Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974778522> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1974778522 abstract "A long-term goal of work in our laboratories is to understand the structural basis of myofilament function, i.e. cross-bridge dynamics (CD) and myofilament length dependent activation (LDA) in striated muscle in heath and disease. Moreover, in cardiac muscle LDA underlies the Frank Starling Law of the Heart. Troponin phosphorylation, and in particular troponin-I (cTnI), has been suggested to be a pivotal modulator of myofilament function. Here we examined the impact of phosphorylation of distinct cTnI domains on CD and LDA in isolated human myocardium. Site specific phosphorylation was accomplished by charge mutation of hcTnI phosphomimics on the PKA (S23/24D), PKC (S42/44E; T143E), AMPK (S150D), and novel (S5/6D) sites, followed by recombinant protein exchange into skinned non-failing human LV cardiac muscle strips (∼2mm long, and ∼150um diameter. Force and ATPase activity was measured as function of [Ca2+] at short and long sarcomere length (SL=2.0&2.3um). We found, LDA: hcTnI-S150D attenuated, hcTnI-S42/43E increase, and no effect for the other sites. CD as indexed by tension cost was: decreased for cTnI-S42/44E and hcTnI-S150D, and no effect for the other sites. We conclude that cTnI phosphorylation at distinct sites differentially affect cross-bridge cycling and length dependent activation in human myocardium. Structural analysis employing x-ray diffraction is underway to determine the structural basis for these phenomena. Supported by NIH HL075494, HL62426, GM103622." @default.
- W1974778522 created "2016-06-24" @default.
- W1974778522 creator A5025135221 @default.
- W1974778522 creator A5028046659 @default.
- W1974778522 creator A5034292441 @default.
- W1974778522 creator A5056694490 @default.
- W1974778522 creator A5057363714 @default.
- W1974778522 date "2014-01-01" @default.
- W1974778522 modified "2023-09-29" @default.
- W1974778522 title "Impact of Troponin-I Phosphorylation on Human Cardiac Myofilament Function" @default.
- W1974778522 doi "https://doi.org/10.1016/j.bpj.2013.11.4229" @default.
- W1974778522 hasPublicationYear "2014" @default.
- W1974778522 type Work @default.
- W1974778522 sameAs 1974778522 @default.
- W1974778522 citedByCount "0" @default.
- W1974778522 crossrefType "journal-article" @default.
- W1974778522 hasAuthorship W1974778522A5025135221 @default.
- W1974778522 hasAuthorship W1974778522A5028046659 @default.
- W1974778522 hasAuthorship W1974778522A5034292441 @default.
- W1974778522 hasAuthorship W1974778522A5056694490 @default.
- W1974778522 hasAuthorship W1974778522A5057363714 @default.
- W1974778522 hasBestOaLocation W19747785221 @default.
- W1974778522 hasConcept C115725540 @default.
- W1974778522 hasConcept C11960822 @default.
- W1974778522 hasConcept C12554922 @default.
- W1974778522 hasConcept C126322002 @default.
- W1974778522 hasConcept C133927893 @default.
- W1974778522 hasConcept C174054327 @default.
- W1974778522 hasConcept C185592680 @default.
- W1974778522 hasConcept C207200792 @default.
- W1974778522 hasConcept C2778996579 @default.
- W1974778522 hasConcept C36036425 @default.
- W1974778522 hasConcept C500558357 @default.
- W1974778522 hasConcept C55493867 @default.
- W1974778522 hasConcept C68731436 @default.
- W1974778522 hasConcept C6997183 @default.
- W1974778522 hasConcept C71924100 @default.
- W1974778522 hasConcept C7301908 @default.
- W1974778522 hasConcept C86803240 @default.
- W1974778522 hasConcept C95444343 @default.
- W1974778522 hasConceptScore W1974778522C115725540 @default.
- W1974778522 hasConceptScore W1974778522C11960822 @default.
- W1974778522 hasConceptScore W1974778522C12554922 @default.
- W1974778522 hasConceptScore W1974778522C126322002 @default.
- W1974778522 hasConceptScore W1974778522C133927893 @default.
- W1974778522 hasConceptScore W1974778522C174054327 @default.
- W1974778522 hasConceptScore W1974778522C185592680 @default.
- W1974778522 hasConceptScore W1974778522C207200792 @default.
- W1974778522 hasConceptScore W1974778522C2778996579 @default.
- W1974778522 hasConceptScore W1974778522C36036425 @default.
- W1974778522 hasConceptScore W1974778522C500558357 @default.
- W1974778522 hasConceptScore W1974778522C55493867 @default.
- W1974778522 hasConceptScore W1974778522C68731436 @default.
- W1974778522 hasConceptScore W1974778522C6997183 @default.
- W1974778522 hasConceptScore W1974778522C71924100 @default.
- W1974778522 hasConceptScore W1974778522C7301908 @default.
- W1974778522 hasConceptScore W1974778522C86803240 @default.
- W1974778522 hasConceptScore W1974778522C95444343 @default.
- W1974778522 hasLocation W19747785221 @default.
- W1974778522 hasOpenAccess W1974778522 @default.
- W1974778522 hasPrimaryLocation W19747785221 @default.
- W1974778522 hasRelatedWork W1976489305 @default.
- W1974778522 hasRelatedWork W1980963845 @default.
- W1974778522 hasRelatedWork W1986986844 @default.
- W1974778522 hasRelatedWork W1996387758 @default.
- W1974778522 hasRelatedWork W1998479437 @default.
- W1974778522 hasRelatedWork W2009953198 @default.
- W1974778522 hasRelatedWork W2011583811 @default.
- W1974778522 hasRelatedWork W2025759932 @default.
- W1974778522 hasRelatedWork W2030703742 @default.
- W1974778522 hasRelatedWork W2031417439 @default.
- W1974778522 hasRelatedWork W2033654361 @default.
- W1974778522 hasRelatedWork W2033776976 @default.
- W1974778522 hasRelatedWork W2062508731 @default.
- W1974778522 hasRelatedWork W2076996105 @default.
- W1974778522 hasRelatedWork W2089408545 @default.
- W1974778522 hasRelatedWork W2132932675 @default.
- W1974778522 hasRelatedWork W2140454827 @default.
- W1974778522 hasRelatedWork W2188578812 @default.
- W1974778522 hasRelatedWork W2513378292 @default.
- W1974778522 hasRelatedWork W2684293040 @default.
- W1974778522 isParatext "false" @default.
- W1974778522 isRetracted "false" @default.
- W1974778522 magId "1974778522" @default.
- W1974778522 workType "article" @default.