Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974820747> ?p ?o ?g. }
- W1974820747 endingPage "458" @default.
- W1974820747 startingPage "448" @default.
- W1974820747 abstract "Epidermal keratinocytes are critical targets for UV-induced genotoxicity as their transformation by sunlight overexposure can lead to skin cancer such as basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Therefore, assessment of photoprotection should involve early markers associated with DNA photodamage. Here, the same normal human keratinocytes either in monoculture (KC) or in full thickness reconstructed skin (RS) were compared with respect to their response to simulated solar UV (SSUV) exposure. Irradiation conditions (spectral power distribution and doses) were designed to mimic environmental zenithal UV from sunlight. At doses where survival was higher than 80%, comet assay showed more single strand breaks (SSB) and cyclobutane pyrimidine dimers (CPD) in keratinocytes in RS than in KC one hour post-exposure. The transcription factor p53 was activated in both models. While in KC p53 accumulation displayed a linear dose-dependency up to 24 h post-exposure, in RS it followed a bell-shaped profile and reverted to its basal rate. QRT-PCR demonstrated that among genes controlled by p53, P21 and MDM2 were clearly induced by SSUV in KC, whereas GADD45 expression was strongly and almost exclusively up-regulated in RS. Nrf2-dependent antioxidant genes (Ferritin light chain, NQO1) were only induced in RS, yet at low doses for NQO1. In vitro models such as KC or RS allowing the development of quantitative methodologies should be used as surrogates for in vivo tests assessing photogenotoxicity." @default.
- W1974820747 created "2016-06-24" @default.
- W1974820747 creator A5032021807 @default.
- W1974820747 creator A5036123727 @default.
- W1974820747 creator A5039327152 @default.
- W1974820747 creator A5081458575 @default.
- W1974820747 creator A5083672933 @default.
- W1974820747 creator A5084965183 @default.
- W1974820747 date "2010-04-01" @default.
- W1974820747 modified "2023-10-16" @default.
- W1974820747 title "In vitro tools for photobiological testing: molecular responses to simulated solar UV of keratinocytes growing as monolayers or as part of reconstructed skin" @default.
- W1974820747 cites W1488294447 @default.
- W1974820747 cites W1539398224 @default.
- W1974820747 cites W1596182601 @default.
- W1974820747 cites W1826137220 @default.
- W1974820747 cites W1927414006 @default.
- W1974820747 cites W1964190328 @default.
- W1974820747 cites W1967739837 @default.
- W1974820747 cites W1973466074 @default.
- W1974820747 cites W1973872210 @default.
- W1974820747 cites W1978058355 @default.
- W1974820747 cites W1981121984 @default.
- W1974820747 cites W1981823569 @default.
- W1974820747 cites W1983644941 @default.
- W1974820747 cites W1987784120 @default.
- W1974820747 cites W1993411969 @default.
- W1974820747 cites W1994322894 @default.
- W1974820747 cites W1995653643 @default.
- W1974820747 cites W2003006686 @default.
- W1974820747 cites W2003302711 @default.
- W1974820747 cites W2007835060 @default.
- W1974820747 cites W2014100758 @default.
- W1974820747 cites W2024126937 @default.
- W1974820747 cites W2025161791 @default.
- W1974820747 cites W2040732896 @default.
- W1974820747 cites W2050631951 @default.
- W1974820747 cites W2054433010 @default.
- W1974820747 cites W2054764151 @default.
- W1974820747 cites W2057158408 @default.
- W1974820747 cites W2061353993 @default.
- W1974820747 cites W2064476022 @default.
- W1974820747 cites W2064512387 @default.
- W1974820747 cites W2065908490 @default.
- W1974820747 cites W2066589243 @default.
- W1974820747 cites W2068501538 @default.
- W1974820747 cites W2070030880 @default.
- W1974820747 cites W2070855956 @default.
- W1974820747 cites W2078023746 @default.
- W1974820747 cites W2081660598 @default.
- W1974820747 cites W2083707704 @default.
- W1974820747 cites W2087406053 @default.
- W1974820747 cites W2095446192 @default.
- W1974820747 cites W2100130506 @default.
- W1974820747 cites W2103797279 @default.
- W1974820747 cites W2108244474 @default.
- W1974820747 cites W2113716833 @default.
- W1974820747 cites W2145798951 @default.
- W1974820747 cites W2186368357 @default.
- W1974820747 cites W2219047851 @default.
- W1974820747 cites W2300559698 @default.
- W1974820747 doi "https://doi.org/10.1039/b9pp00145j" @default.
- W1974820747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20354637" @default.
- W1974820747 hasPublicationYear "2010" @default.
- W1974820747 type Work @default.
- W1974820747 sameAs 1974820747 @default.
- W1974820747 citedByCount "15" @default.
- W1974820747 countsByYear W19748207472012 @default.
- W1974820747 countsByYear W19748207472013 @default.
- W1974820747 countsByYear W19748207472014 @default.
- W1974820747 countsByYear W19748207472016 @default.
- W1974820747 countsByYear W19748207472017 @default.
- W1974820747 countsByYear W19748207472018 @default.
- W1974820747 countsByYear W19748207472019 @default.
- W1974820747 countsByYear W19748207472020 @default.
- W1974820747 countsByYear W19748207472023 @default.
- W1974820747 crossrefType "journal-article" @default.
- W1974820747 hasAuthorship W1974820747A5032021807 @default.
- W1974820747 hasAuthorship W1974820747A5036123727 @default.
- W1974820747 hasAuthorship W1974820747A5039327152 @default.
- W1974820747 hasAuthorship W1974820747A5081458575 @default.
- W1974820747 hasAuthorship W1974820747A5083672933 @default.
- W1974820747 hasAuthorship W1974820747A5084965183 @default.
- W1974820747 hasBestOaLocation W19748207471 @default.
- W1974820747 hasConcept C12554922 @default.
- W1974820747 hasConcept C143425029 @default.
- W1974820747 hasConcept C153911025 @default.
- W1974820747 hasConcept C167975912 @default.
- W1974820747 hasConcept C178790620 @default.
- W1974820747 hasConcept C183688256 @default.
- W1974820747 hasConcept C185592680 @default.
- W1974820747 hasConcept C202751555 @default.
- W1974820747 hasConcept C207001950 @default.
- W1974820747 hasConcept C2777074287 @default.
- W1974820747 hasConcept C2777459323 @default.
- W1974820747 hasConcept C2778959862 @default.
- W1974820747 hasConcept C2780358455 @default.
- W1974820747 hasConcept C2780542282 @default.
- W1974820747 hasConcept C29730261 @default.