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- W1974712209 endingPage "537" @default.
- W1974712209 startingPage "529" @default.
- W1974712209 abstract "• Fucosylated and sialylated oligosaccharides, N -acetylglucosamine and fucose residues are present on bank vole spermatozoa. • Octylphenol modulates glycan expression and its distribution in bank vole spermatozoa. • Octylphenol affects calcium level in bank vole spermatozoa. • Octylphenol induces ultrastructural changes in acrosome region. Our previous studies revealed that in bank vole testicular cells octylphenol (OP) action is related either to its binding to estrogen receptors or increasing cAMP level that modulates spermatozoa motility and acrosome reaction (AR), (Kotula-Balak et al., 2011, 2014). To better understand the mechanisms underlying these changes, in the present study we aimed at evaluating the glycosylation pattern, calcium (Ca 2+ ) level and ultrastructure of OP-treated vole spermatozoa. Glycans were recognized by lectins and localized for the first time on the surface of acrosomal cap and tail of vole spermatozoa. Expression and localization of glycans were determined histochemically and analyzed quantitatively. Following OP the expression of glycans markedly changed with concomitant increase of intracellular Ca 2+ concentration. Altered Ca 2+ signaling pathway and ultrastructural changes in sperm acrosome region were revealed by immunoenzymatic assay and electron microscope analysis together with hypo-osmotic swelling test, respectively. In detail, AR advancement reflected by the presence of small vesicles in the close vicinity to the sperm head was observed, while tail membrane integrity remained intact. Our study provides a new insight on OP direct effects on precocious AR of vole spermatozoa through modulation of the glycan expression and its distribution concomitantly with changes in Ca 2+ signaling pathway and acrosome ultrastructure in vitro ." @default.
- W1974712209 created "2016-06-24" @default.
- W1974712209 creator A5008684210 @default.
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- W1974712209 creator A5045427481 @default.
- W1974712209 creator A5062175602 @default.
- W1974712209 creator A5088103479 @default.
- W1974712209 date "2015-04-01" @default.
- W1974712209 modified "2023-10-14" @default.
- W1974712209 title "Octylphenol induces changes in glycosylation pattern, calcium level and ultrastructure of bank vole spermatozoa in vitro" @default.
- W1974712209 cites W1034470810 @default.
- W1974712209 cites W1542467604 @default.
- W1974712209 cites W1593269210 @default.
- W1974712209 cites W1604980863 @default.
- W1974712209 cites W1775749144 @default.
- W1974712209 cites W180717896 @default.
- W1974712209 cites W1867499521 @default.
- W1974712209 cites W1870327319 @default.
- W1974712209 cites W1896539835 @default.
- W1974712209 cites W1969183351 @default.
- W1974712209 cites W1970496888 @default.
- W1974712209 cites W1972502846 @default.
- W1974712209 cites W1973279184 @default.
- W1974712209 cites W1979339696 @default.
- W1974712209 cites W1982275666 @default.
- W1974712209 cites W1984062230 @default.
- W1974712209 cites W1988626741 @default.
- W1974712209 cites W1990762193 @default.
- W1974712209 cites W1995080280 @default.
- W1974712209 cites W1995091765 @default.
- W1974712209 cites W1995456763 @default.
- W1974712209 cites W1996675568 @default.
- W1974712209 cites W1999955267 @default.
- W1974712209 cites W2002194987 @default.
- W1974712209 cites W2004623873 @default.
- W1974712209 cites W2005940217 @default.
- W1974712209 cites W2006517048 @default.
- W1974712209 cites W2012567464 @default.
- W1974712209 cites W2014754032 @default.
- W1974712209 cites W2017138655 @default.
- W1974712209 cites W2018142242 @default.
- W1974712209 cites W2020212086 @default.
- W1974712209 cites W2020831992 @default.
- W1974712209 cites W2036529911 @default.
- W1974712209 cites W2038972333 @default.
- W1974712209 cites W2039613037 @default.
- W1974712209 cites W2040910215 @default.
- W1974712209 cites W2043096939 @default.
- W1974712209 cites W2043679919 @default.
- W1974712209 cites W2045165226 @default.
- W1974712209 cites W2049721161 @default.
- W1974712209 cites W2057641424 @default.
- W1974712209 cites W2060241333 @default.
- W1974712209 cites W2061982022 @default.
- W1974712209 cites W2064069319 @default.
- W1974712209 cites W2064514917 @default.
- W1974712209 cites W2065480960 @default.
- W1974712209 cites W2067022646 @default.
- W1974712209 cites W2067589028 @default.
- W1974712209 cites W2069134904 @default.
- W1974712209 cites W2070913019 @default.
- W1974712209 cites W2073264207 @default.
- W1974712209 cites W2080266891 @default.
- W1974712209 cites W2081990324 @default.
- W1974712209 cites W2086024753 @default.
- W1974712209 cites W2087953855 @default.
- W1974712209 cites W2093278618 @default.
- W1974712209 cites W2093402385 @default.
- W1974712209 cites W2093453336 @default.
- W1974712209 cites W2094581247 @default.
- W1974712209 cites W2095582725 @default.
- W1974712209 cites W2097734497 @default.
- W1974712209 cites W2098917046 @default.
- W1974712209 cites W2100739494 @default.
- W1974712209 cites W2103627092 @default.
- W1974712209 cites W2107703764 @default.
- W1974712209 cites W2108602150 @default.
- W1974712209 cites W2109557790 @default.
- W1974712209 cites W2118022405 @default.
- W1974712209 cites W2118680793 @default.
- W1974712209 cites W2121901518 @default.
- W1974712209 cites W2126820512 @default.
- W1974712209 cites W2126864948 @default.
- W1974712209 cites W2127098452 @default.
- W1974712209 cites W2128045013 @default.
- W1974712209 cites W2130297851 @default.
- W1974712209 cites W2131811477 @default.
- W1974712209 cites W2137608425 @default.
- W1974712209 cites W2142645963 @default.
- W1974712209 cites W2147518603 @default.
- W1974712209 cites W2148570546 @default.
- W1974712209 cites W2153416567 @default.
- W1974712209 cites W2154700616 @default.
- W1974712209 cites W2159387890 @default.
- W1974712209 cites W2162033352 @default.
- W1974712209 cites W2163102919 @default.
- W1974712209 cites W2163582404 @default.
- W1974712209 cites W2165178028 @default.