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- W1974382014 abstract "The thermodynamics of nine hairpin DNAs were evaluated using UV-monitored melting curves and differential scanning calorimetry (DSC). Each DNA has the same five-base loop and a stem with 8−10 base pairs. Five of the DNAs have a tandem mismatch in the stem, while four have all base pairs. The tandem mismatches examined (ga/ga, aa/gc, ca/gc, ta/ac, and tc/tc) spanned the range of stability observed for this motif in a previous study of 28 tandem mismatches. UV-monitored melting curves were obtained in 1.0 M Na+, 0.1 M Na+, and 0.1 M Na+ with 5 mM Mg2+. DSC studies were conducted in 0.1 M Na+. Transition Tm values were unchanged over a 50-fold range of strand concentration. Model-independent enthalpy changes (ΔH°) evaluated by DSC were in good agreement (±8%) with enthalpy values determined by van't Hoff analyses of the melting curves in 0.1 M Na+. The average heat capacity change (ΔCp) associated with the hairpin to single strands transitions was estimated from plots of ΔH° and ΔS° with Tm and ln Tm, respectively, and from profiles of DSC curves. The average ΔCp values (113 ± 9 and 42 ± 27 cal·K-1·mol-1 of bp), were in the range of values reported in previous studies. Consideration of ΔCp produced large changes in ΔH° and ΔS° extrapolated from the transition region to 37 °C and smaller but significant changes to free energies. The loop free energy of the five tandem mismatches at 37 °C varied over a range of ∼4 kcal·mol-1 for each solvent." @default.
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- W1974382014 date "2004-07-10" @default.
- W1974382014 modified "2023-10-01" @default.
- W1974382014 title "Thermodynamic Stability of DNA Tandem Mismatches" @default.
- W1974382014 cites W1529084957 @default.
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- W1974382014 doi "https://doi.org/10.1021/bi049161w" @default.
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