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- W1974460422 abstract "The biosynthesis and turnover of nicotinamide adenine dinucleotide (NAD) have been examined in mitotic cells of the human culture line, D98/AH2. No significant difference in the incorporation of nicotinic acid or nicotinamide could be detected between mitotic and interphase cells. The distribution of newly-incorporated nicotinic acid among the various pyridine nucleotides was also identical in mitotic and interphase cells. Whereas previous results have shown that the nucleus is necessary for NAD biosynthesis, the present results show that an intact nucleus is not required. In contrast to the equivalent rates of biosynthesis in mitotic and interphase cells, the pyridine ring of NAD was lost twice as fast from mitotic as from interphase cells. Loss of the pyridine ring to the medium is not necessarily an accurate measure of turnover, and the difference between mitotic and interphase cells may reflect differential reutilization of the pyridine ring within the cell. However, it is clear that NAD turnover is substantial in mitotic cells and possibly greater in mitotic cells than interphase cells." @default.
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- W1974460422 date "1975-12-01" @default.
- W1974460422 modified "2023-09-27" @default.
- W1974460422 title "Pyridine nucleotide metabolism in mitotic cells" @default.
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- W1974460422 doi "https://doi.org/10.1002/jcp.1040860509" @default.
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