719891 @default.
- W1974371989 hasOpenAccess W1974371989 @default.
- W1974371989 hasPrimaryLocation W19743719891 @default.
- W1974371989 hasRelatedWork W1974371989 @default.
- W1974371989 hasRelatedWork W1974608326 @default.
- W1974371989 hasRelatedWork W2238024296 @default.
- W1974371989 hasRelatedWork W2348991363 @default.
- W1974371989 hasRelatedWork W2356557998 @default.
- W1974371989 hasRelatedWork W2361670818 @default.
- W1974371989 hasRelatedWork W2365212916 @default.
- W1974371989 hasRelatedWork W2381845779 @default.
- W1974371989 hasRelatedWork W2389219739 @default.
- W1974371989 hasRelatedWork W3149679323 @default.
- W1974371989 hasVolume "45" @default.
- W1974371989 isParatext "false" @default.
- W1974371989 isRetracted "false" @default.
- W1974371989 magId "1974371989" @default.
- W1974371989 workType "article" @default.