FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974857996> ?p ?o ?g. }

• W1974857996 endingPage "B61" @default.
• W1974857996 startingPage "B49" @default.
• W1974857996 abstract "This study assessed the performance characteristics of a new microparticle enzyme immunoassay (MEIA) for the determination of sirolimus in whole blood.In clinical investigatory studies, dose adjustments of the immunosuppressive drug sirolimus have been carried out using either high-performance liquid chromatography (HPLC) or, more recently, this investigational immunoassay kit based on the MEIA technique.Calibration was made over the linear range 0 to 30 ng/mL. Inaccuracy and imprecision were assessed by means of 3 control samples supplied with the kit (5, 11, and 22 ng/mL) and dilution of an above-quantitation-limit sample (154 ng/mL). Specificity was determined by the addition of 2 sirolimus metabolites to sirolimus-free human whole blood or to I of the control samples supplied with the kit. In addition, whole-blood samples from patients receiving either cyclosporine or tacrolimus (N = 24) were analyzed for sirolimus. A comparison of the MEIA and a validated HPLC/MS/MS assay analyzed both pooled samples from patients receiving sirolimus and spiked samples (sirolimus 2-60 ng/mL). In a more extensive comparison of patient samples measured by the MEIA assay, a validated HPLC assay with UV detection (HPLC-UV) was used (HPLC-UV sirolimus 7-64 ng/mL).Inaccuracy (between-run) was < or =16.2% at all 4 concentrations (N = 5). Within-assay imprecision (repeatability) was <6% (N = 5), and between-assay imprecision (reproducibility) for the same samples was < 11% (N = 5). Recovery, assessed by means of 3 in-house control samples prepared in both fresh and previously frozen sirolimus-free human whole blood, ranged from 93.9% to 109.5%. The limit of detection, determined by dilution of the lowest nonzero calibrator (3 ng/mL), was set at 1 ng/mL, at which repeatability was 20.5% (N = 5). Five ng/mL of hydroxysirolimus cross-reacted with the assay by a mean of between 44% and 50% (N = 4); 5 ng/mL of 41-O-demethylsirolimus cross-reacted with the assay by a mean of between 86% and 127% (N = 4). Assay specificity was further challenged by ethylenediamine-tetraacetic acid (EDTA)-whole-blood samples from transplant patients not receiving sirolimus. These samples had tacrolimus and cyclosporine concentrations of 7.8 to 15.9 ng/mL and 38 to 485 microg/L, respectively. The median result was 0 ng/mL (third quartile, 0.7 ng/mL; maximum, 1.4 ng/mL); no value was above the lowest nonzero calibrator. The results of the comparison between the MEIA and the HPLC/MS/MS assay showed mean positive biases of 21% and 8% for the MEIA in measuring sirolimus in pooled patient samples and spiked samples, respectively. The results of the comparison of the MEIA and HPLC-UV median sirolimus concentrations were 18.2 and 20.1. Whole-blood samples anticoagulated with EDTA and containing sirolimus were stable for analysis by MEIA for 3 freeze-thaw cycles when stored at -20 degrees C and for 10 days when stored at 4 degrees C or at ambient temperature. A decline in sirolimus concentration occurred when samples were stored at 37 degrees C.The MEIA showed suitable precision across a clinically relevant concentration range. In terms of patient management, the practical significance of cross-reactivity with sirolimus metabolites remains to be assessed." @default.
• W1974857996 created "2016-06-24" @default.
• W1974857996 creator A5010878214 @default.
• W1974857996 creator A5013583738 @default.
• W1974857996 creator A5024846754 @default.
• W1974857996 creator A5026759719 @default.
• W1974857996 creator A5031108726 @default.
• W1974857996 creator A5068378157 @default.
• W1974857996 creator A5080041620 @default.
• W1974857996 date "2000-01-01" @default.
• W1974857996 modified "2023-09-27" @default.
• W1974857996 title "An immunoassay for the measurement of sirolimus" @default.
• W1974857996 cites W1519282657 @default.
• W1974857996 cites W1872431240 @default.
• W1974857996 cites W1948019784 @default.
• W1974857996 cites W1964662082 @default.
• W1974857996 cites W1975545753 @default.
• W1974857996 cites W1996206538 @default.
• W1974857996 cites W1999043049 @default.
• W1974857996 cites W2005159788 @default.
• W1974857996 cites W2011010559 @default.
• W1974857996 cites W2024839583 @default.
