Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974794664> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1974794664 endingPage "1521" @default.
- W1974794664 startingPage "1515" @default.
- W1974794664 abstract "Amadoriases are a novel class of FAD enzymes which catalyze the oxidative deglycation of glycated amino acids to yield corresponding amino acids, glucosone, and H(2)O(2). We previously reported the purification and characterization of two amadoriase isoenzymes from Aspergillus fumigatus and the molecular cloning of amadoriase II. To identify the primary structure of amadoriase I, we prepared a cDNA library from Aspergillus fumigatus and isolated a clone using a probe amplified by polymerase chain reaction with primers designed according to the partial amino acid sequences from peptide mapping. The primary structure of the enzyme deduced from the nucleotide sequence comprises 445 amino acid residues. The enzyme contains 1 mol of FAD as a cofactor, which is covalently linked to Cys342, as determined by mutagenesis analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and electrospray ionization-collisional-activated dissociation tandem mass spectrometry. Sequence alignment studies show that amadoriase I has 22% homology with monomeric sarcosine oxidase in which FAD is also linked to a homologous Cys residue. Amadoriases are of potential importance as tools for uncoupling hyperglycemia and glycation reactions that are thought to play a role in diabetic complications." @default.
- W1974794664 created "2016-06-24" @default.
- W1974794664 creator A5048439423 @default.
- W1974794664 creator A5049385707 @default.
- W1974794664 creator A5058558025 @default.
- W1974794664 creator A5066304823 @default.
- W1974794664 date "2000-01-19" @default.
- W1974794664 modified "2023-09-26" @default.
- W1974794664 title "Cloning of Amadoriase I Isoenzyme from <i>Aspergillus</i> <i>sp</i>.: Evidence of FAD Covalently Linked to Cys342<sup>,</sup>" @default.
- W1974794664 cites W1518714662 @default.
- W1974794664 cites W1609735481 @default.
- W1974794664 cites W1968332280 @default.
- W1974794664 cites W2008000048 @default.
- W1974794664 cites W2031503758 @default.
- W1974794664 cites W2069942321 @default.
- W1974794664 cites W2073931886 @default.
- W1974794664 cites W2099191400 @default.
- W1974794664 doi "https://doi.org/10.1021/bi992031g" @default.
- W1974794664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10684633" @default.
- W1974794664 hasPublicationYear "2000" @default.
- W1974794664 type Work @default.
- W1974794664 sameAs 1974794664 @default.
- W1974794664 citedByCount "36" @default.
- W1974794664 countsByYear W19747946642012 @default.
- W1974794664 countsByYear W19747946642013 @default.
- W1974794664 countsByYear W19747946642015 @default.
- W1974794664 countsByYear W19747946642016 @default.
- W1974794664 countsByYear W19747946642017 @default.
- W1974794664 countsByYear W19747946642021 @default.
- W1974794664 crossrefType "journal-article" @default.
- W1974794664 hasAuthorship W1974794664A5048439423 @default.
- W1974794664 hasAuthorship W1974794664A5049385707 @default.
- W1974794664 hasAuthorship W1974794664A5058558025 @default.
- W1974794664 hasAuthorship W1974794664A5066304823 @default.
- W1974794664 hasConcept C104317684 @default.
- W1974794664 hasConcept C162356407 @default.
- W1974794664 hasConcept C167625842 @default.
- W1974794664 hasConcept C181199279 @default.
- W1974794664 hasConcept C185592680 @default.
- W1974794664 hasConcept C197957613 @default.
- W1974794664 hasConcept C2775859393 @default.
- W1974794664 hasConcept C2779787849 @default.
- W1974794664 hasConcept C40684141 @default.
- W1974794664 hasConcept C43617362 @default.
- W1974794664 hasConcept C515207424 @default.
- W1974794664 hasConcept C55493867 @default.
- W1974794664 hasConcept C86803240 @default.
- W1974794664 hasConcept C89423630 @default.
- W1974794664 hasConceptScore W1974794664C104317684 @default.
- W1974794664 hasConceptScore W1974794664C162356407 @default.
- W1974794664 hasConceptScore W1974794664C167625842 @default.
- W1974794664 hasConceptScore W1974794664C181199279 @default.
- W1974794664 hasConceptScore W1974794664C185592680 @default.
- W1974794664 hasConceptScore W1974794664C197957613 @default.
- W1974794664 hasConceptScore W1974794664C2775859393 @default.
- W1974794664 hasConceptScore W1974794664C2779787849 @default.
- W1974794664 hasConceptScore W1974794664C40684141 @default.
- W1974794664 hasConceptScore W1974794664C43617362 @default.
- W1974794664 hasConceptScore W1974794664C515207424 @default.
- W1974794664 hasConceptScore W1974794664C55493867 @default.
- W1974794664 hasConceptScore W1974794664C86803240 @default.
- W1974794664 hasConceptScore W1974794664C89423630 @default.
- W1974794664 hasIssue "6" @default.
- W1974794664 hasLocation W19747946641 @default.
- W1974794664 hasLocation W19747946642 @default.
- W1974794664 hasOpenAccess W1974794664 @default.
- W1974794664 hasPrimaryLocation W19747946641 @default.
- W1974794664 hasRelatedWork W1164537646 @default.
- W1974794664 hasRelatedWork W1644178486 @default.
- W1974794664 hasRelatedWork W1987636796 @default.
- W1974794664 hasRelatedWork W2003889801 @default.
- W1974794664 hasRelatedWork W2080203695 @default.
- W1974794664 hasRelatedWork W2091146030 @default.
- W1974794664 hasRelatedWork W2098689981 @default.
- W1974794664 hasRelatedWork W2162473589 @default.
- W1974794664 hasRelatedWork W2524883418 @default.
- W1974794664 hasRelatedWork W2613132418 @default.
- W1974794664 hasVolume "39" @default.
- W1974794664 isParatext "false" @default.
- W1974794664 isRetracted "false" @default.
- W1974794664 magId "1974794664" @default.
- W1974794664 workType "article" @default.