Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974525344> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1974525344 endingPage "8831" @default.
- W1974525344 startingPage "8825" @default.
- W1974525344 abstract "Carbamoyl phosphate synthetase from Escherichia coli catalyzes the production of carbamoyl phosphate from two molecules of Mg2+ATP, one molecule of bicarbonate, and one molecule of glutamine. The enzyme consists of two polypeptide chains referred to as the large and small subunits. While the large subunit provides the active sites responsible for the binding of nucleotides and other effector ligands, the small subunit contains those amino acid residues that catalyze the hydrolysis of glutamine to glutamate and ammonia. From both amino acid sequence analyses and structural studies it is now known that the small subunit belongs to the class I amidotransferase family of enzymes. Numerous biochemical studies have suggested that the reaction mechanism of the small subunit proceeds through the formation of the glutamyl thioester intermediate and that both Cys 269 and His 353 are critical for catalysis. Here we describe the X-ray crystallographic structure of carbamoyl phosphate synthetase from E. coli in which His 353 has been replaced with an asparagine residue. Crystals employed in the investigation were grown in the presence of glutamine, and the model has been refined to a crystallographic R-factor of 19.1% for all measured X-ray data from 30 to 1.8 Å resolution. The active site of the small subunit clearly contains a covalently bound thioester intermediate at Cys 269, and indeed, this investigation provides the first direct structural observation of an enzyme intermediate in the amidotransferase family." @default.
- W1974525344 created "2016-06-24" @default.
- W1974525344 creator A5002277619 @default.
- W1974525344 creator A5008538571 @default.
- W1974525344 creator A5011814024 @default.
- W1974525344 creator A5031420273 @default.
- W1974525344 creator A5049401708 @default.
- W1974525344 creator A5086916844 @default.
- W1974525344 date "1998-06-01" @default.
- W1974525344 modified "2023-09-23" @default.
- W1974525344 title "Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis<sup>,</sup>" @default.
- W1974525344 cites W1518848487 @default.
- W1974525344 cites W1582640966 @default.
- W1974525344 cites W1963846992 @default.
- W1974525344 cites W2057984729 @default.
- W1974525344 cites W2060534633 @default.
- W1974525344 cites W2080476827 @default.
- W1974525344 cites W2086011717 @default.
- W1974525344 doi "https://doi.org/10.1021/bi9807761" @default.
- W1974525344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9636022" @default.
- W1974525344 hasPublicationYear "1998" @default.
- W1974525344 type Work @default.
- W1974525344 sameAs 1974525344 @default.
- W1974525344 citedByCount "79" @default.
- W1974525344 countsByYear W19745253442012 @default.
- W1974525344 countsByYear W19745253442013 @default.
- W1974525344 countsByYear W19745253442014 @default.
- W1974525344 countsByYear W19745253442015 @default.
- W1974525344 countsByYear W19745253442016 @default.
- W1974525344 countsByYear W19745253442018 @default.
- W1974525344 countsByYear W19745253442019 @default.
- W1974525344 countsByYear W19745253442020 @default.
- W1974525344 countsByYear W19745253442021 @default.
- W1974525344 countsByYear W19745253442022 @default.
- W1974525344 crossrefType "journal-article" @default.
- W1974525344 hasAuthorship W1974525344A5002277619 @default.
- W1974525344 hasAuthorship W1974525344A5008538571 @default.
- W1974525344 hasAuthorship W1974525344A5011814024 @default.
- W1974525344 hasAuthorship W1974525344A5031420273 @default.
- W1974525344 hasAuthorship W1974525344A5049401708 @default.
- W1974525344 hasAuthorship W1974525344A5086916844 @default.
- W1974525344 hasConcept C104292427 @default.
- W1974525344 hasConcept C104317684 @default.
- W1974525344 hasConcept C181199279 @default.
- W1974525344 hasConcept C185592680 @default.
- W1974525344 hasConcept C2775895851 @default.
- W1974525344 hasConcept C2778834346 @default.
- W1974525344 hasConcept C2779289688 @default.
- W1974525344 hasConcept C2779349466 @default.
- W1974525344 hasConcept C2908748535 @default.
- W1974525344 hasConcept C41183919 @default.
- W1974525344 hasConcept C512185932 @default.
- W1974525344 hasConcept C515207424 @default.
- W1974525344 hasConcept C55493867 @default.
- W1974525344 hasConcept C71240020 @default.
- W1974525344 hasConcept C74539022 @default.
- W1974525344 hasConcept C75972863 @default.
- W1974525344 hasConceptScore W1974525344C104292427 @default.
- W1974525344 hasConceptScore W1974525344C104317684 @default.
- W1974525344 hasConceptScore W1974525344C181199279 @default.
- W1974525344 hasConceptScore W1974525344C185592680 @default.
- W1974525344 hasConceptScore W1974525344C2775895851 @default.
- W1974525344 hasConceptScore W1974525344C2778834346 @default.
- W1974525344 hasConceptScore W1974525344C2779289688 @default.
- W1974525344 hasConceptScore W1974525344C2779349466 @default.
- W1974525344 hasConceptScore W1974525344C2908748535 @default.
- W1974525344 hasConceptScore W1974525344C41183919 @default.
- W1974525344 hasConceptScore W1974525344C512185932 @default.
- W1974525344 hasConceptScore W1974525344C515207424 @default.
- W1974525344 hasConceptScore W1974525344C55493867 @default.
- W1974525344 hasConceptScore W1974525344C71240020 @default.
- W1974525344 hasConceptScore W1974525344C74539022 @default.
- W1974525344 hasConceptScore W1974525344C75972863 @default.
- W1974525344 hasIssue "25" @default.
- W1974525344 hasLocation W19745253441 @default.
- W1974525344 hasLocation W19745253442 @default.
- W1974525344 hasOpenAccess W1974525344 @default.
- W1974525344 hasPrimaryLocation W19745253441 @default.
- W1974525344 hasRelatedWork W1506041082 @default.
- W1974525344 hasRelatedWork W1725499016 @default.
- W1974525344 hasRelatedWork W1874448345 @default.
- W1974525344 hasRelatedWork W1965277029 @default.
- W1974525344 hasRelatedWork W1974525344 @default.
- W1974525344 hasRelatedWork W1992120544 @default.
- W1974525344 hasRelatedWork W204527770 @default.
- W1974525344 hasRelatedWork W2065172938 @default.
- W1974525344 hasRelatedWork W2150377117 @default.
- W1974525344 hasRelatedWork W2171929617 @default.
- W1974525344 hasVolume "37" @default.
- W1974525344 isParatext "false" @default.
- W1974525344 isRetracted "false" @default.
- W1974525344 magId "1974525344" @default.
- W1974525344 workType "article" @default.