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- W1974745573 abstract "Human urinary protease inhibitor was isolated from freshly collected male urine by a single chromatographic step utilizing bovine α-chymotrypsin (EC 3.4.21.1) covalently bound to cross-linked agarose. The adsorbent was prepared by a new series of chemical reactions using trichloro-s-triazine as coupler. Study of chromatographic parameters, including pH, temperature, and ionic strength, led to a simplified elution system for removing contaminants. The antitryptic-antichymotryptic activity of the inhibitor appeared to be unaltered by chromatography." @default.
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- W1974745573 date "1979-01-01" @default.
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- W1974745573 title "Isolation of human urinary protease inhibitor by single-step affinity chromatography" @default.
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- W1974745573 doi "https://doi.org/10.1016/0003-2697(79)90675-4" @default.
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