FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974634319> ?p ?o ?g. }

• W1974634319 endingPage "3845" @default.
• W1974634319 startingPage "3836" @default.
• W1974634319 abstract "Even though mRNA quantification provides significant information for biological analysis, current methods such as Northern blot analysis and real-time PCR are known to be laborious and lacking in precision. In this study, we demonstrate a new precise mRNA quantification method using CE based on SSCP (CE-SSCP) coupled with reverse transcription. mRNA samples could be simply analyzed for the quantification directly with reverse transcript obtained from a single reaction. This helps to avoid considerable errors generated by a series of the tedious manual steps. Also, unlike real-time PCR, reverse transcripts can be directly quantified by CE-SSCP in this method without further data estimation. Reproducibility and accuracy of CE-SSCP for mRNA quantification was examined using enhanced green fluorescent protein (eGFP) mRNA transcribed in vitro. Specific reverse transcription primer was determined for the accurate quantification of eGFP mRNA from total RNA obtained from the recombinant Escherichia coli. Using elongation factor Tu mRNA as an internal standard, it was shown that sample-to-sample variation could be minimized. Expression kinetics at both mRNA level and protein level was studied and the potential of CE-SSCP in expression analysis was demonstrated by comparison with the eGFP activity assay." @default.
• W1974634319 created "2016-06-24" @default.
• W1974634319 creator A5040514412 @default.
• W1974634319 creator A5057869475 @default.
• W1974634319 creator A5061478337 @default.
• W1974634319 creator A5074852194 @default.
• W1974634319 creator A5079269717 @default.
• W1974634319 creator A5035334875 @default.
• W1974634319 date "2006-10-01" @default.
• W1974634319 modified "2023-10-09" @default.
• W1974634319 title "A precise mRNA quantification method using CE-based SSCP" @default.
• W1974634319 cites W1557474973 @default.
• W1974634319 cites W1569955072 @default.
• W1974634319 cites W157208099 @default.
• W1974634319 cites W2012629229 @default.
• W1974634319 cites W2014716195 @default.
• W1974634319 cites W2015289624 @default.
• W1974634319 cites W2025575091 @default.
• W1974634319 cites W2027483198 @default.
• W1974634319 cites W2041485397 @default.
• W1974634319 cites W2041523376 @default.
• W1974634319 cites W2070070088 @default.
• W1974634319 cites W2077458979 @default.
• W1974634319 cites W2080734541 @default.
• W1974634319 cites W2088468751 @default.
• W1974634319 cites W2091665357 @default.
• W1974634319 cites W2094883982 @default.
• W1974634319 cites W2104947102 @default.
• W1974634319 cites W2122749842 @default.
• W1974634319 cites W2143714980 @default.
• W1974634319 cites W2145684092 @default.
• W1974634319 cites W2152629282 @default.
• W1974634319 cites W2159656294 @default.
• W1974634319 cites W2164578725 @default.
• W1974634319 cites W4236086867 @default.
• W1974634319 doi "https://doi.org/10.1002/elps.200600225" @default.
• W1974634319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16960841" @default.
• W1974634319 hasPublicationYear "2006" @default.
• W1974634319 type Work @default.
• W1974634319 sameAs 1974634319 @default.
• W1974634319 citedByCount "21" @default.
• W1974634319 countsByYear W19746343192012 @default.
• W1974634319 countsByYear W19746343192013 @default.
• W1974634319 countsByYear W19746343192014 @default.
• W1974634319 countsByYear W19746343192015 @default.
• W1974634319 crossrefType "journal-article" @default.
• W1974634319 hasAuthorship W1974634319A5035334875 @default.
• W1974634319 hasAuthorship W1974634319A5040514412 @default.
• W1974634319 hasAuthorship W1974634319A5057869475 @default.
• W1974634319 hasAuthorship W1974634319A5061478337 @default.
• W1974634319 hasAuthorship W1974634319A5074852194 @default.
• W1974634319 hasAuthorship W1974634319A5079269717 @default.
• W1974634319 hasConcept C104317684 @default.
• W1974634319 hasConcept C105580179 @default.
• W1974634319 hasConcept C142613039 @default.
• W1974634319 hasConcept C149364088 @default.
• W1974634319 hasConcept C153911025 @default.
• W1974634319 hasConcept C178790620 @default.
• W1974634319 hasConcept C185592680 @default.
• W1974634319 hasConcept C2777563447 @default.
• W1974634319 hasConcept C40767141 @default.
• W1974634319 hasConcept C43617362 @default.
• W1974634319 hasConcept C4692481 @default.
• W1974634319 hasConcept C48023723 @default.
• W1974634319 hasConcept C55493867 @default.
• W1974634319 hasConcept C67705224 @default.
• W1974634319 hasConcept C75442077 @default.
• W1974634319 hasConcept C86803240 @default.
• W1974634319 hasConcept C9893847 @default.
• W1974634319 hasConceptScore W1974634319C104317684 @default.
• W1974634319 hasConceptScore W1974634319C105580179 @default.
• W1974634319 hasConceptScore W1974634319C142613039 @default.
• W1974634319 hasConceptScore W1974634319C149364088 @default.
• W1974634319 hasConceptScore W1974634319C153911025 @default.
• W1974634319 hasConceptScore W1974634319C178790620 @default.
• W1974634319 hasConceptScore W1974634319C185592680 @default.
• W1974634319 hasConceptScore W1974634319C2777563447 @default.
• W1974634319 hasConceptScore W1974634319C40767141 @default.
• W1974634319 hasConceptScore W1974634319C43617362 @default.
• W1974634319 hasConceptScore W1974634319C4692481 @default.
• W1974634319 hasConceptScore W1974634319C48023723 @default.
• W1974634319 hasConceptScore W1974634319C55493867 @default.
• W1974634319 hasConceptScore W1974634319C67705224 @default.
• W1974634319 hasConceptScore W1974634319C75442077 @default.
• W1974634319 hasConceptScore W1974634319C86803240 @default.
• W1974634319 hasConceptScore W1974634319C9893847 @default.
• W1974634319 hasIssue "19" @default.
• W1974634319 hasLocation W19746343191 @default.
• W1974634319 hasLocation W19746343192 @default.
• W1974634319 hasOpenAccess W1974634319 @default.
• W1974634319 hasPrimaryLocation W19746343191 @default.
• W1974634319 hasRelatedWork W1512954123 @default.
• W1974634319 hasRelatedWork W2059288109 @default.
• W1974634319 hasRelatedWork W2060751813 @default.
• W1974634319 hasRelatedWork W2101816396 @default.
• W1974634319 hasRelatedWork W2349802016 @default.
• W1974634319 hasRelatedWork W2370056855 @default.
• W1974634319 hasRelatedWork W2377192522 @default.

• next