FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974846959> ?p ?o ?g. }

• W1974846959 endingPage "486" @default.
• W1974846959 startingPage "477" @default.
• W1974846959 abstract "1.|Preparations of pig kidney alkaline phosphatase (EC 3.1.3.1) were shown to be homogeneous on polyacrylamide-gel electrophoresis. 2.|The apparent molecular weight of the enzyme determined by gel filtration was approx. 185 000. After treatment with sodium dodecylsulphate, a single band with an apparent molecular weight of 80 000–90 000 was identified in polyacrylamide gels containing sodium dodecylsulphate. Thus pig kidney alkaline phosphatase seems to be composed of two subunits. 3.|Chemical cross-linking with glutaraldehyde or dimethyl suberimidate prior to electrophoresis in polyacrylamide-sodium dodecylsulphate gels gave rise to two bands identified as free subunits and cross-linked dimers. This evidence supports the suggestion that pig kidney alkaline phosphatase (like the enzymes from human placenta, calf intestine and Escherichia coli) consists of two very similar, probably identical, subunits. 4.|Titrations of the enzyme with 32Pi at pH 5.0 (where phosphorylated enzyme is relatively stable) indicated that preparations were 45% pure, assuming that the subunits were identical and that two active sites were operational during the titration. If only one active site per molecule becomes phosphorylated then the purity would be 90%. 5.|There was no evidence of negative cooperativity in the binding studies with Pi but extreme negative cooperativity leading to expression of only one site per dimer could not be excluded. As the electrophoresis results seemed to indicate that the purity of the preparations was greater than 45%, there is some support for the belief that only one site was active." @default.
• W1974846959 created "2016-06-24" @default.
• W1974846959 creator A5037308009 @default.
• W1974846959 creator A5070244741 @default.
• W1974846959 date "1974-12-01" @default.
• W1974846959 modified "2023-09-27" @default.
• W1974846959 title "Subunit structure and catalytic activity of pig kidney alkaline phosphatase" @default.
• W1974846959 cites W109670066 @default.
• W1974846959 cites W127973334 @default.
• W1974846959 cites W128882647 @default.
• W1974846959 cites W1484792585 @default.
• W1974846959 cites W1497743504 @default.
• W1974846959 cites W1546726147 @default.
• W1974846959 cites W1599175061 @default.
• W1974846959 cites W1625660792 @default.
• W1974846959 cites W1775749144 @default.
• W1974846959 cites W1867117224 @default.
• W1974846959 cites W1969404194 @default.
• W1974846959 cites W1977723901 @default.
• W1974846959 cites W1978355684 @default.
• W1974846959 cites W1989718572 @default.
• W1974846959 cites W1992811920 @default.
• W1974846959 cites W1998866624 @default.
• W1974846959 cites W2019813586 @default.
• W1974846959 cites W2051967250 @default.
• W1974846959 cites W2056187546 @default.
• W1974846959 cites W2060906023 @default.
• W1974846959 cites W2073879684 @default.
• W1974846959 cites W2111301002 @default.
• W1974846959 cites W2137302959 @default.
• W1974846959 cites W2148885411 @default.
• W1974846959 cites W2397822050 @default.
• W1974846959 cites W2399205053 @default.
• W1974846959 cites W60401492 @default.
• W1974846959 doi "https://doi.org/10.1016/0005-2744(74)90109-0" @default.
• W1974846959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4216366" @default.
• W1974846959 hasPublicationYear "1974" @default.
• W1974846959 type Work @default.
• W1974846959 sameAs 1974846959 @default.
• W1974846959 citedByCount "17" @default.
• W1974846959 crossrefType "journal-article" @default.
• W1974846959 hasAuthorship W1974846959A5037308009 @default.
• W1974846959 hasAuthorship W1974846959A5070244741 @default.
• W1974846959 hasConcept C101660505 @default.
• W1974846959 hasConcept C104292427 @default.
• W1974846959 hasConcept C104317684 @default.
• W1974846959 hasConcept C116084860 @default.
• W1974846959 hasConcept C160160445 @default.
• W1974846959 hasConcept C166014724 @default.
• W1974846959 hasConcept C178666793 @default.
• W1974846959 hasConcept C178790620 @default.
• W1974846959 hasConcept C179104552 @default.
• W1974846959 hasConcept C181199279 @default.
• W1974846959 hasConcept C184866935 @default.
• W1974846959 hasConcept C185592680 @default.
• W1974846959 hasConcept C193363816 @default.
• W1974846959 hasConcept C2779546866 @default.
• W1974846959 hasConcept C2780025047 @default.
• W1974846959 hasConcept C43617362 @default.
• W1974846959 hasConcept C537181965 @default.
• W1974846959 hasConcept C55493867 @default.
• W1974846959 hasConcept C7927669 @default.
• W1974846959 hasConceptScore W1974846959C101660505 @default.
• W1974846959 hasConceptScore W1974846959C104292427 @default.
• W1974846959 hasConceptScore W1974846959C104317684 @default.
• W1974846959 hasConceptScore W1974846959C116084860 @default.
• W1974846959 hasConceptScore W1974846959C160160445 @default.
• W1974846959 hasConceptScore W1974846959C166014724 @default.
• W1974846959 hasConceptScore W1974846959C178666793 @default.
• W1974846959 hasConceptScore W1974846959C178790620 @default.
• W1974846959 hasConceptScore W1974846959C179104552 @default.
• W1974846959 hasConceptScore W1974846959C181199279 @default.
• W1974846959 hasConceptScore W1974846959C184866935 @default.
• W1974846959 hasConceptScore W1974846959C185592680 @default.
• W1974846959 hasConceptScore W1974846959C193363816 @default.
• W1974846959 hasConceptScore W1974846959C2779546866 @default.
• W1974846959 hasConceptScore W1974846959C2780025047 @default.
• W1974846959 hasConceptScore W1974846959C43617362 @default.
• W1974846959 hasConceptScore W1974846959C537181965 @default.
• W1974846959 hasConceptScore W1974846959C55493867 @default.
• W1974846959 hasConceptScore W1974846959C7927669 @default.
• W1974846959 hasIssue "2" @default.
• W1974846959 hasLocation W19748469591 @default.
• W1974846959 hasLocation W19748469592 @default.
• W1974846959 hasOpenAccess W1974846959 @default.
• W1974846959 hasPrimaryLocation W19748469591 @default.
• W1974846959 hasRelatedWork W1549790155 @default.
• W1974846959 hasRelatedWork W1969451414 @default.
• W1974846959 hasRelatedWork W1986569837 @default.
• W1974846959 hasRelatedWork W1996523411 @default.
• W1974846959 hasRelatedWork W2056715324 @default.
• W1974846959 hasRelatedWork W2077552296 @default.
• W1974846959 hasRelatedWork W2089318461 @default.
• W1974846959 hasRelatedWork W2113645490 @default.
• W1974846959 hasRelatedWork W2407636999 @default.
• W1974846959 hasRelatedWork W24551121 @default.
• W1974846959 hasVolume "370" @default.
• W1974846959 isParatext "false" @default.

• next