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- W1974590646 abstract "Infrared multiphoton dissociation (IRMPD) in a quadrupole ion trap coupled to high-performance liquid chromatography allows the selective dissociation of phosphorylated peptides in mixtures following chromatographic separation. This method is shown to be effective for differentiation of phosphorylated peptides from unphosphorylated ones; only the abundances of the phosphorylated species are appreciably decreased following exposure to 125 ms of 10.6-microm radiation. This LC-IRMPD-MS strategy is demonstrated for a mock mixture of peptides and a tryptic digest of alphaS1-casein. The ability of this technique to differentiate peptides based on phosphorylation state is unaffected by whether the peptides are protonated or sodium-cationized." @default.
- W1974590646 created "2016-06-24" @default.
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- W1974590646 date "2005-08-02" @default.
- W1974590646 modified "2023-09-30" @default.
- W1974590646 title "Differentiation of Phosphorylated and Unphosphorylated Peptides by High-Performance Liquid Chromatography-Electrospray Ionization-Infrared Multiphoton Dissociation in a Quadrupole Ion Trap" @default.
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- W1974590646 doi "https://doi.org/10.1021/ac0509410" @default.
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