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- W1974769076 abstract "A complete synthetic protocol as well as 1H- and 13C-NMR data on the monomer building blocks used for the solid-phase synthesis of specifically 13C-labelled (99 atom % 13C) stem (27A and 43G), bulge (24C) and loop (31U) regions of 29mer HIV-1 TAR RNA hairpin starting from the 13C6-D-glucose are presented. The complex NMR spectra of 13C-labelled monomer building blocks, due to the interaction of various 13C and 1H spins, have been assigned. It has been demonstrated by heteronuclear 2D NMR that the non-uniform labelling of the HIV-1 TAR 29mer RNA achieved herein by chemical synthesis provides an optimal opportunity to perform full T1 and T2 relaxation measurements (the “NMR Relaxation Window”) of each type of sugar-carbons for all four strategically placed 13C-labelled residues in a unique and unprecedented manner because of minimal overlap of 13C resonances compared to uniformly labelled oligo-RNA." @default.
- W1974769076 created "2016-06-24" @default.
- W1974769076 creator A5000814916 @default.
- W1974769076 creator A5023551308 @default.
- W1974769076 creator A5027405458 @default.
- W1974769076 creator A5045050279 @default.
- W1974769076 creator A5066355029 @default.
- W1974769076 creator A5068711541 @default.
- W1974769076 date "1999-05-01" @default.
- W1974769076 modified "2023-09-26" @default.
- W1974769076 title "The first example of sequence-specific non-uniformly 13C5 labelled RNA: Synthesis of the 29mer HIV-1 TAR RNA with 13C Relaxation Window" @default.
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