• W1974857996 cites W2027196853 @default.
• W1974857996 cites W2038088215 @default.
• W1974857996 cites W2040432867 @default.
• W1974857996 cites W2053378671 @default.
• W1974857996 cites W2070331777 @default.
• W1974857996 cites W2071307134 @default.
• W1974857996 cites W2088661762 @default.
• W1974857996 cites W2109039071 @default.
• W1974857996 cites W2167221091 @default.
• W1974857996 doi "https://doi.org/10.1016/s0149-2918(00)89022-0" @default.
• W1974857996 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10823373" @default.
• W1974857996 hasPublicationYear "2000" @default.
• W1974857996 type Work @default.
• W1974857996 sameAs 1974857996 @default.
• W1974857996 citedByCount "53" @default.
• W1974857996 countsByYear W19748579962012 @default.
• W1974857996 countsByYear W19748579962013 @default.
• W1974857996 countsByYear W19748579962016 @default.
• W1974857996 countsByYear W19748579962020 @default.
• W1974857996 countsByYear W19748579962022 @default.
• W1974857996 crossrefType "journal-article" @default.
• W1974857996 hasAuthorship W1974857996A5010878214 @default.
• W1974857996 hasAuthorship W1974857996A5013583738 @default.
• W1974857996 hasAuthorship W1974857996A5024846754 @default.
• W1974857996 hasAuthorship W1974857996A5026759719 @default.
• W1974857996 hasAuthorship W1974857996A5031108726 @default.
• W1974857996 hasAuthorship W1974857996A5068378157 @default.
• W1974857996 hasAuthorship W1974857996A5080041620 @default.
• W1974857996 hasConcept C112705442 @default.
• W1974857996 hasConcept C126322002 @default.
• W1974857996 hasConcept C154020017 @default.
• W1974857996 hasConcept C159654299 @default.
• W1974857996 hasConcept C179998833 @default.
• W1974857996 hasConcept C185592680 @default.
• W1974857996 hasConcept C203014093 @default.
• W1974857996 hasConcept C2777921159 @default.
• W1974857996 hasConcept C2780272996 @default.
• W1974857996 hasConcept C2780420688 @default.
• W1974857996 hasConcept C43617362 @default.
• W1974857996 hasConcept C71924100 @default.
• W1974857996 hasConcept C98274493 @default.
• W1974857996 hasConcept C9893847 @default.
• W1974857996 hasConceptScore W1974857996C112705442 @default.
• W1974857996 hasConceptScore W1974857996C126322002 @default.
• W1974857996 hasConceptScore W1974857996C154020017 @default.
• W1974857996 hasConceptScore W1974857996C159654299 @default.
• W1974857996 hasConceptScore W1974857996C179998833 @default.
• W1974857996 hasConceptScore W1974857996C185592680 @default.
• W1974857996 hasConceptScore W1974857996C203014093 @default.
• W1974857996 hasConceptScore W1974857996C2777921159 @default.
• W1974857996 hasConceptScore W1974857996C2780272996 @default.
• W1974857996 hasConceptScore W1974857996C2780420688 @default.
• W1974857996 hasConceptScore W1974857996C43617362 @default.
• W1974857996 hasConceptScore W1974857996C71924100 @default.
• W1974857996 hasConceptScore W1974857996C98274493 @default.
• W1974857996 hasConceptScore W1974857996C9893847 @default.
• W1974857996 hasLocation W19748579961 @default.
• W1974857996 hasLocation W19748579962 @default.
• W1974857996 hasOpenAccess W1974857996 @default.
• W1974857996 hasPrimaryLocation W19748579961 @default.
• W1974857996 hasRelatedWork W1871281899 @default.
• W1974857996 hasRelatedWork W1970475868 @default.
• W1974857996 hasRelatedWork W1985896768 @default.
• W1974857996 hasRelatedWork W1999043049 @default.
• W1974857996 hasRelatedWork W2039294618 @default.
• W1974857996 hasRelatedWork W2171058574 @default.
• W1974857996 hasRelatedWork W2352674505 @default.
• W1974857996 hasRelatedWork W2360553150 @default.
• W1974857996 hasRelatedWork W2364492369 @default.
• W1974857996 hasRelatedWork W2601524031 @default.
• W1974857996 hasVolume "22" @default.
• W1974857996 isParatext "false" @default.
• W1974857996 isRetracted "false" @default.
• W1974857996 magId "1974857996" @default.
• W1974857996 workType "article" @default.