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- W1974391265 abstract "Abstract The 2-monoacylglycerol acyltransferase (EC 2.3.1.22, acylglycerol palmitoyl transferase) catalyzes the synthesis of X -1,2-diacylglycerols from 2-monoacylglycerol and acyl CoA with an apparently variable stereochemical specificity. A microassay for determining the ratio of sn-1,2- and sn-2,3-diacylglycerols formed by the acylation of radioactive 2-monoacylglycerol in intact cells or in cell-free systems in the presence of free fatty acids and cofactors has been developed. The diacylglycerols are isolated by thin-layer chromatography using nonradioactive racemic diacylglycerols as carriers. The enantiomer content is determined following a chemical synthesis of X -1,2-diacylphosphatidylcholines and a stereospecific stepwise release of the sn-1,2- and sn-2,3-diacylglycerols by phospholipase C. By using thin-layer chromatography for the isolation of the hydrolysis products, known samples ranging in enantiomer ratios from 0.05 to 20 and containing 5000 to 200,000 cpm can be assayed to within 1% of the major and within 10% of the minor enatiomer content. The method is applicable to the determination of the enantiomer content of X -1,2-diacylglycerols generated via other acyltransferases and via lipolysis of triacylglycerols and diacylglycerolphospholipids in other biological systems." @default.
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- W1974391265 date "1982-01-01" @default.
- W1974391265 modified "2023-09-27" @default.
- W1974391265 title "Radioassay of the stereospecificity of 2-monoacylglycerol acyltransferase" @default.
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- W1974391265 doi "https://doi.org/10.1016/0003-2697(82)90657-1" @default.
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