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- W1974841128 abstract "Fluorometric methods are described for the determination of the enzymes hexokinase and β-glucosidase, and the carbohydrates glucose, fructose, maltose, cellobiose, lactose, glycogen, and salicin Glucose and fructose are determined fluorometrically using hexokinase and the resazurin-resorufin indicator reaction. Maltose, cellobiose, lactose, glycogen, and salicin are enzymically hydrolyzed to glucose, which is then determined fluorometrically using glucose oxidase, p-hydroxyphenylacetic acid, and peroxidase. All the carbohydrates were assayed in the range 0.01–50 μg/ml and the enzymes hexokinase and β-glucosidase in the range 10−3 to 10−1 unit/ml with an accuracy and precision of about 1.5%." @default.
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- W1974841128 date "1969-01-01" @default.
- W1974841128 modified "2023-09-23" @default.
- W1974841128 title "Fluorometric determination of carbohydrates" @default.
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- W1974841128 doi "https://doi.org/10.1016/0003-2697(69)90245-0" @default.
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